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Allele Symbol Allele Name Allele ID |
Tg(Tcra/Tcrb)1Vbo transgene insertion 1, Harald von Boehmer MGI:2680176 |
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| Summary |
7 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• autoimmune diabetes developed with accelerated kinetics
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| type 1 diabetes mellitus | DOID:9744 |
OMIM:222100 |
J:85985 | |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice develop arthritis more quickly but with disease penetrance and severity similar to double Tg(Tcra/Tcrb)1Vbo/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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• mice develop arthritis more quickly but with disease penetrance and severity similar to double Tg(Tcra/Tcrb)1Vbo/0 Tg(H2-Ea-HA)#Ajca/0 transgenic mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 signaling is needed to maintain this regulatory T subset
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• the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to transgenic mice that have the Il2ra locus intact, indicating that IL2 signalling is not needed to generate this regulatory T cell subset
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• CD25-positive CD4 T cells from the thymus have a reduced capacity to suppress T cell proliferation in vitro
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• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 signaling is needed to maintain this regulatory T subset
|
|
• the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to transgenic mice that have the Il2ra locus intact, indicating that IL2 signalling is not needed to generate this regulatory T cell subset
|
|
• CD25-positive CD4 T cells from the thymus have a reduced capacity to suppress T cell proliferation in vitro
|
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 is needed to maintain this regulatory T subset
|
|
• in the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to mice that express the same transgenes but have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
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• in the thymus, CD25-positive CD4 T cells express high levels of Foxp3
• this phenotype in the thymus is similar to transgenic mice that have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
• only 0.5% of CD25-positive CD4 T cells in the lymph nodes and spleen express Foxp3 compared to 9% of these cells in transgenic mice with the Il2 locus intact
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• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 is needed to maintain this regulatory T subset
|
|
• in the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to mice that express the same transgenes but have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
|
|
• in the thymus, CD25-positive CD4 T cells express high levels of Foxp3
• this phenotype in the thymus is similar to transgenic mice that have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
• only 0.5% of CD25-positive CD4 T cells in the lymph nodes and spleen express Foxp3 compared to 9% of these cells in transgenic mice with the Il2 locus intact
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mild inflammatory processes are seen in the hearts of some arthritic mice
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• severe deletion of HA-specific 6.5+CD4+CD8- single-positive thymocytes indicating deletion of autoreactive CD4+ T cells, although a subset of 6.5+CD4+ T cells evades deletion and accumulates in spleens and lymph nodes
• modest increase in the percentages of CD4+CD8- Foxp3+ thymocytes and of CD4+Foxp3+ splenocytes, however, the numbers of these cells that express the clonotypic TS1 TCR are reduced, reflecting severe deletion of clonotype-expressing thymocytes
• increase in frequency of IL-17-secreting CD4+ T cells in the joint-draining lymph nodes and spleens of arthritic mice
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• arthritic mice exhibit a higher level of serum IgG than control Tg(Tcra/Tcrb)1Vbo/0 mice
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• proinflammatory cytokine levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• interleukin-6 levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• swollen joints show a high degree of synovitis
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• majority of mice spontaneously develop inflammatory arthritis, showing overt joint inflammation and swelling affecting both front and rear paws
• joint inflammation first becomes evident between 6 and 8 weeks of age, and by 14 weeks almost all mice show at least one inflamed paw
• penetrance of arthritis is similar in males and females
• inflammatory arthritis develops in mice despite substantial deletion of autoreactive CD4+ T cells and despite the formation of Foxp3+ Tregs
• treatment of prearthritic mice with anti-TNF antibody results in a reduction in arthritis penetrance associated with a reduced accumulation of Th17 cells in the joints but has no effect on CD4+Foxp3+ Tregs
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• mild inflammatory processes are seen in the kidneys of some arthritic mice
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• severe deletion of HA-specific 6.5+CD4+CD8- single-positive thymocytes indicating deletion of autoreactive CD4+ T cells, although a subset of 6.5+CD4+ T cells evades deletion and accumulates in spleens and lymph nodes
• modest increase in the percentages of CD4+CD8- Foxp3+ thymocytes and of CD4+Foxp3+ splenocytes, however, the numbers of these cells that express the clonotypic TS1 TCR are reduced, reflecting severe deletion of clonotype-expressing thymocytes
• increase in frequency of IL-17-secreting CD4+ T cells in the joint-draining lymph nodes and spleens of arthritic mice
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• arthritic mice exhibit a higher level of serum IgG than control Tg(Tcra/Tcrb)1Vbo/0 mice
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• proinflammatory cytokine levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• interleukin-6 levels are increased in serum of arthritic mice compared to single Tg(Tcra/Tcrb)1Vbo/0 mice
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• mild inflammatory processes are seen in the hearts of some arthritic mice
|
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• mild inflammatory processes are seen in the kidneys of some arthritic mice
|
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• extensive perivascular infiltrates in the lungs of arthritic mice
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• swollen joints show a high degree of synovitis
|
|
• majority of mice spontaneously develop inflammatory arthritis, showing overt joint inflammation and swelling affecting both front and rear paws
• joint inflammation first becomes evident between 6 and 8 weeks of age, and by 14 weeks almost all mice show at least one inflamed paw
• penetrance of arthritis is similar in males and females
• inflammatory arthritis develops in mice despite substantial deletion of autoreactive CD4+ T cells and despite the formation of Foxp3+ Tregs
• treatment of prearthritic mice with anti-TNF antibody results in a reduction in arthritis penetrance associated with a reduced accumulation of Th17 cells in the joints but has no effect on CD4+Foxp3+ Tregs
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• swollen joints show a high degree of articular degeneration
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• there is a substantial increase in the frequencies and absolute numbers of CD25-positive CD4 T cells both in the thymus and peripheral lymph nodes compared to mice that express only the TCR transgene
|
|
• CD25-positive CD4 T cells from the thymus express higher levels of Foxp3 compared to mice that express only the TCR transgene
|
|
• there is a substantial increase in the frequencies and absolute numbers of CD25-positive CD4 T cells both in the thymus and peripheral lymph nodes compared to mice that express only the TCR transgene
|
|
• CD25-positive CD4 T cells from the thymus express higher levels of Foxp3 compared to mice that express only the TCR transgene
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the percentage of double-positive T cells is increased compared to mice with just the TCR transgene (71% vs 62%)
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• the percentage of CD4 T cells in the thymus is decreased compared to mice with just the TCR transgene (20% vs 31%)
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• 5% of CD4 T cells from lymph nodes are CD25-positive and suppress the proliferation of nave CD4 T cells to hemagglutinin antigen in both in vitro and in vivo assays
• 10% of CD4-positive CD8-negative T cells from the thymus express CD25 and suppress the in vitro proliferation of nave CD4 T cells to hemagglutinin antigen
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• the percentage of double-positive T cells is increased compared to mice with just the TCR transgene (71% vs 62%)
|
|
• the percentage of CD4 T cells in the thymus is decreased compared to mice with just the TCR transgene (20% vs 31%)
|
|
• 5% of CD4 T cells from lymph nodes are CD25-positive and suppress the proliferation of nave CD4 T cells to hemagglutinin antigen in both in vitro and in vivo assays
• 10% of CD4-positive CD8-negative T cells from the thymus express CD25 and suppress the in vitro proliferation of nave CD4 T cells to hemagglutinin antigen
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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