Phenotypes associated with this allele

• Allele Symbol: Card14⁺
• Allele Name: wild type
• Allele ID: MGI:2440316
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Card14^em9Lutzy/Card14^+	C57BL/6J-Card14^em9Lutzy/J	MGI:6314145
ht2	Card14^em1Maak/Card14^+	C57BL/6JJmsSlc-Card14^em1Maak	MGI:7861316
ht3	Card14^em2Tshu/Card14^+	involves: C57BL/6	MGI:6441186
ht4	Card14^em3Tshu/Card14^+	involves: C57BL/6	MGI:6441187
cx5	Card14^em2Tshu/Card14^+ Il17a^tm1Cdon/Il17a^tm1Cdon	involves: C57BL/6	MGI:6441189
cx6	Card14^em3Tshu/Card14^+ Il17a^tm1Cdon/Il17a^tm1Cdon	involves: C57BL/6	MGI:6441426

Genotype
MGI:6314145

ht1

• Allelic Composition: Card14^em9Lutzy/Card14⁺
• Genetic Background: C57BL/6J-Card14^em9Lutzy/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

increased keratinocyte proliferation ( J:262723 )

• hyperproliferation of keratinocytes in the basal layer

skin inflammation ( J:262723 )

• psoriatic skin contains an infiltrate of CD45+ leukocytes composed of increased numbers of neutrophils, myeloid antigen-presenting cells and gamma-delta and alpha-beta T cells

abnormal epidermal layer morphology ( J:262723 )

• epidermis shows presence of microabscesses made up of neutrophils

• however, mice do not have an epidermal barrier defect

hyperkeratosis ( J:262723 )

• ears show areas of hyperkeratosis, including parakeratosis and orthokeratosis and keratotic follicular plugging

orthokeratosis ( J:262723 )

parakeratosis ( J:262723 )

acanthosis ( J:262723 )

• ear tissues show acanthosis due to keratinocyte hyperproliferation and immune cell infiltration of the skin

thick epidermis ( J:262723 )

• tail skin shows increased thickening of the epidermis

dry skin ( J:262723 )

• mice develop dry flaky skin patches on the back at 5 days of age which begin to disappear at 7 days

flaky skin ( J:262723 )

• mice develop dry flaky skin patches on the back at 5 days of age which begin to disappear at 7 days

skin lesions ( J:262723 )

• scaling skin lesions

skin hyperplasia ( J:262723 )

• tail skin shows marked hyperplasia with increased thickening of the epidermis and immune cell infiltration

psoriasis ( J:262723 )

• mice develop thickened squamous skin on the tail between 2-3 weeks of age and the ears at 4-5 weeks of age, similar to human psoriasis skin lesions

• 100% of adult mice exhibit a chronic psoriatic phenotype with thickened squamous skin of the ears and tail and dry skin around the eyes and whiskers

• tail skin shows marked hyperplasia with increased thickening of the epidermis and immune cell infiltration

• psoriatic skin contains an infiltrate of CD45+ leukocytes composed of increased numbers of neutrophils, myeloid antigen-presenting cells and gamma-delta and alpha-beta T cells

• mice treated with a neutralizing antibody specific for IL-23p19 subunit show a decreased psoriatic phenotype on the ears and tail after 2 weeks of treatment

immune system

skin inflammation ( J:262723 )

• psoriatic skin contains an infiltrate of CD45+ leukocytes composed of increased numbers of neutrophils, myeloid antigen-presenting cells and gamma-delta and alpha-beta T cells

cellular

increased keratinocyte proliferation ( J:262723 )

• hyperproliferation of keratinocytes in the basal layer

cardiovascular system

abnormal blood vessel morphology ( J:262723 )

• increase in the number of enlarged CD31+ blood vessels is seen in the dermis, indicating an increase in dermal vasculature in psoriatic areas

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis 2		DOID:0080475	OMIM:602723	J:262723

Genotype
MGI:7861316

ht2

• Allelic Composition: Card14^em1Maak/Card14⁺
• Genetic Background: C57BL/6JJmsSlc-Card14^em1Maak

integument

abnormal hair follicle morphology ( J:360877 )

• P5 hair pores exhibit keratinous plugs

dilated hair follicle ( J:360877 )

• P5 skin shows dilatation of hair pores and keratinous plugs

hyperkeratosis ( J:360877 )

