Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Egln1tm2.1Fsl mutation
(0 available);
any
Egln1 mutation
(21 available)
Epas1tm1Mcs mutation
(1 available);
any
Epas1 mutation
(64 available)
Gt(ROSA)26Sortm9(cre/ESR1)Arte mutation
(2 available);
any
Gt(ROSA)26Sor mutation
(944 available)
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hematopoietic system
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N |
• hematocrit level, erythropoietin level and spleen architecture and weight are similar to mice without cre/ESR1 allele
• the Epas1 allele functions to rescue the erythrocytosis phenotype
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mortality/aging
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• survival is improved relative to mice carrying the Egln1 null allele, but not to control levels
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skeleton
hematopoietic system
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N |
• mice exhibit normal frequencies of KLS cells (hematopoietic stem cells and multipotential progenitors) numbers of red blood cells and lymphocytes and hematocrit
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1tm1Mcs mutation
(1 available);
any
Epas1 mutation
(64 available)
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc mutation
(2 available)
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hematopoietic system
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• decreased frequency of CD71+/Ter119+ cells in the bone marrow
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hematopoietic system
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N |
• mice exhibit normal hematopoietic stem cell frequency
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• decreased frequency of CD71+/Ter119+ cells in the bone marrow
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skeleton
hematopoietic system
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N |
• mice exhibit normal hematocrit
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Egln1tm1.1Brei mutation
(0 available);
any
Egln1 mutation
(21 available)
Epas1tm1Mcs mutation
(1 available);
any
Epas1 mutation
(64 available)
Tg(CD68-icre)1Bwlx mutation
(0 available)
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mortality/aging
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N |
• no premature death is observed
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hematopoietic system
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N |
• erythropoiesis phenotype seen in Phd2 single mutants is absent from double mutants
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• HCT is lower than wild type
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• lower than in wild-type
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1tm1Mcs mutation
(1 available);
any
Epas1 mutation
(64 available)
Tg(CD68-icre)1Bwlx mutation
(0 available)
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mortality/aging
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N |
• no premature death observed
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1tm1.1Mcs mutation
(0 available);
any
Epas1 mutation
(64 available)
Epas1tm1Mcs mutation
(1 available);
any
Epas1 mutation
(64 available)
Tg(CAG-cre/Esr1*)5Amc mutation
(9 available)
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cardiovascular system
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N |
• tamoxifen-treated mice exhibit normal carotid body morphology
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• likely secondary to anemia in tamoxifen-treated
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• impaired responsiveness to hypoxia with reduced secretory rate in tamoxifen-treated mice
• lower shifted quantal charge and reduced mean charge per event in tamoxifen-treated mice exposed to mild stimuli
• reduced increase in NADH and mitochondrial function induced by hypoxia in tamoxifen-treated mice
• however, tamoxifen-treated mice exhibit normal hypercapnia and depolarization with high extracellular potassium ion, and normal secretion induced by CO2 and high potassium ion
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hematopoietic system
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• in tamoxifen-treated mice
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• in tamoxifen-treated mice
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homeostasis/metabolism
growth/size/body
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• likely secondary to anemia in tamoxifen-treated
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muscle
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• likely secondary to anemia in tamoxifen-treated
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nervous system
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• impaired responsiveness to hypoxia with reduced secretory rate in tamoxifen-treated mice
• lower shifted quantal charge and reduced mean charge per event in tamoxifen-treated mice exposed to mild stimuli
• reduced increase in NADH and mitochondrial function induced by hypoxia in tamoxifen-treated mice
• however, tamoxifen-treated mice exhibit normal hypercapnia and depolarization with high extracellular potassium ion, and normal secretion induced by CO2 and high potassium ion
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Epas1tm1.1Mcs mutation
(0 available);
any
Epas1 mutation
(64 available)
Epas1tm1Mcs mutation
(1 available);
any
Epas1 mutation
(64 available)
Ndor1Tg(UBC-cre/ERT2)1Ejb mutation
(6 available);
any
Ndor1 mutation
(32 available)
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hematopoietic system
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• spleens are enlarged relative to controls (0.5% spleen weight to body weight vs. ~0.4% in controls)
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• occurs after postnatal cre induction
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• erythroid progenitors from bone marrow form ~50% fewer erythroid burst-forming units (BFU-E) and colony-forming units (CFU-E) in culture than control cells, whereas erythroid progenitors from the spleen form more BFU-E and CFU-E
• there are fewer CD71+ immature erythroid progenitors in the bone marrow, and a higher percentage of CD71+ /Ter119+ double positive cells in the spleen
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• with acute deletion of Epas1 at 6-8 weeks induced by tamoxifen administration, animals have decreased red blood cell numbers relative to controls
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• with acute deletion of Epas1 at 6-8 weeks induced by tamoxifen administration, animals have decreased hematocrit relative to controls
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• with acute deletion of Epas1 at 6-8 weeks induced by tamoxifen administration, animals have decreased hemoglobin levels relative to controls
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• numbers are decreased compared to controls
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• mice show weak induction of erythropoietin after phenylhydrazine treatment
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immune system
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• spleens are enlarged relative to controls (0.5% spleen weight to body weight vs. ~0.4% in controls)
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growth/size/body
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• spleens are enlarged relative to controls (0.5% spleen weight to body weight vs. ~0.4% in controls)
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Analysis Tools