Phenotypes associated with this allele

• Allele Symbol: Cripto^tm2Mms
• Allele Name: targeted mutation 2, Michael M Shen
• Allele ID: MGI:3612967
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Cripto^tm1Mms/Cripto^tm2Mms	involves: 129S6/SvEvTac * C57BL/6	MGI:3613772
ht2	Cripto^tm2Mms/Cripto^tm2.1Mms	involves: 129S6/SvEvTac * C57BL/6	MGI:3613773

Genotype
MGI:3613772

ht1

• Allelic Composition: Cripto^tm1Mms/Cripto^tm2Mms
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:104418 )

• fewer than expected mutants are found at 4 weeks of age (8% rather than 25%)

embryo

abnormal endoderm development ( J:104418 )

• at E7.5 expression of Hex, a marker of anterior definitive endoderm, is often undetectable; however Hex expression in visceral endoderm is normal

• at E8.25 expression of Shh marking ventral midline structures and gut endoderm is greatly reduced or absent

• these changes in expression indicate defects in axial mesendoderm and definitive endoderm development with a nearly complete loss of definitive endoderm in severely affected embryos

abnormal mesendoderm development ( J:104418 )

• defects in axial mesendoderm development

rostral body truncation ( J:104418 )

• severely affected embryos show anterior truncations

• less affected embryos display anterior restricted phenotypes

absent anterior definitive endoderm ( J:104418 )

• at E7.5 expression of Hex, a marker of anterior definitive endoderm, is often undetectable

absent notochord ( J:104418 )

• in severely affected embryos the anterior somites are fused across the midline indicating the absence of the notochord

absent prechordal plate ( J:104418 )

• at E7.75 an intact prechordal plate fails to form

fused somites ( J:104418 )

• in severely affected embryos the anterior somites are fused across the midline indicating the absence of the notochord

nervous system

holoprosencephaly ( J:104418 )

• at E8.25 - E9.5, about 56% display a broad spectrum of phenotypes with characteristic features of holoprosencephaly

• at E11.5 severely affected embryos have a single prosencephalic vesicle

decreased forebrain size ( J:104418 )

• at E9.0 - E11.5 less severely affected embryos have variable forebrain reductions including truncation of the rostral forebrain

decreased midbrain size ( J:104418 )

• at E9.0 - E11.5 less severely affected embryos have variable midbrain reductions

vision/eye

cyclopia ( J:104418 )

• seen in less severely affected embryos

Genotype
MGI:3613773

ht2

• Allelic Composition: Cripto^tm2Mms/Cripto^tm2.1Mms
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

mortality/aging

premature death ( J:104418 )

• fewer than expected mutants are found at 4 weeks of age (8% rather than 25%)

embryo

abnormal endoderm development ( J:104418 )

• at E7.5 expression of Hex, a marker of anterior definitive endoderm, is often undetectable; however Hex expression in visceral endoderm is normal

• at E8.25 expression of Shh marking ventral midline structures and gut endoderm is greatly reduced or absent

• these changes in expression indicate defects in axial mesendoderm and definitive endoderm development with a nearly complete loss of definitive endoderm in severely affected embryos

abnormal mesendoderm development ( J:104418 )

• defects in axial mesendoderm development

rostral body truncation ( J:104418 )

• severely affected embryos show anterior truncations

• less affected embryos display anterior restricted phenotypes

absent anterior definitive endoderm ( J:104418 )

• at E7.5 expression of Hex, a marker of anterior definitive endoderm, is often undetectable

absent notochord ( J:104418 )

• in severely affected embryos the anterior somites are fused across the midline indicating the absence of the notochord

absent prechordal plate ( J:104418 )

• at E7.75 an intact prechordal plate fails to form

fused somites ( J:104418 )

• in severely affected embryos the anterior somites are fused across the midline indicating the absence of the notochord

nervous system

holoprosencephaly ( J:104418 )

• at E8.25 - E9.5, about 56% display a broad spectrum of phenotypes with characteristic features of holoprosencephaly

• at E11.5 severely affected embryos have a single prosencephalic vesicle

decreased forebrain size ( J:104418 )

• at E9.0 - E11.5 less severely affected embryos have variable forebrain reductions including truncation of the rostral forebrain

decreased midbrain size ( J:104418 )

• at E9.0 - E11.5 less severely affected embryos have variable midbrain reductions

vision/eye

cyclopia ( J:104418 )

• seen in less severely affected embryos