Analysis Tools
immune system
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• 25% of splenocytes survive four days of culture while all of controls die
• cultured splenocytes die off at a rate about half that of controls
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• immortalized immature mast cells can be cultured out of spleens with a frequency of 3 x 10-5, which is at least 1000-fold more frequent than controls
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• immortalized monocytes can be cultured of out of spleens unlike with wild-type controls
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• 2 of 9 mice have increased numbers of thymocytes
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• spleens have 1.6-fold higher number of cells and are visibly larger
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• ratio of myeloid to B cell precursors is 2.2:1 versus 1.3:1 in wild-type bone marrow
• however, there is a 5-fold increase in the number of B cell precursors found in bone marrow culture compared to controls
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• mice rarely have expansion of the white pulp
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• myeloid cells present in splenocyte culture die-off at a slower rate than controls
• including IL-3 in the culture greatly enhanced survival of transgenic myeloid cells but not non-transgenic myeloid cells
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• B cells present in splenocyte culture die-off at about half the rate as wild-type B cells do
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• T cells are still present after 3 days of splenocyte culture compared to no T cells surviving culture of wild-type splenocytes
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• bone marrow derived macrophages have significantly less apoptosis than controls 20 hours after infection with pneumococcal infection
• by 48 hours after infection, apoptosis levels are similar to that in wild-type controls suggesting pneumococcal-associated apoptosis is delayed rather than suppressed
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• pneumococcal-associated macrophage apoptosis is lower by about half in these mice 14 hours after infection
• mice fail to clear bacteria after infection with 104 CFU compared to controls
• all transgenic mice with more than 102 CFU in the lung develope bacteremia, which does not occur in nontransgenic mice
• at higher infection doses, the amount of CFUs in the lung is a log unit higher than that in wild-type mice
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hematopoietic system
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• 25% of splenocytes survive four days of culture while all of controls die
• cultured splenocytes die off at a rate about half that of controls
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• immortalized immature mast cells can be cultured out of spleens with a frequency of 3 x 10-5, which is at least 1000-fold more frequent than controls
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• immortalized monocytes can be cultured of out of spleens unlike with wild-type controls
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• 2 of 9 mice have increased numbers of thymocytes
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• spleens have 1.6-fold higher number of cells and are visibly larger
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• there is a doubling in the number of myeloid progenitors found in the bone marrow
(J:50604)
• these progenitors exhibit enhanced survival in the absence of growth factors
(J:50604)
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• ratio of myeloid to B cell precursors is 2.2:1 versus 1.3:1 in wild-type bone marrow
• however, there is a 5-fold increase in the number of B cell precursors found in bone marrow culture compared to controls
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• extramedullary hematopoiesis is very active in these mice
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• there is a doubling in the number of erythroid progenitors found in the bone marrow
• these progenitors exhibit enhanced survival in the absence of growth factors
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• mice rarely have expansion of the white pulp
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• myeloid cells present in splenocyte culture die-off at a slower rate than controls
• including IL-3 in the culture greatly enhanced survival of transgenic myeloid cells but not non-transgenic myeloid cells
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• B cells present in splenocyte culture die-off at about half the rate as wild-type B cells do
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• T cells are still present after 3 days of splenocyte culture compared to no T cells surviving culture of wild-type splenocytes
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• bone marrow derived macrophages have significantly less apoptosis than controls 20 hours after infection with pneumococcal infection
• by 48 hours after infection, apoptosis levels are similar to that in wild-type controls suggesting pneumococcal-associated apoptosis is delayed rather than suppressed
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neoplasm
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• about 27% of mice at 6-11 months of age develop lymphomas of various subtypes
• lymphomas develop in about 50% of transgenic mice at 18 to 23 months of age and in about 65% of mice at 2 years of age compared to 12% of non-transgenic mice by 2 years of age
• two-thirds of the cases involve disseminated disease affecting lymph nodes (mesenteric, renal, mediastinal, and cervical areas) and other tissues (liver, lung, kidney)
• in the remaining one third of affected transgenic animals, disease was localized to mesenteric lymph nodes or presented as extra-nodal lymphoma in the gastrointestinal tract
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• non-disseminated T cell lymphoma occurred in 1 case out of 18 total lymphoma cases
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• the majority of lymphomas are diffuse large-cell lymphoma with B-cell origins or indeterminate origin
• some cases present a polymorphic cytology consisting of a heterogeneous mixture of small and large lymphocytes
• B cells lacking IgM expression constitute the majority of the large cells in the cases of disseminated disease
• Ig-heavy chain analysis of lymphomas reveals clonal rearrangement of the immunoglobulin heavy-chain locus (except in the one case of T cell lymphoma) demonstrating a B cell origin
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cellular
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• B cells present in splenocyte culture die-off at about half the rate as wild-type B cells do
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• T cells are still present after 3 days of splenocyte culture compared to no T cells surviving culture of wild-type splenocytes
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• bone marrow derived macrophages have significantly less apoptosis than controls 20 hours after infection with pneumococcal infection
• by 48 hours after infection, apoptosis levels are similar to that in wild-type controls suggesting pneumococcal-associated apoptosis is delayed rather than suppressed
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• 25% of splenocytes survive four days of culture while all of controls die
• cultured splenocytes die off at a rate about half that of controls
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• immortalized immature mast cells can be cultured out of spleens with a frequency of 3 x 10-5, which is at least 1000-fold more frequent than controls
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• immortalized monocytes can be cultured of out of spleens unlike with wild-type controls
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endocrine/exocrine glands
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• 2 of 9 mice have increased numbers of thymocytes
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• non-disseminated T cell lymphoma occurred in 1 case out of 18 total lymphoma cases
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growth/size/body
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• spleens have 1.6-fold higher number of cells and are visibly larger
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