Phenotypes associated with this allele

• Allele Symbol: Tg(Pou5f1-EGFP)2Mnn
• Allele Name: transgene insertion 2, Jeff Mann
• Allele ID: MGI:3057158
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj/0 Tg(Pou5f1-EGFP)2Mnn/0	involves: C57BL/6 * C57BL/6J * CBA/CaJ	MGI:7516821

Genotype
MGI:7516821

cx1

• Allelic Composition: Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj/0; Tg(Pou5f1-EGFP)2Mnn/0
• Genetic Background: involves: C57BL/6 * C57BL/6J * CBA/CaJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal primordial germ cell morphology ( J:338852 )

• expression analysis indicates loss of germ cell fate following doxycycline treatment

decreased primordial germ cell number ( J:338852 )

• following doxycycline treatment at E7.5, primordial germ cell (PGC) numbers are slightly reduced at E9.5

• following doxycycline treatment at E7.5, by E10.5 very few PGCs are found in the gut tube and genital ridges

abnormal primordial germ cell migration ( J:338852 )

• following doxycycline treatment at E7.5, primordial germ cells are motionless in time lapsed images between E9.5 and E10.5

decreased primordial germ cell proliferation ( J:338852 )

• following doxycycline treatment at E7.5, PGCs from E10.5 embryos show a 2-fold increase in the percentage of cells in the G1 phase and marked reduction in the number of cells in the S phase

• when PCGs are cultured in the presence of doxycycline, cell proliferation declines at 72h post treatment

cellular

abnormal primordial germ cell morphology ( J:338852 )

• expression analysis indicates loss of germ cell fate following doxycycline treatment

decreased primordial germ cell number ( J:338852 )

• following doxycycline treatment at E7.5, primordial germ cell (PGC) numbers are slightly reduced at E9.5

• following doxycycline treatment at E7.5, by E10.5 very few PGCs are found in the gut tube and genital ridges

abnormal primordial germ cell migration ( J:338852 )

• following doxycycline treatment at E7.5, primordial germ cells are motionless in time lapsed images between E9.5 and E10.5

decreased primordial germ cell proliferation ( J:338852 )

• following doxycycline treatment at E7.5, PGCs from E10.5 embryos show a 2-fold increase in the percentage of cells in the G1 phase and marked reduction in the number of cells in the S phase

• when PCGs are cultured in the presence of doxycycline, cell proliferation declines at 72h post treatment