Phenotypes associated with this allele

• Allele Symbol: Lipe^tm1Land
• Allele Name: targeted mutation 1, Dominique Langin
• Allele ID: MGI:2677463
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lipe^tm1Land/Lipe^tm1Land	involves: 129	MGI:3783539
hm2	Lipe^tm1Land/Lipe^tm1Land	involves: 129 * C57BL/6J	MGI:2677468

Genotype
MGI:3783539

hm1

• Allelic Composition: Lipe^tm1Land/Lipe^tm1Land
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased skeletal muscle glycogen level ( J:106148 )

• after 10 hours of ex vivo incubation, soleus muscles exhibit a 40% decrease in glycogen concentration compared to concentration after 2 hours unlike wild-type mice

abnormal lipid homeostasis ( J:106148 )

• after 10 hours of ex vivo incubation, soleus muscles exhibit a decrease in mobilization of intramuscular lipid deposits unlike in wild-type mice

muscle

decreased skeletal muscle glycogen level ( J:106148 )

• after 10 hours of ex vivo incubation, soleus muscles exhibit a 40% decrease in glycogen concentration compared to concentration after 2 hours unlike wild-type mice

Genotype
MGI:2677468

hm2

• Allelic Composition: Lipe^tm1Land/Lipe^tm1Land
• Genetic Background: involves: 129 * C57BL/6J

reproductive system

oligozoospermia ( J:85540 )

♂

male infertility ( J:85540 )

♂

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:133126 )

hyperglycemia ( J:85540 )

• slight hyperglycemia in a fasted, not a fed, state

• associated with hyperinsulinemia

increased circulating insulin level ( J:85540 )

• insulin levels are increased in both the fasted and fed state

decreased circulating leptin level ( J:133126 )

• when fed a high fat diet, mice fail to exhibit an increase in leptin levels as in wild-type mice

increased oxygen consumption ( J:133126 )

• when fed a high fat diet, oxygen consumption in white adipose tissue is increased almost 3-fold

insulin resistance ( J:85540 )

• moderate impairment of insulin sensitivity

• impaired insulin sensitivity is observed in skeletal muscle and adipose cells

decreased circulating adiponectin level ( J:133126 )

• when fed a standard or high fat diet

abnormal lipolysis ( J:85540 )

• adipocytes exhibit a blunted catecholamine-stimulated glycerol release and a reduced release of free fatty acids

• white adipocyte exhibit an accumulation of diglycerides

• however, lipase activity in skeletal muscle and liver is normal

adipose tissue

decreased subcutaneous adipose tissue amount ( J:133126 )

• when fed a high fat diet, mice possess virtually no subcutaneous fat deposits

decreased white adipose tissue amount ( J:133126 )

• when fed a high fat diet

abnormal white fat cell morphology ( J:133126 )

• when fed a high fat diet, mitochondrial size in adipocytes of white adipose tissue is increased

abnormal adipose tissue physiology ( J:85540 )

• insulin stimulated lipogenesis is impaired

digestive/alimentary system

abnormal feces composition ( J:133126 )

• when fed a high fat diet, mice exhibit decreased lipid content in their feces compared to wild-type mice

behavior/neurological

increased food intake ( J:133126 )

• after 5 months on a high fat diet, caloric intake per total body weight is increased 40% compared to in wild-type mice

growth/size/body

decreased body weight ( J:133126 )

• when fed a high fat diet

decreased susceptibility to diet-induced obesity ( J:133126 )

muscle

decreased muscle cell glucose uptake ( J:85540 )

• insulin-stimulated glucose uptake in skeletal muscle is decreased 17% compared to in wild-type mice

endocrine/exocrine glands

increased pancreatic beta cell mass ( J:85540 )

• morphometric analysis of pancreata showed a doubling of beta cell mass

pancreatic islet hyperplasia ( J:85540 )

integument

decreased subcutaneous adipose tissue amount ( J:133126 )

• when fed a high fat diet, mice possess virtually no subcutaneous fat deposits

cellular

oligozoospermia ( J:85540 )

♂

decreased muscle cell glucose uptake ( J:85540 )

• insulin-stimulated glucose uptake in skeletal muscle is decreased 17% compared to in wild-type mice