Phenotypes associated with this allele

• Allele Symbol: Grin1^tm1Rsp
• Allele Name: targeted mutation 1, Rolf Sprengel
• Allele ID: MGI:3611337
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grin1^tm1Rsp/Grin1^tm1Rsp Tg(Slc6a3-icre)9075Gsc/0	involves: 129S1/Sv * 129X1/SvJ	MGI:3835942
cn2	Grin1^tm1Rsp/Grin1^tm1Rsp Tg(Camk2a-tTA)1Mmay/? Tg(tetO-cre)LC1Bjd/?	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6	MGI:3708939
cn3	Grin1^tm1Rsp/Grin1^tm1Rsp Tg(Slc6a3-icre)9075Gsc/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:3835859
cn4	Grin1^tm1Rsp/Grin1^tm1Rsp Tg(Gnrh1-cre)1Rsp/0	involves: C57BL/6	MGI:3612448

Genotype
MGI:3835942

cn1

• Allelic Composition: Grin1^tm1Rsp/Grin1^tm1Rsp; Tg(Slc6a3-icre)9075Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal glutamate-mediated receptor currents ( J:139693 )

• cocaine-induced synaptic strengthening in the ventral tegmental area does not occur in mice after cre activity is induced

abnormal NMDA-mediated synaptic currents ( J:139693 )

• NMDA-mediated excitatory postsynaptic currents are completely abolished in the ventral tegmental area of mice after cre activity is induced

behavior/neurological

impaired behavioral response to cocaine ( J:139693 )

• mice that have had cre activity induced and were conditioned to associate a certain cage environment with cocaine do not reinstate their cocaine-seeking behavior when administered the drug after a 16 day extinction phase

Genotype
MGI:3708939

cn2

• Allelic Composition: Grin1^tm1Rsp/Grin1^tm1Rsp; Tg(Camk2a-tTA)1Mmay/?; Tg(tetO-cre)LC1Bjd/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6

nervous system

abnormal long-term potentiation ( J:119834 )

• complete loss of NMDAR-mediated long term potential at dentate gyrus synapses

• however, long term potentiation at Schaffer collateral-CA1 synapses is similar to wild-type mice despite residual Cre activity in CA1 pyramidal cells

behavior/neurological

abnormal spatial working memory ( J:119834 )

• mice exhibit impaired spatial working memory but normal spatial reference memory in a 3 from 6 radial arm maze task

Genotype
MGI:3835859

cn3

• Allelic Composition: Grin1^tm1Rsp/Grin1^tm1Rsp; Tg(Slc6a3-icre)9075Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

behavior/neurological

impaired behavioral response to cocaine ( J:139693 )

• mice that were previously conditioned to associate a certain cage environment with cocaine do not reinstate their cocaine-seeking behavior when administered the drug after a 16 day extinction phase

nervous system

abnormal glutamate-mediated receptor currents ( J:139693 )

• basal glutamate-mediated receptor currents are enhanced in these mice

• cocaine-induced synaptic strengthening in the ventral tegmental area does not occur in these mice

abnormal AMPA-mediated synaptic currents ( J:139693 )

• a higher occurrence of spontaneous AMPA excitatory postsynaptic currents occurs in the ventral tegmental area

abnormal NMDA-mediated synaptic currents ( J:139693 )

• NMDA-mediated excitatory postsynaptic currents are completely abolished in the ventral tegmental area of these mice

Genotype
MGI:3612448

cn4

• Allelic Composition: Grin1^tm1Rsp/Grin1^tm1Rsp; Tg(Gnrh1-cre)1Rsp/0
• Genetic Background: involves: C57BL/6

nervous system

absence of NMDA-mediated synaptic currents ( J:103818 )

• the slowly activating and deactivating NMDA component of the response after glutamate application, and the response to NMDA application are absent, however the fast-activating and -inactivating AMPA component is present

reproductive system

reproductive system phenotype ( J:103818 )

N • fertility is normal