Phenotypes associated with this allele

• Allele Symbol: F3⁺
• Allele Name: wild type
• Allele ID: MGI:2436822
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	F3^tm1Jld/F3^+	involves: 129P2/OlaHsd * NIH Black Swiss	MGI:3846688
ht2	F3^tm1Dwr/F3^+	involves: 129S1/Sv * 129X1/SvJ	MGI:3846608
ht3	F3^tm1Gjb/F3^+	involves: 129X1/SvJ * C57BL/6	MGI:3846705

Genotype
MGI:3846688

ht1

• Allelic Composition: F3^tm1Jld/F3⁺
• Genetic Background: involves: 129P2/OlaHsd * NIH Black Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal blood coagulation ( J:33831 )

• the average clotting time activity in microsome preparations from the brain and lungs is half that observed in wild-type preparations

Genotype
MGI:3846608

ht2

• Allelic Composition: F3^tm1Dwr/F3⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

premature death ( J:99260 )

• 54% of mice live less than 36 weeks

pregnancy-related premature death ( J:99260 )

• more than 50% of female mice develop fatal thrombotic complications within 10 days of delivery

postnatal lethality ( J:99260 )

• fewer than expected mice survive to weaning

homeostasis/metabolism

increased circulating factor IX level ( J:99260 )

• plasma levels of coagulation factor IX are increased compared to in wild-type mice

increased circulating factor XI level ( J:99260 )

• plasma levels of coagulation factor XI are increased compared to in wild-type mice

increased circulating factor VIII level ( J:99260 )

• plasma levels of coagulation factor VIII are increased compared to in wild-type mice

abnormal blood coagulation ( J:99260 )

• TAT (thrombin-antithrombin) levels are increased compared to in wild-type mice

• plasma levels of coagulation factors VIII, IX, and XI are increased compared to in wild-type mice

• either before particle depletion without phospholipids or after particle-depletion with phospholipids, conditioned media from embryonic fibroblast cultures exhibits increased coagulant activity compared to media from wild-type cells

• mice exhibit widespread intravascular fibrin deposition unlike wild-type mice

decreased partial thromboplastin time ( J:99260 )

• activated partial thromboplastin time is shortened compared to in wild-type mice

abnormal thrombosis ( J:99260 )

• more than 50% of female mice develop fatal thrombotic complications within 10 days of delivery

• mice that die prematurely exhibit dilation and obstruction by a large white mass in the left superior vena cava, left jugular vein, inferior vena cava, coronary vein, right heart, aorta/aortic arch, pulmonary atrioles, and/or pulmonary artery often accompanied by calcium deposits and leukocyte infiltrates unlike in wild-type mice

• at E17.5, mice occasionally exhibit minimal fibrinogen deposition in the heart and/or liver unlike in wild-type mice

Genotype
MGI:3846705

ht3

• Allelic Composition: F3^tm1Gjb/F3⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6

immune system

immune system phenotype ( J:128891 )

N • despite reduced response to endotoxin in vitro, mice exhibit a normal response to endotoxin exposure due to increased F3 production

abnormal macrophage physiology ( J:128891 )

• endotoxin-stimulated macrophages exhibit 50% less tissue factor activity compared to in wild-type mice

abnormal chemokine secretion ( J:128891 )

• endotoxin-induced whole blood or macrophages produced less CXCL1 than similarly treated wild-type blood or macrophages regardless of inhibition by TNF-alpha

decreased interleukin-6 secretion ( J:128891 )

• endotoxin-induced whole blood or macrophages produced less IL6 than similarly treated wild-type blood or macrophages regardless of inhibition by TNF-alpha

hematopoietic system

abnormal macrophage physiology ( J:128891 )

• endotoxin-stimulated macrophages exhibit 50% less tissue factor activity compared to in wild-type mice