Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myh9tm5Rsad mutation
(1 available);
any
Myh9 mutation
(219 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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renal/urinary system
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• with loss of filtration slits
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nck1tm1Paw mutation
(2 available);
any
Nck1 mutation
(40 available)
Nck2tm3Paw mutation
(0 available);
any
Nck2 mutation
(29 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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growth/size/body
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• by 4 days of age, mutants are smaller than wild-type littermates; this is more apparent by 3 weeks of age
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homeostasis/metabolism
renal/urinary system
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• at 3.5 and 4.5 weeks of age, mutants show excessive amounts of albumin in their urine, indicating damage to the filtration barrier
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• by 3.5 weeks of age, loss of podocytes is observed
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• at E16.5, differentiated foot processes are absent in mutant embryos
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• in 4-day old mutants, there is complete fusion of foot processes around the capillary loops of the glomeruli
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• by 3.5 weeks of age, focal sclerosis and other defects are observed in the glomeruli
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• by 3.5 weeks of age, focal sclerosis is observed in the glomeruli
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• mutants have a buildup of proteinaceous material in the kidney tubules
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• damaged glomerular filtration barrier
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tcf21tm1Jrt mutation
(0 available);
any
Tcf21 mutation
(14 available)
Tcf21tm2.1Seq mutation
(0 available);
any
Tcf21 mutation
(14 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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renal/urinary system
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• significantly increased urinary protein to creatinine ratio in affected mice
• streptozotocin induced diabetes at 17 weeks of age in protected cohort results in increased urinary protein to creatinine ratio
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• massive proteinuria in 40% of mice by 5 weeks of age
• 60& of mice do not develop proteinuria through 8 months of age
• in protected cohort of mice proteinuria development resumes after 32 weeks of age
• streptozotocin induced diabetes at 17 weeks of age in protected cohort results in massive proteinuria
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• dynamic proliferation of cells in Bowman's capsule
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• occasional podocyte vacuolation
• focal flattening and disorganized foot processes in all mice
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• extensive effacement in affected mice with large bumpy subepithelial protrusions of the glomerular basement membrane
• prominent fibrinogen depositions
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• simplified glomerular structure at birth and at 3 weeks of age
• total cell number reduced by 15%
• non-podocyte cell number reduced 25%
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• focal and segmental glomerulosclerosis with occasional crescent formation and podocyte vacuolation
• streptozotocin induced diabetes at 17 weeks of age in protected cohort results in dramatic glomerulosclerosis
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• with an irregular surface in affected mice
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• end stage renal disease in some mice with death at 3-6 weeks of age
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grb2tm3.1Paw mutation
(1 available);
any
Grb2 mutation
(42 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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renal/urinary system
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N |
• mice exhibit normal podocyte development and kidney function
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grb2tm1Paw mutation
(0 available);
any
Grb2 mutation
(42 available)
Grb2tm3.1Paw mutation
(1 available);
any
Grb2 mutation
(42 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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renal/urinary system
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N |
• mice exhibit normal podocyte development and kidney function
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Coro2btm1Cya mutation
(0 available);
any
Coro2b mutation
(69 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mtortm1.1Seq mutation
(0 available);
any
Mtor mutation
(115 available)
Mtortm1.2Seq mutation
(0 available);
any
Mtor mutation
(115 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
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growth/size/body
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• growth restriction observed starting around 4 weeks of age
• no overt phenotypes until after 3 weeks of age
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homeostasis/metabolism
renal/urinary system
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• protein/creatinine ratio is significantly increased at 3 weeks of age
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• distended Bowman's space at 5 weeks
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• numerous vacuolated podocytes at 4 weeks of age
• widespread vacuolation of podocytes at 5 weeks of age
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• focal effacement at 3 weeks of age but endothelium and glomerular basement membranes are preserved
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• areas of sclerosis at 4 weeks of age
• complete destruction of glomerular tuft at 5 weeks of age
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• proteinaceous casts found in tubules of 2-3 week old mice
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cellular
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• autophagosomal vesicles accumulate in podocytes beginning at 2 weeks of age
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy mutation
(5 available);
any
Gt(ROSA)26Sor mutation
(942 available)
Tg(Nphs2-cre)1Seq mutation
(0 available)
Tg(tetO-Vegfa)90Ala mutation
(0 available)
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homeostasis/metabolism
renal/urinary system
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• administration of doxycycline in the drinking water to 4 month old mice and consequent VEGF-A164 expression in podocyte of glomerulus caused proteinuria after 7 days of treatment
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Analysis Tools