All Phenotypes Tsix<+> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	\Tsix^tm3Jtl/\Tsix⁺	involves: 129	MGI:3699214
ht2	\Tsix^tm2Jtl/\Tsix⁺	involves: 129 * M. m. castaneus	MGI:3699222
ht3	\Tsix/Xist^tm3Sado/\Tsix⁺	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:3772877
ht4	\Tsix^tm1Enl/\Tsix⁺	involves: 129S4/SvJae	MGI:2680289
cx5	\Tsix^tm1.1Awu/\Tsix⁺ \Tg(CAG-EGFP)50Osb/0 \Gt(ROSA)26Sor^tm1(rtTA)Awu/\Gt(ROSA)26Sor^tm1(rtTA)Awu	involves: 129P2/OlaHsd * 129S4/SvJae * C3H/HeSlc * C57BL/6J * C57BL/6Slc	MGI:5285848
cx6	\Tsix^tm1Enl/\Tsix⁺ \Xist^tm1Jae/\Xist⁺	involves: 129S4/SvJae	MGI:2680291
cx7	\Tsix^tm1Enl/\Tsix⁺ \Xist^tm1Sado/\Xist⁺	involves: 129S4/SvJae	MGI:3603005

Allelic Composition	\Tsix^tm3Jtl/\Tsix⁺
Genetic Background	involves: 129

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsix^tm3Jtl mutation (0 available); any Tsix mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased survivor rate ( J:117335 )

• only 10-20% of mutants survive to adulthood, when the allele is transmitted maternally

embryonic lethality at implantation, incomplete penetrance ( J:117335 )

• mutants die during the implantation stage when allele is transmitted maternally; ~20% of mutant blastocysts bearing maternal allele fail to attach in culture and ~60% show sparse trophoblast outgrowth and degenerate after several days in culture, whereas wild-type blastocysts show robust growth for up to 7 days

• ex vivo, no obvious defects are observed during preimplantation stage

• paternal transmission has no effect on viability

reproductive system

decreased litter size ( J:117335 )

• female mutants produce smaller litters

cellular

abnormal imprinting ( J:117335 )

• live births carrying mutant allele are decreased (males: 40 wild-type, 11 mutant and females: 66 wild-type, 6 mutant whereas ratio of mutants to wild-type for both sexes expected is 50:50); 75-90% of mutants bearing maternal allele die

Allelic Composition	\Tsix^tm2Jtl/\Tsix⁺
Genetic Background	involves: 129 * M. m. castaneus

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsix^tm2Jtl mutation (0 available); any Tsix mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

prenatal lethality ( J:57967 )

• in culture, female embryoid bodies with the mutant allele undergo massive cell death from days 8-15; female-specific lethality is observed in culture

cellular

abnormal dosage compensation, by inactivation of X chromosome ( J:57967 )

• mutants show defects random X chromosome inactivation

abnormal imprinting ( J:57967 )

• mutants show defects in imprinted and random X chromosome inactivation

Allelic Composition	\Tsix/Xist^tm3Sado/\Tsix⁺
Genetic Background	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsix/Xist^tm3Sado mutation (1 available); any Tsix mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

preweaning lethality, incomplete penetrance ( J:130425 )

• when this allele is inherited paternally no XX female offsprings are observed 3 weeks after birth and only 1 XO female is produced although sex ratios are normal at E7.5

embryo

abnormal embryo development ( J:130425 )

• authors state that when this allele is inherited maternally, mice exhibit phenotypes associated with a failure of imprinted X-inactivation as in Xist^tm1Jae Tsix^tm1Enl heterozygotes

cellular

abnormal DNA methylation ( J:130425 )

• methylation at the Xist promoter is lost and histone modifications at the promoter are aberrant

Allelic Composition	\Tsix^tm1Enl/\Tsix⁺
Genetic Background	involves: 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsix^tm1Enl mutation (1 available); any Tsix mutation (57 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal digestive system physiology ( J:68136 )

• 3-4X increase of apoptotic cells in colons after injury but not when there is no injury

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

embryonic growth retardation ( J:174672 )

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E0.5 exhibit severe developmental defect at E7.5 compared with control mice

• however, no developmental defect is observed when the allele is maternally inherited or when doxycycline treated blastocysts with paternally inherited Tsix^tm1.1Awu are transplanted into wild-type recipients

abnormal placenta labyrinth morphology ( J:174672 )

• when Tsix^tm1.1Awu is inherited maternally, female mice treated with doxycycline from E6.5 exhibit reduced density of fetal capillaries in the placental labyrinth compared with control mice

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit a severe defect in fetal capillaries compared with control mice

absent placental labyrinth ( J:174672 )

• in severely affected female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

small placenta ( J:174672 )

• at E13.5 in female mice treated with doxycycline from E6.5 or E7.5 when Tsix^tm1.1Awu is inherited paternally

• mild at E13.5 in female mice treated with doxycycline from E8.5 when Tsix^tm1.1Awu is inherited paternally

• however, female mice treated with doxycycline from E9.5 exhibit normal placenta size at E13.5 when the allele is inherited paternally

abnormal trophoblast layer morphology ( J:174672 )

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit a severe defect in all trophoblast cell types compared with control mice

abnormal trophoblast giant cell morphology ( J:174672 )

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit overabundant and massively enlarged compared to in control mice

increased trophoblast giant cell number ( J:174672 )

• in severely affected female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

absent spongiotrophoblast layer ( J:174672 )

• absent in severely affected female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

cellular

abnormal DNA methylation ( J:174672 )

• increased in female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

abnormal dosage compensation, by inactivation of X chromosome ( J:174672 )

• in female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

abnormal imprinting ( J:174672 )

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E0.5 fail to imprint and inactivated the paternal X chromosome unlike in control mice

cardiovascular system

abnormal capillary morphology ( J:174672 )

• when Tsix^tm1.1Awu is inherited maternally, female mice treated with doxycycline from E6.5 exhibit reduced density of fetal capillaries in the placental labyrinth compared with control mice

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit a severe defect in fetal capillaries compared with control mice

decreased capillary density ( J:174672 )

growth/size/body

embryonic growth retardation ( J:174672 )

• when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E0.5 exhibit severe developmental defect at E7.5 compared with control mice

Allelic Composition	\Tsix^tm1Enl/\Tsix⁺ \Xist^tm1Jae/\Xist⁺
Genetic Background	involves: 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsix^tm1Enl mutation (1 available); any Tsix mutation (57 available)
Xist^tm1Jae mutation (0 available); any Xist mutation (8 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

embryo

decreased embryo size ( J:68136 )

• 7 of 13 embryos were significantly smaller than normal at E8.5 and E9.5

• gastrulation was apparently normal however

• remaining embryos were grossly normal in size and morphology

growth/size/body

decreased embryo size ( J:68136 )

• 7 of 13 embryos were significantly smaller than normal at E8.5 and E9.5

• gastrulation was apparently normal however

• remaining embryos were grossly normal in size and morphology

Allelic Composition	\Tsix^tm1Enl/\Tsix⁺ \Xist^tm1Sado/\Xist⁺
Genetic Background	involves: 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsix^tm1Enl mutation (1 available); any Tsix mutation (57 available)
Xist^tm1Sado mutation (1 available); any Xist mutation (8 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:99399 )

• females that have both mutations on the paternal X chromosome die soon after implantation; however, females that have both mutations on the maternal X chromosome are viable and fertile

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