Phenotypes associated with this allele

• Allele Symbol: Pparg^tm1Lja
• Allele Name: targeted mutation 1, J Larry Jameson
• Allele ID: MGI:3580102
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pparg^tm1Lja/Pparg^tm1Lja	involves: 129S1/Sv * 129X1/SvJ	MGI:3580110
ht2	Pparg^tm1Lja/Pparg^+	involves: 129S1/Sv * 129X1/SvJ	MGI:3580111

Genotype
MGI:3580110

hm1

• Allelic Composition: Pparg^tm1Lja/Pparg^tm1Lja
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Embryonic lethality of Pparg^tm1Lja/Pparg^tm1Lja mice

mortality/aging

embryonic lethality, complete penetrance ( J:98786 )

• die by E10.5

embryo

decreased embryo size ( J:98786 )

• smaller at E10.5-11.5

growth/size/body

decreased embryo size ( J:98786 )

• smaller at E10.5-11.5

Genotype
MGI:3580111

ht2

• Allelic Composition: Pparg^tm1Lja/Pparg⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

Hepatic steatosis in Pparg^tm1Lja/Pparg⁺ mice

adipose tissue

decreased body fat mass ( J:98786 )

• reduced fat mass

abnormal brown fat cell morphology ( J:98786 )

• brown adipocytes contained large multiloculated fat vacuoles not present in wild-type

decreased white fat cell size ( J:98786 )

• epididymal white adipose tissue contained a heterogeneous population of adipocytes with mostly small and medium sized cells, compared to the homozygous population of large adipocytes in wild-type

abnormal epididymal fat pad morphology ( J:98786 )

• reduced epididymal white adipose tissue depots

lipodystrophy ( J:98786 )

• exhibited lipodystrophy

growth/size/body

decreased body fat mass ( J:98786 )

• reduced fat mass

decreased body weight ( J:98786 )

• heterozygous mutants gained less weight on a standard or high fat diet than wild-type after 3 months of age, however no differences in food intake or oxygen consumption

homeostasis/metabolism

increased circulating insulin level ( J:98786 )

• males on a high-fat diet exhibited basal hyperinsulinemia

increased circulating free fatty acids level ( J:98786 )

• exhibited higher serum free fatty acid levels on a standard diet than wild-type

insulin resistance ( J:98786 )

• males on a high-fat diet exhibited insulin resistance, as evidenced by lower glucose infusion rates and impaired glucose uptake in skeletal muscle during clamp studies

decreased circulating adiponectin level ( J:98786 )

• significantly lower levels of serum adiponectin levels than in wild-type on either a standard or high fat diet

increased liver triglyceride level ( J:98786 )

• hepatic triglyceride levels were 1.8-fold greater on a standard diet and 1.46-fold greater on a high-fat diet compared to wild-type

muscle

decreased skeletal muscle cell glucose uptake ( J:98786 )

• males on a high-fat diet display impaired glucose uptake in skeletal muscle during clamp studies

liver/biliary system

increased liver triglyceride level ( J:98786 )

• hepatic triglyceride levels were 1.8-fold greater on a standard diet and 1.46-fold greater on a high-fat diet compared to wild-type

macrovesicular hepatic steatosis ( J:98786 )

• 5 month old males exhibited macrovesicular fatty changes in midzonal and centrilobular locations

microvesicular hepatic steatosis ( J:98786 )

• 5 month old males exhibited microvesicular fatty changes around the central vein

cardiovascular system

hypertension ( J:98786 )

• females, but not males, were overtly hypertensive on either the standard or high-fat diet

cellular

decreased skeletal muscle cell glucose uptake ( J:98786 )

• males on a high-fat diet display impaired glucose uptake in skeletal muscle during clamp studies

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
abdominal obesity-metabolic syndrome		DOID:0060611	OMIM:PS605552	J:98786