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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Myh11-cre,-EGFP)2Mik
transgene insertion 2, Michael I Kotlikoff
MGI:2653286
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Mirc32tm1.1Jtm/Mirc32tm1.1Jtm
Tg(Myh11-cre,-EGFP)2Mik/? 		B6.Cg-Mirc32tm1.1Jtm Tg(Myh11-cre,-EGFP)2Mik MGI:5707654
cn2
 		Wwtr1tm1.2Hmc/Wwtr1tm1.2Hmc
Yap1tm1.2Hmc/Yap1+
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: 129 * C57BL/6 * DBA/2 MGI:7861681
cn3
 		Gipc1tm1.1Mhsi/Gipc1tm1.1Mhsi
Tg(Myh11-cre,-EGFP)2Mik/? 		involves: 129S1/SvImJ * 129S4/SvJaeSor * C57BL/6 * DBA/2 MGI:5696325
cn4
 		Smarca2tm1Mya/Smarca2tm1Mya
Smarca4tm1.2Pcn/Smarca4tm1.1Pcn
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: 129S2/SvPas * C57BL/6 * DBA/2 MGI:5009697
cn5
 		Smarca2tm1Mya/Smarca2+
Smarca4tm1.2Pcn/Smarca4tm1.1Pcn
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: 129S2/SvPas * C57BL/6 * DBA/2 MGI:5009696
cn6
 		Smarca4tm1.2Pcn/Smarca4tm1.1Pcn
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: 129S2/SvPas * C57BL/6 * DBA/2 MGI:5009694
cn7
 		Mylktm1.1Bph/Mylktm1.2Bph
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: C57BL/6 * C57BL/6N * DBA/2 MGI:5563581
cn8
 		Mylktm1.1Bph/Mylktm1.1Bph
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: C57BL/6 * C57BL/6N * DBA/2 MGI:5563580
cn9
 		Foxf1tm2Rhc/Foxf1+
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: C57BL/6 * DBA/2 MGI:5550502
cn10
 		Foxf1tm2Rhc/Foxf1tm2Rhc
Tg(Myh11-cre,-EGFP)2Mik/0 		involves: C57BL/6 * DBA/2 MGI:5550501


Genotype
MGI:5707654
cn1
Allelic
Composition		 Mirc32tm1.1Jtm/Mirc32tm1.1Jtm
Tg(Myh11-cre,-EGFP)2Mik/?
Genetic
Background		 B6.Cg-Mirc32tm1.1Jtm Tg(Myh11-cre,-EGFP)2Mik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mirc32tm1.1Jtm mutation (1 available); any Mirc32 mutation (3 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal mucosa morphology ( J:214382 )
• lamina propia myofibroblasts within the ulcers of DSS treated (+ 2 days recovery) mice are abnormal
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration
intestinal ulcer ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
abnormal colon morphology ( J:214382 )
• colons from DSS treated (+ 2 days recovery) mice remain hemorrhagic and necrotic
• however, phenotype is less severe than in Mirc32tm1.2Jtm mice
colonic necrosis ( J:214382 )
• observed in DSS treated (+ 2 days recovery) mice
impaired intestine regeneration ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration
• pH3+ cells are reduced in number
increased susceptibility to induced colitis ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration

endocrine/exocrine glands
abnormal large intestine crypts of Lieberkuhn morphology ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration

immune system
increased susceptibility to induced colitis ( J:214382 )
• DSS treated (+ 2 days recovery) mice exhibit impaired epithelial regeneration




Genotype
MGI:7861681
cn2
Allelic
Composition		 Wwtr1tm1.2Hmc/Wwtr1tm1.2Hmc
Yap1tm1.2Hmc/Yap1+
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: 129 * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
Wwtr1tm1.2Hmc mutation (2 available); any Wwtr1 mutation (23 available)
Yap1tm1.2Hmc mutation (1 available); any Yap1 mutation (40 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal mitochondrial physiology ( J:344215 )
• aortic vascular smooth muscle (VSM) mitochondria show significantly lower maximal oxygen consumption and spare respiratory capacity, similar to what is observed in mice double homozygous for Wnt16tm2a(EUCOMM)Wtsi and Ldlrtm1Her




