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Allele Symbol Allele Name Allele ID |
Nod2tm1Flv targeted mutation 1, Richard A Flavell MGI:3529594 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in response to peptidoglycan or muramyl dipeptide (MDP), mice fail to develop arthritis unlike similarly treated wild-type mice
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• in response to peptidoglycan or muramyl dipeptide (MDP), mice fail to develop arthritis unlike similarly treated wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• when infected with Sanger 476, a methicillin-susceptible S. aureus strain, or MW2, a methicillin-resistant S. aureus strain
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• the phagocytic index is significantly lower in macrophage
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• in an in vitro assay the neutrophils from wild-type control mice have a higher number of internalized S. aureus
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• in the blood of mice at 6, 12, and 18 hours post infection with S.aureus
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• in the peritoneal fluid at 12 and 18 hours post infection with S.aureus
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• increase in the levels of Th1 derived cytokines
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• after S. aureus infection
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• after S. aureus infection
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• after S. aureus infection
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• bacterial loads are significantly higher at 18 hours in the kidney, peritoneal fluid, blood and mesenteric lymph nodes after bacterial infection
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• when infected with Sanger 476, a methicillin-susceptible S. aureus strain, or MW2, a methicillin-resistant S. aureus strain
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• the phagocytic index is significantly lower in macrophage
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• in an in vitro assay the neutrophils from wild-type control mice have a higher number of internalized S. aureus
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• in the blood of mice at 6, 12, and 18 hours post infection with S.aureus
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• in the peritoneal fluid at 12 and 18 hours post infection with S.aureus
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• increase in the levels of Th1 derived cytokines
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• after S. aureus infection
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• after S. aureus infection
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• after S. aureus infection
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• the phagocytic index is significantly lower in macrophage
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• in an in vitro assay the neutrophils from wild-type control mice have a higher number of internalized S. aureus
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• decreased antigen specific Ig and IgG1 production are seen following stimulation with bacterial muramyl dipeptide and HSA; however, production is normal when stimulated by HAS plus an artificial TLR7 ligand
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• the synergistic effect of bacterial muramyl dipeptide and TLR ligands on the production of IL6 and IL12 is absent in mutant macrophages
(J:96035)
• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes
(J:132126)
• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated
(J:132126)
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• bone marrow derived macrophages secrete 73% the amount of IL-6 as wild-type controls due in response to culturing with L. monocytogenes
• a similar relative reduction is observed when macrophages are pre-treated with LPS prior to L. monocytogenes incubation
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• bone marrow derived macrophages secrete 77% the amount of TNF as wild-type controls in response to culturing with L. monocytogenes
• TNF secretion is 57% that of controls when macrophages are pre-treated with LPS prior to L. monocytogenes incubation
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• homozygotes are resistant to endotoxin shock produced by LPS following bacterial muramyl dipeptide priming but not to endotoxin shock produced by LPS alone
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• homozygotes are more susceptible to Listeria monocytogenes infection via intragastric exposure but not via intraperitoneal or intravenous exposure
• however, no signs of intestinal inflammation and no difference in susceptibility to dextran sulfate induced colitis are seen
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• decreased antigen specific Ig and IgG1 production are seen following stimulation with bacterial muramyl dipeptide and HSA; however, production is normal when stimulated by HAS plus an artificial TLR7 ligand
|
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• the synergistic effect of bacterial muramyl dipeptide and TLR ligands on the production of IL6 and IL12 is absent in mutant macrophages
(J:96035)
• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes
(J:132126)
• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated
(J:132126)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes
• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated
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• bone marrow derived macrophages secrete 40% the amount of IL-6 as wild-type controls due in response to culturing with L. monocytogenes
• IL-6 secretion is 7% that of controls when macrophages are pre-treated with LPS prior to L. monocytogenes incubation
• IL-6 secretion is 18% that of controls when macrophages are pre-treated with E. Coli prior to L. monocytogenes incubation
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• bone marrow derived macrophages secrete 56% the amount of TNF as wild-type controls in response to culturing with L. monocytogenes
• TNF secretion is 13% that of controls when macrophages are pre-treated with LPS prior to L. monocytogenes incubation
• TNF secretion is 26% that of controls when macrophages are pre-treated with E. Coli prior to L. monocytogenes incubation
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• there is an approximately 5-fold higher number of bacterial colony-forming units in the liver and spleen than in wild-type mice 2 days after infection with L. monocytogenes
• when mice are pre-treated with LPS before infection, bacterial load in the liver and spleen is about 50- to 70- fold higher than in wild-type mice
• mutant mice that are pretreated with LPS before L. monocytogenes infection have a survival rate of about 35% compared to controls that have a 100% survival rate
• these LPS pre-treated mice have a higher number of liver microabcesses than controls after L. monocytogenes infection that were LPS pre-treated
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• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes
• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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