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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Pou5f1-EGFP)2Mnn
transgene insertion 2, Jeff Mann
MGI:3057158
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Gcnatm1.1Dcp/Y
Dppa3tm1(cre)Peli/Dppa3+
Tg(Pou5f1-EGFP)2Mnn/0 		involves: 129S7/SvEvBrd * C57BL/6J * C57BL/6N * CBA/CaJ MGI:8250243
cx2
 		Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj/0
Tg(Pou5f1-EGFP)2Mnn/0 		involves: C57BL/6 * C57BL/6J * CBA/CaJ MGI:7516821


Genotype
MGI:8250243
cn1
Allelic
Composition		 Gcnatm1.1Dcp/Y
Dppa3tm1(cre)Peli/Dppa3+
Tg(Pou5f1-EGFP)2Mnn/0
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6J * C57BL/6N * CBA/CaJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dppa3tm1(cre)Peli mutation (0 available); any Dppa3 mutation (24 available)
Gcnatm1.1Dcp mutation (1 available); any Gcna mutation (1 available)
Tg(Pou5f1-EGFP)2Mnn mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:344952 )
N
• at E12.5, male mice show no differences in primordial germ cell (PGC) numbers relative to wild-type males, suggesting that the observed age-dependent loss of germ cells may not have an embryonic origin




Genotype
MGI:7516821
cx2
Allelic
Composition		 Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj/0
Tg(Pou5f1-EGFP)2Mnn/0
Genetic
Background		 involves: C57BL/6 * C57BL/6J * CBA/CaJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj mutation (0 available)
Tg(Pou5f1-EGFP)2Mnn mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal primordial germ cell morphology ( J:338852 )
• expression analysis indicates loss of germ cell fate following doxycycline treatment
decreased primordial germ cell number ( J:338852 )
• following doxycycline treatment at E7.5, primordial germ cell (PGC) numbers are slightly reduced at E9.5
• following doxycycline treatment at E7.5, by E10.5 very few PGCs are found in the gut tube and genital ridges
abnormal primordial germ cell migration ( J:338852 )
• following doxycycline treatment at E7.5, primordial germ cells are motionless in time lapsed images between E9.5 and E10.5
decreased primordial germ cell proliferation ( J:338852 )
• following doxycycline treatment at E7.5, PGCs from E10.5 embryos show a 2-fold increase in the percentage of cells in the G1 phase and marked reduction in the number of cells in the S phase
• when PCGs are cultured in the presence of doxycycline, cell proliferation declines at 72h post treatment

cellular
abnormal primordial germ cell morphology ( J:338852 )
• expression analysis indicates loss of germ cell fate following doxycycline treatment
decreased primordial germ cell number ( J:338852 )
• following doxycycline treatment at E7.5, primordial germ cell (PGC) numbers are slightly reduced at E9.5
• following doxycycline treatment at E7.5, by E10.5 very few PGCs are found in the gut tube and genital ridges
abnormal primordial germ cell migration ( J:338852 )
• following doxycycline treatment at E7.5, primordial germ cells are motionless in time lapsed images between E9.5 and E10.5
decreased primordial germ cell proliferation ( J:338852 )
• following doxycycline treatment at E7.5, PGCs from E10.5 embryos show a 2-fold increase in the percentage of cells in the G1 phase and marked reduction in the number of cells in the S phase
• when PCGs are cultured in the presence of doxycycline, cell proliferation declines at 72h post treatment




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Send questions and comments to User Support. 		last database update
09/30/2025
MGI 6.24 		The Jackson Laboratory