Genotype
MGI:5696325
cn3
Allelic
Composition		 Gipc1tm1.1Mhsi/Gipc1tm1.1Mhsi
Tg(Myh11-cre,-EGFP)2Mik/?
Genetic
Background		 involves: 129S1/SvImJ * 129S4/SvJaeSor * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gipc1tm1.1Mhsi mutation (1 available); any Gipc1 mutation (42 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
cardiovascular system phenotype ( J:221554 )
N
• normal arterial tree branching and collateral artery number in the heart and kidney
• normal spinotrapezius arterial tree
• blood flow recovery after hindlimb ischemia is similar to controls at 2 weeks
abnormal systemic artery morphology ( J:221554 )
• trend toward fewer small arteries in heart and kidney
• trend toward larger arterial size in hind limbs




Genotype
MGI:5009697
cn4
Allelic
Composition		 Smarca2tm1Mya/Smarca2tm1Mya
Smarca4tm1.2Pcn/Smarca4tm1.1Pcn
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smarca2tm1Mya mutation (0 available); any Smarca2 mutation (91 available)
Smarca4tm1.1Pcn mutation (0 available); any Smarca4 mutation (110 available)
Smarca4tm1.2Pcn mutation (1 available); any Smarca4 mutation (110 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, complete penetrance ( J:172661 )
• fewer than expected mice are present at P10
• no mice survives beyond 2 weeks

digestive/alimentary system
abnormal digestive system morphology ( J:172661 )
• by 7 to 10 days, all mice develop enlarged gastrointestinal tract unlike control mice
abnormal intestine morphology ( J:172661 )
• mice exhibit dilated intestines filled with air and fecal matter unlike control mice
abnormal intestinal smooth muscle morphology ( J:172661 )
• at E17.5, mice exhibit shorter small intestine compared with control mice
abnormal small intestine morphology ( J:172661 )
• at E17.5, mice exhibit shorter small intestine compared with control mice

muscle
abnormal intestinal smooth muscle morphology ( J:172661 )
• at E17.5, mice exhibit shorter small intestine compared with control mice
abnormal muscle physiology ( J:172661 )
• in neonates, smooth muscle cell apoptosis in the proximal and distal colon is increased compared to in control mice
• however, proliferation of intestinal smooth muscle cell is equivalent to in wild-type mice
impaired smooth muscle contractility ( J:172661 )
• cannulated colonic segments fail to exhibit spontaneous contractile activity compared with control tissue

renal/urinary system
abnormal urinary bladder morphology ( J:172661 )
• by 7 to 10 days, all mice exhibit enlarged urinary bladder unlike control mice




Genotype
MGI:5009696
cn5
Allelic
Composition		 Smarca2tm1Mya/Smarca2+
Smarca4tm1.2Pcn/Smarca4tm1.1Pcn
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smarca2tm1Mya mutation (0 available); any Smarca2 mutation (91 available)
Smarca4tm1.1Pcn mutation (0 available); any Smarca4 mutation (110 available)
Smarca4tm1.2Pcn mutation (1 available); any Smarca4 mutation (110 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
enlarged cecum ( J:172661 )
• by 4 weeks of age
decreased colon length ( J:172661 )
• the colon is shorter than in wild-type mice
megacolon ( J:172661 )
• by 4 weeks of age
abnormal small intestine morphology ( J:172661 )
• enlarged by 4 weeks of age
decreased small intestine length ( J:172661 )
• mice exhibit short smaller intestine




Genotype
MGI:5009694
cn6
Allelic
Composition		 Smarca4tm1.2Pcn/Smarca4tm1.1Pcn
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smarca4tm1.1Pcn mutation (0 available); any Smarca4 mutation (110 available)
Smarca4tm1.2Pcn mutation (1 available); any Smarca4 mutation (110 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:172661 )
• fewer than expected mice are present at P10 due to cardiopulmonary defects

digestive/alimentary system
abnormal intestinal smooth muscle morphology ( J:172661 )
• smooth muscle cells are disorganized in the colon compared to in wild-type mice
decreased colon length ( J:172661 )
• in neonates, the colon is shorter than in wild-type mice
abnormal small intestine morphology ( J:172661 )
• mice exhibit enlarged ileum and jejunum compared with wild-type mice
enlarged ileum ( J:172661 )
• mice exhibit enlarged ileum compared with wild-type mice
abnormal jejunum morphology ( J:172661 )
• mice exhibit enlarged jejunum compared with wild-type mice
decreased small intestine length ( J:172661 )
• in neonates, the small intestines are shorter than in wild-type mice

muscle
abnormal intestinal smooth muscle morphology ( J:172661 )
• smooth muscle cells are disorganized in the colon compared to in wild-type mice
abnormal muscle physiology ( J:172661 )
• in neonates, smooth muscle cell apoptosis in the proximal and distal colon is increased compared to in wild-type mice
impaired smooth muscle contractility ( J:172661 )
• in response to KCl or carbachol, colonic rings exhibit impaired contractility compared with wild-type tissue