• P5 mice show mild hyperkeratosis

• at 2-3 weeks of age, mice show hyperkeratosis on the tail

• however, adults exhibit normal ear thickness

acanthosis ( J:360877 )

• P5 mice show mild acanthosis

scaly skin ( J:360877 )

• at 2-3 weeks of age, mice show scales on the tail, with the scales gradually increasing until 4-5 weeks of age, however the scales spontaneously disappear 6-7 weeks after birth

Genotype
MGI:6441186

ht3

• Allelic Composition: Card14^em2Tshu/Card14⁺
• Genetic Background: involves: C57BL/6

mortality/aging

postnatal lethality, incomplete penetrance ( J:289239 )

• normal numbers of pups are seen at 1-3 days after birth but fewer than the expected numbers of mice are seen at 3 weeks of age, indicating lower survival rate

integument

skin inflammation ( J:289239 )

• mice exhibit redness and swelling of ears and roughness of tails

• however, the systemic immune response is not affected, with mice showing normal spleen, thymus, and lymph node sizes and normal percentages of B220+ B cells, CD4+ and CD8+ T cells, CD11b+Ly6G+ neutrophils, CD11b+F4/80+ macrophages, and CD11c+MHCII+ dendritic cells

acanthosis ( J:289239 )

• epidermal acanthosis is seen in the ear, tail and back at 20 days of age

thick epidermis ( J:289239 )

• epidermal thickening of the ears and tail

• mice treated with an IL-17A-blocking antibody show partial reduction of ear thickness

psoriasis ( J:289239 )

• mice develop a psoriasis-like phenotype

• mice treated with the NF-KappaB inhibitor BAY11-7082 show attenuation of the skin phenotype

immune system

increased alpha-beta T cell number ( J:289239 )

• the percentage and total number of infiltrated IL-17A+ T cells is increased in the ears; most IL-17A+ lymphocytes in the ears are alpha-beta T cells

abnormal gamma-delta T cell number ( J:289239 )

• mice show increased proportion and absolute numbers of gamma-delta TCR^neg T cells but decreased percentage of gamma-delta TCR^mid and gamma-delta TCR^hi T cells

skin inflammation ( J:289239 )

• mice exhibit redness and swelling of ears and roughness of tails

• however, the systemic immune response is not affected, with mice showing normal spleen, thymus, and lymph node sizes and normal percentages of B220+ B cells, CD4+ and CD8+ T cells, CD11b+Ly6G+ neutrophils, CD11b+F4/80+ macrophages, and CD11c+MHCII+ dendritic cells

hematopoietic system

increased alpha-beta T cell number ( J:289239 )

• the percentage and total number of infiltrated IL-17A+ T cells is increased in the ears; most IL-17A+ lymphocytes in the ears are alpha-beta T cells

abnormal gamma-delta T cell number ( J:289239 )

• mice show increased proportion and absolute numbers of gamma-delta TCR^neg T cells but decreased percentage of gamma-delta TCR^mid and gamma-delta TCR^hi T cells

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis 2		DOID:0080475	OMIM:602723	J:289239

Genotype
MGI:6441187

ht4

• Allelic Composition: Card14^em3Tshu/Card14⁺
• Genetic Background: involves: C57BL/6

integument

skin inflammation ( J:289239 )

• redness and swelling of ears up to approximately 12 weeks of age

thick epidermis ( J:289239 )

• epidermal thickening in the tails at 18 and 36 days of age

psoriasis ( J:289239 )

• mice develop a weak psoriasis-like phenotype

immune system

skin inflammation ( J:289239 )

• redness and swelling of ears up to approximately 12 weeks of age

Genotype
MGI:6441189

cx5

• Allelic Composition: Card14^em2Tshu/Card14⁺; Il17a^tm1Cdon/Il17a^tm1Cdon
• Genetic Background: involves: C57BL/6

mortality/aging

prenatal lethality, complete penetrance ( J:289239 )

• no mice are obtained

Genotype
MGI:6441426

cx6

• Allelic Composition: Card14^em3Tshu/Card14⁺; Il17a^tm1Cdon/Il17a^tm1Cdon
• Genetic Background: involves: C57BL/6

immune system

decreased inflammatory response ( J:289239 )

• the increase in ear thickness indicative of skin inflammation that is seen in heterozygous Card14^em3Tshu mice is partially reduced