Phenotypes associated with this allele

• Allele Symbol: Plcd1^tm1Tta
• Allele Name: targeted mutation 1, Tadaomi Takenawa
• Allele ID: MGI:2667234
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plcd1^tm1Tta/Plcd1^tm1Tta	involves: 129P2/OlaHsd	MGI:5547368
hm2	Plcd1^tm1Tta/Plcd1^tm1Tta	involves: 129P2/OlaHsd * C57BL/6J	MGI:2670617
cx3	Plcd1^tm1Tta/olt	involves: 129P2/OlaHsd * C3H/HeJOrl * C57BL/6	MGI:3823245

Genotype
MGI:5547368

hm1

• Allelic Composition: Plcd1^tm1Tta/Plcd1^tm1Tta
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal granulocyte differentiation ( J:205633 )

• enhanced proliferation of immature granulocytes

enlarged spleen ( J:205633 )

• mild

decreased B cell number ( J:205633 )

• in the bone marrow

increased leukocyte cell number ( J:205633 )

• greater than 2-fold increase in peripheral blood leukocytes

increased granulocyte number ( J:205633 )

• not affected by antibiotic treatment

• in peripheral blood, spleen and bone marrow

increased circulating interleukin-17 level ( J:205633 )

• however, epidermal expression using a transgene is sufficient for normal IL17 levels

enlarged lymph nodes ( J:205633 )

• in the inguinal and axillary lymph nodes

• not affected by antibiotic treatment

• however, mesenteric lymph nodes exhibit normal weight

enlarged axillary lymph nodes ( J:205633 )

• increased weight

enlarged inguinal lymph nodes ( J:205633 )

• increased weight

increased interleukin-17 secretion ( J:205633 )

• 3.9-fold increase in IL17+ cells in the inguinal, but not mesenteric, lymph nodes

homeostasis/metabolism

increased circulating interleukin-17 level ( J:205633 )

• however, epidermal expression using a transgene is sufficient for normal IL17 levels

cellular

abnormal granulocyte differentiation ( J:205633 )

• enhanced proliferation of immature granulocytes

hematopoietic system

hematopoietic system phenotype ( J:205633 )

N • bone marrow transplanted into recipient mice fails to induce granulocytosis or lymphadenopathy

abnormal granulocyte differentiation ( J:205633 )

• enhanced proliferation of immature granulocytes

enlarged spleen ( J:205633 )

• mild

abnormal common myeloid progenitor cell morphology ( J:205633 )

• increased myeloid progenitor cells in the bone marrow

decreased erythrocyte cell number ( J:205633 )

• in the bone marrow

decreased B cell number ( J:205633 )

• in the bone marrow

increased leukocyte cell number ( J:205633 )

• greater than 2-fold increase in peripheral blood leukocytes

increased granulocyte number ( J:205633 )

• in peripheral blood, spleen and bone marrow

• not affected by antibiotic treatment

growth/size/body

enlarged spleen ( J:205633 )

• mild

Genotype
MGI:2670617

hm2

• Allelic Composition: Plcd1^tm1Tta/Plcd1^tm1Tta
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

neoplasm

increased skin tumor incidence ( J:83948 )

• skin tumors

• spontaneous tumors developed in the ventral skin of about 20% ofhomozygotes over 3 months of age

• arose below the interfollicular epithelium and contained large cysts and sebocyte-like cells surrounded by basaloid keratinocytes

endocrine/exocrine glands

increased sebocyte number ( J:83948 )

• greatly increased numbers of sebocytes

growth/size/body

epidermal cyst ( J:83948 )

• hair follicle derived cysts in dermis filled with horny cells and epithelium

• cysts enlarge around 25 days

decreased body size ( J:83948 )

• runting was evident

integument

abnormal keratinocyte differentiation ( J:83948 )

• the number of interfollicular and upper-follicular cells that incorporate BrdU was increased 8X

• upper regions of hair follicles differentiated like interfollicular epidermis

increased sebocyte number ( J:83948 )

• greatly increased numbers of sebocytes

epidermal cyst ( J:83948 )

• hair follicle derived cysts in dermis filled with horny cells and epithelium

• cysts enlarge around 25 days

alopecia ( J:83948 )

• hair loss observed 8 days after birth

abnormal hair follicle morphology ( J:83948 )

• normal number of hair follicles but many fail to penetrate epidermis and became zigzagged

• follicles occluded by differentiated keratinocytes

• follicle morphology severely disrupted from 15 to 25 days of age

abnormal epidermis suprabasal layer morphology ( J:83948 )

• the number of suprabasal cell layers was increased by one or two layers

• becomes less noticeable by 15 days of age

increased skin tumor incidence ( J:83948 )

• skin tumors

• spontaneous tumors developed in the ventral skin of about 20% ofhomozygotes over 3 months of age

• arose below the interfollicular epithelium and contained large cysts and sebocyte-like cells surrounded by basaloid keratinocytes

cellular

abnormal keratinocyte differentiation ( J:83948 )

• the number of interfollicular and upper-follicular cells that incorporate BrdU was increased 8X

• upper regions of hair follicles differentiated like interfollicular epidermis

Genotype
MGI:3823245

cx3

• Allelic Composition: Plcd1^tm1Tta/olt
• Genetic Background: involves: 129P2/OlaHsd * C3H/HeJOrl * C57BL/6

reproductive system

reproductive system phenotype ( J:142517 )

N • male fertility is normal

integument

alopecia ( J:142517 )

abnormal epidermal layer morphology ( J:142517 )

• increased cell layers in regions with more pronounced alopecia