Phenotypes associated with this allele

• Allele Symbol: Ep300^tm2Pkb
• Allele Name: targeted mutation 2, Paul K Brindle
• Allele ID: MGI:3526150
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ep300^tm2Pkb/Ep300^tm2Pkb Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3618539
cn2	Crebbp^tm1Jvd/Crebbp^tm1Jvd Ep300^tm2Pkb/Ep300^tm2Pkb Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3618581
cn3	Ep300^tm2Pkb/Ep300^tm2Pkb Tg(MMTV-cre)4Mam/0	involves: 129P2/OlaHsd * FVB	MGI:3618540

Genotype
MGI:3618539

cn1

• Allelic Composition: Ep300^tm2Pkb/Ep300^tm2Pkb; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

increased double-positive T cell number ( J:105505 )

• increased 3 fold

decreased single-positive T cell number ( J:105505 )

• half the normal numbers of single positive thymocyte

• proportion of CD4+ to CD8+ is normal in spleen and peripheral blood

hematopoietic system

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

increased double-positive T cell number ( J:105505 )

• increased 3 fold

decreased single-positive T cell number ( J:105505 )

• half the normal numbers of single positive thymocyte

• proportion of CD4+ to CD8+ is normal in spleen and peripheral blood

endocrine/exocrine glands

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

Genotype
MGI:3618581

cn2

• Allelic Composition: Crebbp^tm1Jvd/Crebbp^tm1Jvd; Ep300^tm2Pkb/Ep300^tm2Pkb; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

abnormal T cell number ( J:105505 )

• drop in number of single positive thymocytes comparable to the increase in double negative cells

• no increase in CD8+ single positive thymocytes

• near absence of single positive thymocytes in the periphery

increased double-negative T cell number ( J:105505 )

• 10 fold increase in the percentage of double negative thymocytes

decreased double-positive T cell number ( J:105505 )

• near absence in the periphery

hematopoietic system

abnormal T cell number ( J:105505 )

• near absence of single positive thymocytes in the periphery

• drop in number of single positive thymocytes comparable to the increase in double negative cells

• no increase in CD8+ single positive thymocytes

increased double-negative T cell number ( J:105505 )

• 10 fold increase in the percentage of double negative thymocytes

decreased double-positive T cell number ( J:105505 )

• near absence in the periphery

Genotype
MGI:3618540

cn3

• Allelic Composition: Ep300^tm2Pkb/Ep300^tm2Pkb; Tg(MMTV-cre)4Mam/0
• Genetic Background: involves: 129P2/OlaHsd * FVB

neoplasm

neoplasm ( J:105505 )

N • unexpectedly, no lymphomas developed for at least the first year of life