Phenotypes associated with this allele

• Allele Symbol: Tirap^tm1Medz
• Allele Name: targeted mutation 1, Ruslan Medzhltov
• Allele ID: MGI:3573942
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tirap^tm1Medz/Tirap^tm1Medz	involves: 129S1/Sv	MGI:3574658
hm2	Tirap^tm1Medz/Tirap^tm1Medz	involves: 129S1/Sv * C57BL/6	MGI:3574401

Genotype
MGI:3574658

hm1

• Allelic Composition: Tirap^tm1Medz/Tirap^tm1Medz
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:96018 )

• died more frequently than wildtype after S. aureus infection

immune system

abnormal macrophage physiology ( J:96018 )

• peritoneal macrophages showed only a residual response with a reduction of TNF production when stimulated with lipoteichoic acid (LTA) or macrophage-activating lipopeptide 2 (MALP-2) from Mycoplasma pneumoniae

increased susceptibility to bacterial infection ( J:96018 )

• increased susceptibility to S. aureus infection

cardiovascular system

abnormal response to cardiac infarction ( J:119941 )

• improved recovery of left ventricular function during reperfusion following ischemia

homeostasis/metabolism

abnormal response to cardiac infarction ( J:119941 )

• improved recovery of left ventricular function during reperfusion following ischemia

hematopoietic system

abnormal macrophage physiology ( J:96018 )

• peritoneal macrophages showed only a residual response with a reduction of TNF production when stimulated with lipoteichoic acid (LTA) or macrophage-activating lipopeptide 2 (MALP-2) from Mycoplasma pneumoniae

Genotype
MGI:3574401

hm2

• Allelic Composition: Tirap^tm1Medz/Tirap^tm1Medz
• Genetic Background: involves: 129S1/Sv * C57BL/6

immune system

decreased B cell proliferation ( J:96773 )

• splenocytes showed a defect in proliferation in response to the TLR4 ligand lipopolysaccharide (LPS) and ligand bacterial lipopeptide (BLP) that signals through the TLR1-TLR2 heterodimer but not to the TLR9 ligand unmethylated CpG DNA (CpG) or to the TLR7 ligand resiquimod (R-848)

abnormal macrophage physiology ( J:96773 )

• production of IL-6, IL-12 and TNF-alpha by bone marrow derived macrophages was significantly reduced in response to LPS and BLP, but not CpG

• activation of NF-kappaB, JNK, p38 and ERK in bone marrow derived macrophages was delayed in response to LPS and BLP but not CpG

abnormal dendritic cell physiology ( J:96773 )

• production of IL-6, IL-12 and TNF-alpha by dendritic cells was significantly reduced in response to LPS and BLP, but not CpG or R-848, however dendritic cells were able to mature in response to LPS and CpG

• dendritic cells stimulated with LPS activated NF-kappaB with delayed kinetics

hematopoietic system

decreased B cell proliferation ( J:96773 )

• splenocytes showed a defect in proliferation in response to the TLR4 ligand lipopolysaccharide (LPS) and ligand bacterial lipopeptide (BLP) that signals through the TLR1-TLR2 heterodimer but not to the TLR9 ligand unmethylated CpG DNA (CpG) or to the TLR7 ligand resiquimod (R-848)

abnormal macrophage physiology ( J:96773 )

• production of IL-6, IL-12 and TNF-alpha by bone marrow derived macrophages was significantly reduced in response to LPS and BLP, but not CpG

• activation of NF-kappaB, JNK, p38 and ERK in bone marrow derived macrophages was delayed in response to LPS and BLP but not CpG

cellular

decreased B cell proliferation ( J:96773 )

• splenocytes showed a defect in proliferation in response to the TLR4 ligand lipopolysaccharide (LPS) and ligand bacterial lipopeptide (BLP) that signals through the TLR1-TLR2 heterodimer but not to the TLR9 ligand unmethylated CpG DNA (CpG) or to the TLR7 ligand resiquimod (R-848)