Phenotypes associated with this allele

• Allele Symbol: Apex1⁺
• Allele Name: wild type
• Allele ID: MGI:2439054
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Apex1^tm1Ecf/Apex1^+	either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)	MGI:2652224
ht2	Apex1^tm1Cur/Apex1^+	involves: 129S1/Sv	MGI:4358411
ht3	Apex1^tm1Djc/Apex1^+	involves: 129X1/SvJ * NTac:NIHS	MGI:3834458
cx4	Apex1^tm1Ecf/Apex1^+ Xpc^tm1Ecf/Xpc^tm1Ecf	involves: 129	MGI:2652241
cx5	Apex1^tm1Djc/Apex1^+ Tg(TacLIZa)A1Jsh/0	involves: 129X1/SvJ * C57BL/6 * NTac:NIHBS	MGI:3834457

Genotype
MGI:2652224

ht1

• Allelic Composition: Apex1^tm1Ecf/Apex1⁺
• Genetic Background: either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, incomplete penetrance ( J:70397 )

• fewer offspring than expected by Mendelian ratios, reduction was not statistically significant

• maternal antioxidant-enriched diet increased embryonic survival

cellular

abnormal cell death ( J:70397 )

• sensitivity to inducers of oxygen-mediated DNA damage

decreased cellular sensitivity to ultraviolet irradiation induced cell death ( J:70397 )

• resistant to cell death induced by UV radiation

increased cellular sensitivity to oxidative stress ( J:70397 )

• increased sensitivity to oxidative stress

• elevated levels of oxidative markers in serum

• antioxidant-enriched diet restores serum oxidative marker levels to those of wild-type

neoplasm

increased tumor incidence ( J:70397 )

• 25% developed microscopic tumors

increased lymphoma incidence ( J:70397 )

increased carcinoma incidence ( J:70397 )

increased sarcoma incidence ( J:70397 )

immune system

heart inflammation ( J:70397 )

myocarditis ( J:70397 )

pericarditis ( J:70397 )

cardiovascular system

cardiac fibrosis ( J:70397 )

• myocardial fibrosis

heart inflammation ( J:70397 )

myocarditis ( J:70397 )

pericarditis ( J:70397 )

homeostasis/metabolism

increased cellular sensitivity to oxidative stress ( J:70397 )

• increased sensitivity to oxidative stress

• elevated levels of oxidative markers in serum

• antioxidant-enriched diet restores serum oxidative marker levels to those of wild-type

Genotype
MGI:4358411

ht2

• Allelic Composition: Apex1^tm1Cur/Apex1⁺
• Genetic Background: involves: 129S1/Sv

cardiovascular system

increased mean systemic arterial blood pressure ( J:102294 )

• under anesthetized conditions

abnormal vascular smooth muscle physiology ( J:119957 )

• vascular smooth muscle cells exhibit increased apoptosis in response to Fas ligand compared with wild-type cells

decreased vasoconstriction ( J:102294 )

• in response to phenylephrine with L-NAME

increased vasoconstriction ( J:102294 )

• in response to phenylephrine

decreased vasodilation ( J:102294 )

• in response to acetycholine

abnormal vascular wound healing ( J:119957 )

• mice exhibit reduced neointimal hyperplasia in response to femoral artery wire injury compared to similarly treated wild-type mice

• mice fail to exhibit an increase in IL10 and IL1a levels following injury compared with similarly treated wild-type mice

homeostasis/metabolism

abnormal vascular wound healing ( J:119957 )

• mice exhibit reduced neointimal hyperplasia in response to femoral artery wire injury compared to similarly treated wild-type mice

• mice fail to exhibit an increase in IL10 and IL1a levels following injury compared with similarly treated wild-type mice

increased circulating interleukin-1 alpha level ( J:119957 )

• prior to injury

increased circulating interleukin-10 level ( J:119957 )

• prior to injury

abnormal nitric oxide homeostasis ( J:102294 )

