Phenotypes associated with this allele
Allelic
Composition |
Ido1tm1Alm/Ido1tm1Alm
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Genetic
Background |
either: (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * CBA) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ido1tm1Alm mutation
(2 available);
any
Ido1 mutation
(30 available)
|
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reproductive system
|
N |
• no effect on reproductive success
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ido1tm1Alm mutation
(2 available);
any
Ido1 mutation
(30 available)
|
|
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ido1tm1Alm mutation
(2 available);
any
Ido1 mutation
(30 available)
|
|
|
reproductive system
|
N |
• males exhibit normal fertility and testis weight
|
 |
• increase in the proportion of spermatozoa exhibiting abnormal morphology, including pinhead, giant head, hairpin and kite spermatozoa
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• whereas wild-type caput epididymis exhibits a high KYN/Trp ratio indicating that it is in an immunosuppressed state, mutants have a much lower KYN/Trp ratio, indicating that the caput epididymis is in an inflammatory state
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• caudal epididymal sperm counts are nearly twice as high in mutants as in wild-type males
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• levels of some cytokines are higher in mutant caput epididymis
• significantly greater amounts of protein in caput epididymis extracts than in controls
• lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues
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cellular
|
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• increase in the proportion of spermatozoa exhibiting abnormal morphology, including pinhead, giant head, hairpin and kite spermatozoa
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• lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues as indicated by an increase in multilamellar/multivesicular bodies
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hematopoietic system
|
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• decrease in leukocyte count in caudal epididymal fluid
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homeostasis/metabolism
immune system
|
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• decrease in leukocyte count in caudal epididymal fluid
|
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• levels of some cytokines are higher in mutant caput epididymis
|
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• whereas wild-type caput epididymis exhibits a high KYN/Trp ratio indicating that it is in an immunosuppressed state, mutants have a much lower KYN/Trp ratio, indicating that the caput epididymis is in an inflammatory state
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ido1tm1Alm mutation
(2 available);
any
Ido1 mutation
(30 available)
|
|
|
immune system
|
|
• CD19+ dendritic cells fail to express IFNalpha following B7 ligation
|
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• after CTLA4-Ig treatment, no spleen cells show expression of IFNalpha
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ido1tm1Alm mutation
(2 available);
any
Ido1 mutation
(30 available)
|
|
|
cellular
|
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• peritoneal macrophages show disrupted mitochondrial morphology
|
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• peritoneal macrophages show reduced numbers of mitochondria
|
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• marker analysis indicates increased mitophagy in peritoneal macrophages
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• peritoneal macrophage mitochondria show a deficit only in complex I activity
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hematopoietic system
|
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• peritoneal macrophages show disrupted mitochondrial morphology and reduced numbers of mitochondria
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• primary mouse peritoneal macrophages exhibit reduced cellular NAD+ amounts and suppressed oxygen consumption while having increased glycolysis, thus disrupting macrophage respiration
• supplementation of peritoneal macrophages with kynurenine restores NAD+ levels and normalizes oxygen consumption and extracellular acidification rate
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homeostasis/metabolism
immune system
|
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• peritoneal macrophages show disrupted mitochondrial morphology and reduced numbers of mitochondria
|
|
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• primary mouse peritoneal macrophages exhibit reduced cellular NAD+ amounts and suppressed oxygen consumption while having increased glycolysis, thus disrupting macrophage respiration
• supplementation of peritoneal macrophages with kynurenine restores NAD+ levels and normalizes oxygen consumption and extracellular acidification rate
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ido1tm1Alm mutation
(2 available);
any
Ido1 mutation
(30 available)
|
|
|
immune system
|
|
• reduced ear swelling in a contact hypersensitivity assay with oxazolone
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• in a contact hypersensitivity assay with oxazolone
• however, lymph node cells treated with PMA and ionomycin exhibit normal secretion
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• in a contact hypersensitivity assay with oxazolone
• however, lymph node cells treated with PMA and ionomycin exhibit normal secretion
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• in a contact hypersensitivity assay with oxazolone
• however, lymph node cells treated with PMA and ionomycin exhibit normal secretion
|
Analysis Tools