Phenotypes associated with this allele

• Allele Symbol: Nr6a1^tm3Coo
• Allele Name: targeted mutation 3, Austin J Cooney
• Allele ID: MGI:2674000
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nr6a1^tm3Coo/Nr6a1^tm3Coo Tg(Zp3-cre)93Knw/0	involves: 129S7/SvEvBrd * C57BL/6	MGI:2674001

Genotype
MGI:2674001

cn1

• Allelic Composition: Nr6a1^tm3Coo/Nr6a1^tm3Coo; Tg(Zp3-cre)93Knw/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal oocyte morphology ( J:85054 )

♀ • double-oocyte follicles may be derived from a single oocyte, as two nucleus-like structures have been noted in one oocyte

polyovular ovarian follicle ( J:85054 )

♀ • 6 of 7 mutant females at diestrus display 1 to 3 follicles containing double oocytes

♀ • double-oocyte follicles are observed at the primary, secondary, and antral stages

♀ • however, ovarian histology is otherwise normal, and no differences in ovary weight or size are observed

abnormal diestrus ( J:85054 )

♀ • the expression levels of two oocyte-specific genes, BMP15 and GDF9, are increased >2-fold in mutant ovaries at diestrus (but not at estrus or metestrus) relative to those in control ovaries

prolonged diestrus ( J:85054 )

♀ • the diestrus phase of the estrous cycle is significantly prolonged

prolonged estrous cycle ( J:85054 )

♀ • the average length of the estrous cycle is much longer than that of control littermates, as a result of a prolonged diestrus phase

♀ • no differences are observed at the other stages of the estrous cycle

reduced female fertility ( J:85054 )

♀ • female mutants are subfertile, showing a ~50% reduction in the number of litters per month relative to control littermates

decreased litter size ( J:85054 )

• at day 5 after birth, the number of pups per litter is significantly lower than that of control littermates

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:85054 )

N • at 2-3 months of age, mutant females exhibit:

N • normal cicrulating levels of FSH, LH, testosterone and progesterone at proestrus and estrus

N • normal estradiol levels at estrus

N • normal LH levels at diestrus

decreased circulating testosterone level ( J:85054 )

• at diestrus, mutant females display significantly reduced circulating testosterone levels relative control littermates

decreased circulating estradiol level ( J:85054 )

• at diestrus, mutant females display significantly reduced circulating estradiol levels relative control littermates

decreased circulating follicle stimulating hormone level ( J:85054 )

• at diestrus, mutant females display significantly reduced circulating FSH levels relative control littermates

decreased circulating progesterone level ( J:85054 )

• at diestrus, mutant females display significantly reduced circulating progesterone levels relative control littermates

endocrine/exocrine glands

polyovular ovarian follicle ( J:85054 )

♀ • 6 of 7 mutant females at diestrus display 1 to 3 follicles containing double oocytes

♀ • double-oocyte follicles are observed at the primary, secondary, and antral stages

♀ • however, ovarian histology is otherwise normal, and no differences in ovary weight or size are observed

cellular

abnormal oocyte morphology ( J:85054 )

♀ • double-oocyte follicles may be derived from a single oocyte, as two nucleus-like structures have been noted in one oocyte