Phenotypes associated with this allele

• Allele Symbol: Tg(CD3E)26Cpt
• Allele Name: transgene insertion 26, Cox Terhorst
• Allele ID: MGI:3052629
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Cd3e^tm1Cpt/Cd3e^tm1Cpt Tg(CD3E)26Cpt/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3850637
tg2	Tg(CD3E)26Cpt/Tg(CD3E)26Cpt	involves: C57BL/6J * CBA/J	MGI:3798917

Genotype
MGI:3850637

cx1

• Allelic Composition: Cd3e^tm1Cpt/Cd3e^tm1Cpt; Tg(CD3E)26Cpt/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thymus hypoplasia ( J:54756 )

• thymic cellularity is half of normal

decreased T cell number ( J:54756 )

• T cell numbers are reduced by about 60%

decreased double-positive T cell number ( J:54756 )

• double-positive thymocyte numbers are only about 17% of total thymocytes compared to 85% in wild-type controls

hematopoietic system

thymus hypoplasia ( J:54756 )

• thymic cellularity is half of normal

decreased T cell number ( J:54756 )

• T cell numbers are reduced by about 60%

decreased double-positive T cell number ( J:54756 )

• double-positive thymocyte numbers are only about 17% of total thymocytes compared to 85% in wild-type controls

endocrine/exocrine glands

thymus hypoplasia ( J:54756 )

• thymic cellularity is half of normal

Genotype
MGI:3798917

tg2

• Allelic Composition: Tg(CD3E)26Cpt/Tg(CD3E)26Cpt
• Genetic Background: involves: C57BL/6J * CBA/J

mortality/aging

decreased survivor rate ( J:134589 )

• only 40% of mice survive until weaning

neonatal lethality, incomplete penetrance ( J:134589 )

• 11% of mice die within 24 hours of birth

postnatal lethality ( J:134589 )

• 29% of mice die before weaning

growth/size/body

decreased body weight ( J:134589 )

• mice weigh 29% less than age- and sex- matched controls at three weeks of age and 27% less than controls at 7 weeks of age

immune system

decreased uterine NK cell number ( J:134589 )

♀ • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls

♀ • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta

abnormal thymus corticomedullary boundary morphology ( J:81711 )

• no clear distinction exists between the cortex and the medulla

thymus hypoplasia ( J:54756 )

• thymic cellularity is half of normal

arrested T cell differentiation ( J:81711 )

• very few thymocytes are present with the vast majority of them arrested in the double negative stage

absent NK cells ( J:81711 )

• NK cells are absent in these mice based upon the lack of splenocytes expressing LGL-1, NK1.1, and "bright" levels of AGM-1

• the NK target cell line YAC-1 is not lysed when co-cultured with activated splenocytes also indicating an absence of NK cells

decreased T cell number ( J:54756 )

• T cell numbers are reduced by about 40%

decreased thymocyte number ( J:81711 )

• mean thymocyte number in mice up to 6 months of age is decreased 70-fold compared to controls

decreased double-positive T cell number ( J:54756 )

• absolute numbers (but not relative percentage) of double-positive thymocytes is reduced by half

reproductive system

abnormal uterus morphology ( J:134589 )

♀ • the uterine arterioles undergo abnormal histological changes during the pregnancy of homozygote transgenic females mated with homozygotic transgenic males

♀ • at 10 days of gestation, the endothelial cells become cuboidal with thickening of the arteriolar media and adventitia occurring

♀ • at 12 days of gestation, the endothelium becomes flat and deposition of lipid and foam cells occurs

♀ • at 14 days of gestation, loss of vessel integrity occurs with a discontinous endothelium and leakage of blood

♀ • similar histological changes are observed during the first 12 days of gestation that result from breeding homozygote transgenic females with wild-type CD-1 males

decreased uterine NK cell number ( J:134589 )

♀ • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls

♀ • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta

abnormal endometrium morphology ( J:134589 )

♀ • the metrial gland and uterine NK cells are absent from the uteri of pregnant mice at day 14 of gestation

absent endometrial glands ( J:134589 )

♀ • the metrial gland is absent from the uteri of pregnant mice at day 14 of gestation

reduced fertility ( J:134589 )

• fetal viability is low with 67% of embryos being in the late stages of resorption at E14

• the loss of fetuses starts at 10 days post-conception

decreased litter size ( J:134589 )

• matings of homozygous mice produce on average 6 pups while control mice produce 9 pus

embryo

abnormal placenta morphology ( J:134589 )

• placenta morphology is abnormal in homozygote transgenic females mated with wild-type CD-1 males

• at 12 days of gestation, the placenta becomes more disorganized with variation in appearances between implantation sites and trophoblast cells moving adjacent to decidual artioles

decreased uterine NK cell number ( J:134589 )

♀ • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls

♀ • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta

abnormal placenta labyrinth morphology ( J:134589 )

• placenta labyrinth morphology is abnormal in homozygote transgenic females mated with wild-type CD-1 males

• extremely large syncytia are present with peripheral nuclei surrounding a vacuolated cytoplasm

• extensive smooth muscle development is sometimes found adjacent to the labyrinthine trophoblast

small placenta ( J:134589 )

• the placenta of mice 14 days after gestation is 45% the size of control CBA mice

• reductions of placenta size are evident at 10 days of gestation

hematopoietic system

decreased uterine NK cell number ( J:134589 )

♀ • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls

♀ • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta

abnormal thymus corticomedullary boundary morphology ( J:81711 )

• no clear distinction exists between the cortex and the medulla

thymus hypoplasia ( J:54756 )

• thymic cellularity is half of normal

arrested T cell differentiation ( J:81711 )

• very few thymocytes are present with the vast majority of them arrested in the double negative stage

absent NK cells ( J:81711 )

• NK cells are absent in these mice based upon the lack of splenocytes expressing LGL-1, NK1.1, and "bright" levels of AGM-1

• the NK target cell line YAC-1 is not lysed when co-cultured with activated splenocytes also indicating an absence of NK cells

decreased T cell number ( J:54756 )

• T cell numbers are reduced by about 40%

decreased thymocyte number ( J:81711 )

• mean thymocyte number in mice up to 6 months of age is decreased 70-fold compared to controls

decreased double-positive T cell number ( J:54756 )

• absolute numbers (but not relative percentage) of double-positive thymocytes is reduced by half

endocrine/exocrine glands

decreased uterine NK cell number ( J:134589 )

♀ • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls

♀ • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta

abnormal thymus corticomedullary boundary morphology ( J:81711 )

• no clear distinction exists between the cortex and the medulla

thymus hypoplasia ( J:54756 )

• thymic cellularity is half of normal

decreased thymocyte number ( J:81711 )

• mean thymocyte number in mice up to 6 months of age is decreased 70-fold compared to controls

absent endometrial glands ( J:134589 )

♀ • the metrial gland is absent from the uteri of pregnant mice at day 14 of gestation