• basal serum nitric oxide metabolites compared with wild-type mice

abnormal response/metabolism to endogenous compounds ( J:102294 )

• mice exhibits reduced vasodilation in response to acetylcholine and altered vascular tone in response to phenylephrine with or without L-NAME compared with wild-type mice

immune system

increased circulating interleukin-1 alpha level ( J:119957 )

• prior to injury

increased circulating interleukin-10 level ( J:119957 )

• prior to injury

muscle

abnormal vascular smooth muscle physiology ( J:119957 )

• vascular smooth muscle cells exhibit increased apoptosis in response to Fas ligand compared with wild-type cells

decreased vasoconstriction ( J:102294 )

• in response to phenylephrine with L-NAME

increased vasoconstriction ( J:102294 )

• in response to phenylephrine

decreased vasodilation ( J:102294 )

• in response to acetycholine

Genotype
MGI:3834458

ht3

• Allelic Composition: Apex1^tm1Djc/Apex1⁺
• Genetic Background: involves: 129X1/SvJ * NTac:NIHS

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:92957 )

♂ • in 9-month-old mice numbers of tubules containing apoptotic cells is higher than in controls (32.8% vs 19.3%); proportion of apoptotic spermatogonia is also higher (54.4% vs 17.3%) at 9 months

liver/biliary system

abnormal liver morphology ( J:92957 )

• at 9 months, centrilobular fatty changes surrounding the central vein are common (in 50% of animals) compared to wild-type controls

reproductive system

abnormal seminiferous tubule morphology ( J:92957 )

♂ • in 9-month-old mice numbers of tubules containing apoptotic cells is higher than in controls (32.8% vs 19.3%); proportion of apoptotic spermatogonia is also higher (54.4% vs 17.3%) at 9 months

Genotype
MGI:2652241

cx4

• Allelic Composition: Apex1^tm1Ecf/Apex1⁺; Xpc^tm1Ecf/Xpc^tm1Ecf
• Genetic Background: involves: 129

neoplasm

increased tumor incidence ( J:70397 )

• 16% developed microscopic tumors

cellular

oxidative stress ( J:70397 )

• increased sensitivity to oxidative stress

Genotype
MGI:3834457

cx5

• Allelic Composition: Apex1^tm1Djc/Apex1⁺; Tg(TacLIZa)A1Jsh/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * NTac:NIHBS

cellular

abnormal cell physiology ( J:92957 )

• spontaneous mutation frequency for mixed spermatogenic cells

abnormal DNA repair ( J:92957 )

• spontaneous mutation frequency for mixed spermatogenic cells from 9-month-old double mutant mice is 2-fold higher than for wild-type Apex1 hemizygous Tg mice

• spontaneous mutation frequency observed in liver samples in 3-month-old animals is significantly higher compared to age matched wild-type Apex1 hemizygous Tg mice; a further 1.5-fold increase is observed in samples from 9-month-old animals

• spontaneous mutation frequency observed in liver samples in 9-month-old animals is significantly higher compared to 3-month-old mice of the same genotype, suggesting an age-related increase in frequency

• spontaneous mutation frequency observed in spleen samples from 3-month-old mice is 2-fold higher than in controls and an additional 2-fold increase is found in samples from 9-month-old mice

homeostasis/metabolism

abnormal DNA repair ( J:92957 )

• spontaneous mutation frequency for mixed spermatogenic cells from 9-month-old double mutant mice is 2-fold higher than for wild-type Apex1 hemizygous Tg mice

• spontaneous mutation frequency observed in liver samples in 3-month-old animals is significantly higher compared to age matched wild-type Apex1 hemizygous Tg mice; a further 1.5-fold increase is observed in samples from 9-month-old animals

• spontaneous mutation frequency observed in liver samples in 9-month-old animals is significantly higher compared to 3-month-old mice of the same genotype, suggesting an age-related increase in frequency

• spontaneous mutation frequency observed in spleen samples from 3-month-old mice is 2-fold higher than in controls and an additional 2-fold increase is found in samples from 9-month-old mice