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Allele Symbol Allele Name Allele ID |
Txktm1Pls targeted mutation 1, Pamela L Schwartzberg MGI:2652126 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• splenocytes stimulated with anti-CD3e or concanavalin A secrete ~50% of levels of Il-2 produced by wild-type cells
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• mice infected with Toxoplasma gondii survive the early infection which requires natural killer cells; compared to Itk-null and double-null mice, ~50% of animals die by 102 days, while >80% of wild-type mice survive over 18 weeks
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15
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• majority of single-positive CD8+ T cell in the thymus have morphological characteristics similar to memory T cells (CD25lo, CD44hi, CD69lo, CD122+, HSAlo and NK1.1int)
• 85% of peripheral CD8+ T cells are memory T cells based on surface markers
• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15
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• single-positive CD8+ T cell numbers are increased 3-fold in the thymus
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• CD8+ T cells have increased secretion of IFN-gamma upon ex vivo stimulation by PMA and ionomycin
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• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15
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• majority of single-positive CD8+ T cell in the thymus have morphological characteristics similar to memory T cells (CD25lo, CD44hi, CD69lo, CD122+, HSAlo and NK1.1int)
• 85% of peripheral CD8+ T cells are memory T cells based on surface markers
• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15
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• single-positive CD8+ T cell numbers are increased 3-fold in the thymus
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mature T cells fail to proliferate upon T cell receptor activation (by anti-CD3e or concanavalin A); proliferation defects are observed in both CD4+ and CD8+ populations
• proliferation defects are only partially overcome by exogenous Il-2
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• animals have reduced numbers of mature T cells, particularly CD4+ T cells, resulting in a decreased CD4:CD8 ratio
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• numbers of CD4+ and CD8+ T cells are increased relative to Itk-null mice; however total T cell numbers are normal
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• mice infected with Toxoplasma gondii survive the early infection which requires natural killer cells, but by 30 days post-infection, mice are visibly ill and have a mean survival time of 41 days
• after 30 days, mice have increased numbers of brain cysts indicating defect in ability to limit infection
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• mature T cells fail to proliferate upon T cell receptor activation (by anti-CD3e or concanavalin A); proliferation defects are observed in both CD4+ and CD8+ populations
• proliferation defects are only partially overcome by exogenous Il-2
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• animals have reduced numbers of mature T cells, particularly CD4+ T cells, resulting in a decreased CD4:CD8 ratio
|
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• numbers of CD4+ and CD8+ T cells are increased relative to Itk-null mice; however total T cell numbers are normal
|
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• mature T cells fail to proliferate upon T cell receptor activation (by anti-CD3e or concanavalin A); proliferation defects are observed in both CD4+ and CD8+ populations
• proliferation defects are only partially overcome by exogenous Il-2
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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