cardiovascular system
patent ductus arteriosus ( J:172661 )
• in cyanotic mice
ventricular septal defect ( J:172661 )
• in cyanotic mice
dilated heart ( J:172661 )
• in 6 of 18 mice at P0 to P2
abnormal pulmonary circulation ( J:172661 )
• lungs are hyperemic with accumulation of eosinophilic lipoproteinaceous material in the alveolar air space compared to in wild-type mice

respiratory system
abnormal pulmonary circulation ( J:172661 )
• lungs are hyperemic with accumulation of eosinophilic lipoproteinaceous material in the alveolar air space compared to in wild-type mice
primary atelectasis ( J:172661 )
• in cyanotic mice

homeostasis/metabolism
cyanosis ( J:172661 )
• in 6 of 18 mice at P0 to P2

cellular
patent ductus arteriosus ( J:172661 )
• in cyanotic mice




Genotype
MGI:5563581
cn7
Allelic
Composition		 Mylktm1.1Bph/Mylktm1.2Bph
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: C57BL/6 * C57BL/6N * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mylktm1.1Bph mutation (0 available); any Mylk mutation (106 available)
Mylktm1.2Bph mutation (0 available); any Mylk mutation (106 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Shorter small intestines in mgi:5563563/mgi:5563563 mgi:2653286/0 and mgi:5563563/mgi:5563564 mgi:2653286/0 mice

mortality/aging
neonatal lethality, incomplete penetrance ( J:204964 )
• fewer than expected mice are born
prenatal lethality, incomplete penetrance ( J:204964 )
• fewer than expected mice are born

digestive/alimentary system
abnormal small intestine morphology ( J:204964 )
• shorter than in control mice

muscle
impaired smooth muscle contractility ( J:204964 )
• induced by high KCl, carbachol or ET1 in the colon
• however, the L-type calcium channel inhibitor diltiazem blocks contractile responses to high KCl

growth/size/body
growth/size/body region phenotype ( J:204964 )
N
• mice exhibit normal body weight




Genotype
MGI:5563580
cn8
Allelic
Composition		 Mylktm1.1Bph/Mylktm1.1Bph
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: C57BL/6 * C57BL/6N * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mylktm1.1Bph mutation (0 available); any Mylk mutation (106 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Shorter small intestines in mgi:5563563/mgi:5563563 mgi:2653286/0 and mgi:5563563/mgi:5563564 mgi:2653286/0 mice

mortality/aging
neonatal lethality, incomplete penetrance ( J:204964 )
• fewer than expected mice are born
prenatal lethality, incomplete penetrance ( J:204964 )
• fewer than expected mice are born

digestive/alimentary system
abnormal small intestine morphology ( J:204964 )
• shorter than in control mice

muscle
impaired smooth muscle contractility ( J:204964 )
• induced by high KCl, carbachol or ET1 in the colon
• however, the L-type calcium channel inhibitor diltiazem blocks contractile responses to high KCl

growth/size/body
growth/size/body region phenotype ( J:204964 )
N
• mice exhibit normal body weight




Genotype
MGI:5550502
cn9
Allelic
Composition		 Foxf1tm2Rhc/Foxf1+
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxf1tm2Rhc mutation (0 available); any Foxf1 mutation (14 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
lethality, incomplete penetrance ( J:203847 )
• no time point given

muscle
abnormal intestinal smooth muscle morphology ( J:203847 )
• slight decreased thickness of the circular smooth muscle layer in the colon
impaired contractility of intestinal smooth muscle ( J:203847 )
• impaired depolarization induced contractility of colonic rings

digestive/alimentary system
abnormal intestinal smooth muscle morphology ( J:203847 )
• slight decreased thickness of the circular smooth muscle layer in the colon




Genotype
MGI:5550501
cn10
Allelic
Composition		 Foxf1tm2Rhc/Foxf1tm2Rhc
Tg(Myh11-cre,-EGFP)2Mik/0
Genetic
Background		 involves: C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxf1tm2Rhc mutation (0 available); any Foxf1 mutation (14 available)
Tg(Myh11-cre,-EGFP)2Mik mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
perinatal lethality, complete penetrance ( J:203847 )
• mice die prior to or immediately after birth

digestive/alimentary system
dilated esophagus ( J:203847 )
• distended esophagus

muscle




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Send questions and comments to User Support. 		last database update
09/30/2025
MGI 6.24 		The Jackson Laboratory