Analysis Tools|
Allele Symbol Allele Name Allele ID |
Wnt3tm1Amc targeted mutation 1, Andrew P McMahon MGI:2450902 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygotes fail to gastrulate
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• homozygotes never develop limbs
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype
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• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%
• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected
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• 22 of 44 mutant mice exhibit mild to severe autopod defects
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• mutant mice display normal forelimbs except in two cases (2/44) where digit 5 was absent or fused to digit 4
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• mutant mice display hindlimb defects of variable penetrance and expressivity, ranging from completely normal to entirely absent hindlimbs
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• 3 of 44 mutant mice show complete absence of hindlimbs
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• 17 of 44 mutant mice show extensive truncations that extend into more proximal segments of the hindlimb
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• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally
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• mutants display variable AER disruption in the distal margin of the hindlimb consistent with a variable hindlimb phenotype
|
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• at E10.5, the dorsoventral girth of the AER in the distal margin of the hindlimb is reduced to ~50%
• at E10.5, the dorsoventral girth of the forelimb AER is also reduced; however, the anterior/posterior length of the AER is generally unaffected
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• mutants display extensive apoptosis throughout the hindlimb mesenchyme and adjacent ectoderm at the 35-42 somite stage; elevated apoptosis in the mesenchyme is more significant dorsally
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at E10.5, mutant mice exhibit a reduction in the anterior/posterior length of the AER
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• mutant mice display variable forelimb defects ranging from three digits (anterior digits 1-3) and normal zeugopod (radius and ulna) to one digit (digit 2 or 3) and also zeugopod defects (missing ulna); in most cases, the humerus appears unaffected
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• at E10.5, mutant mice exhibit a reduction in the anterior/posterior length of the AER
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 03/25/2025 MGI 6.24 |
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