Phenotypes associated with this allele

• Allele Symbol: Birc5^tm1Mak
• Allele Name: targeted mutation 1, Tak Mak
• Allele ID: MGI:3046203
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Birc5^tm1Mak/Birc5^tm1Mak	involves: 129P2/OlaHsd	MGI:3046206
cn2	Birc5^tm1Mak/Birc5^tm1Mak Tg(Lck-cre)I57Jxm/0	involves: 129P2/OlaHsd	MGI:3046207

Genotype
MGI:3046206

hm1

• Allelic Composition: Birc5^tm1Mak/Birc5^tm1Mak
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:90892 )

• homozygous mice are fertile and display no abnormalities

Genotype
MGI:3046207

cn2

• Allelic Composition: Birc5^tm1Mak/Birc5^tm1Mak; Tg(Lck-cre)I57Jxm/0
• Genetic Background: involves: 129P2/OlaHsd

cellular

abnormal cell cycle ( J:90892 )

• more CD25⁺CD44^- double negative cells are in G1 and fewer are in S indicating cell cycle arrest

• no typical telophase cells can be found among the Birc5 deficient cells

increased T cell apoptosis ( J:90892 )

• 37% CD25⁺CD44^-- and 32% of CD25^-CD44^- double negative cells are apoptotic compared to only 5-7% of cells in littermate controls

hematopoietic system

increased T cell apoptosis ( J:90892 )

• 37% CD25⁺CD44^-- and 32% of CD25^-CD44^- double negative cells are apoptotic compared to only 5-7% of cells in littermate controls

abnormal thymus corticomedullary boundary morphology ( J:90892 )

• the thymus lacks the normal cortex-medulla compartmentalization

small thymus ( J:90892 )

• the thymus is much smaller

abnormal T cell morphology ( J:90892 )

• shorter and thicker spindles are seen in mutant CD25⁺CD44^-- and CD25^-CD44^- double negative cells

• the vast majority of the cells in the thymus appear to be immature thymocytes

decreased thymocyte number ( J:90892 )

• the average number of thymocytes is about 2% that seen in littermate controls

decreased double-negative T cell number ( J:90892 )

• the overall number of DN cells is decreased however these now make up about 95% of the total thymocytes

• CD25⁺CD44^- DN cells make up about 80% of the total DN cells compared to less than 50% in littermate controls

• CD25^-CD44^- DN cells are reduced to about 4% of the total DN cells compared to less than about 30% in littermate controls

immune system

increased T cell apoptosis ( J:90892 )

• 37% CD25⁺CD44^-- and 32% of CD25^-CD44^- double negative cells are apoptotic compared to only 5-7% of cells in littermate controls

abnormal thymus corticomedullary boundary morphology ( J:90892 )

• the thymus lacks the normal cortex-medulla compartmentalization

small thymus ( J:90892 )

• the thymus is much smaller

abnormal T cell morphology ( J:90892 )

• shorter and thicker spindles are seen in mutant CD25⁺CD44^-- and CD25^-CD44^- double negative cells

• the vast majority of the cells in the thymus appear to be immature thymocytes

decreased thymocyte number ( J:90892 )

• the average number of thymocytes is about 2% that seen in littermate controls

decreased double-negative T cell number ( J:90892 )

• the overall number of DN cells is decreased however these now make up about 95% of the total thymocytes

• CD25⁺CD44^- DN cells make up about 80% of the total DN cells compared to less than 50% in littermate controls

• CD25^-CD44^- DN cells are reduced to about 4% of the total DN cells compared to less than about 30% in littermate controls

endocrine/exocrine glands

abnormal thymus corticomedullary boundary morphology ( J:90892 )

• the thymus lacks the normal cortex-medulla compartmentalization

small thymus ( J:90892 )

• the thymus is much smaller

decreased thymocyte number ( J:90892 )

• the average number of thymocytes is about 2% that seen in littermate controls