Phenotypes associated with this allele

• Allele Symbol: Eno2⁺
• Allele Name: wild type
• Allele ID: MGI:2430558
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Eno2^tm1(DTA)Hsak/Eno2^+ Gng7^tm2(cre/PGR)Mmsh/Gng7^+	involves: C57BL/6	MGI:3833957
cn2	Eno2^tm1(DTA)Hsak/Eno2^+ Tg(Gpr151-cre)#Ito/0	involves: C57BL/6	MGI:5588027

Genotype
MGI:3833957

cn1

• Allelic Composition: Eno2^tm1(DTA)Hsak/Eno2⁺; Gng7^{tm2(cre/PGR)Mmsh}/Gng7⁺
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Ablation of medium-spiny projection neurons in the striatum of Gng7^{tm2(cre/PGR)Mmsh}/Gng7⁺ Eno2^tm1(DTA)Hsak/Eno2⁺ mice

nervous system

increased neuron apoptosis ( J:145152 )

• strong TUNEL staining is seen in the caudate putamen, nucleus accumbens and olfactory tubercle 10 days after RU-486 injection

• 13 days after RU-486 injection the density of NeuN positive neurons is dramatically reduced in the caudate putamen and the number of NeuN positive neurons is reduced in the nucleus accumbens core and shell

abnormal medium spiny neuron morphology ( J:145152 )

• lack calbindin positive neurons in the striatum after RU-486 treatment

behavior/neurological

behavior/neurological phenotype ( J:145152 )

N • despite ablation of striatal neurons, no defects in motor coordination are detected

N • all behavioral phenotype data are from mice at 13 to 22 days after RU-486 treatment

abnormal learning/memory/conditioning ( J:145152 )

• treatment with RU-486 after long term memory formation and then testing for memory retention 14 days later shows an impairment in fear memory retention

abnormal cued conditioning behavior ( J:145152 )

• impaired conditioning when using a low intensity unconditioned stimulus but not when using a higher intensity unconditioned stimulus

• impaired development of long term but not of short term fear memory when using a low intensity unconditioned stimulus

impaired cued conditioning behavior ( J:210178 )

• 14 days after RU-486 treatment

• reduced freezing response on 2nd day in conditioning chamber

• unconditioned stimulus is a foot shock

• if RU-486 treatment occurs more than 24 hours after the unconditioned stimulus, fear memory is unaffected

abnormal motor learning ( J:145152 )

• fail to show any improvement in performance over repeated trial on an accelerating rotarod

hyperactivity ( J:145152 )

• increase in activity in an open field assay

cellular

increased neuron apoptosis ( J:145152 )

• strong TUNEL staining is seen in the caudate putamen, nucleus accumbens and olfactory tubercle 10 days after RU-486 injection

• 13 days after RU-486 injection the density of NeuN positive neurons is dramatically reduced in the caudate putamen and the number of NeuN positive neurons is reduced in the nucleus accumbens core and shell

Genotype
MGI:5588027

cn2

• Allelic Composition: Eno2^tm1(DTA)Hsak/Eno2⁺; Tg(Gpr151-cre)#Ito/0
• Genetic Background: involves: C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:214076 )

N • mice exhibit normal performance in fear conditioning tests and saccharin preference

impaired behavioral response to nicotine ( J:214076 )

• following systemic nicotine administration, mice fail to exhibit slowed down nose-poke reaction and enhanced adaptation unlike control mice

abnormal habituation to a new environment ( J:214076 )

• absence of habituation upon repeated exposure to a new environment

• however, exploration of a new environment is normal

impaired spatial learning ( J:214076 )

• in a corner avoidance test

abnormal spatial working memory ( J:214076 )

• poor spatial memory in a Morris water maze

• tendency to working memory deficits in a radial arm maze

increased anxiety-related response ( J:214076 )

• modest in an open field test (reduced time in center area) and an elevated plus maze (reduced time in open arms)

abnormal impulsive behavior control ( J:214076 )

• premature response in a 5-choice serial reaction time task (5-CSRTT)

• in a T maze, mice exhibit devalued reward by delay and effort compared with control mice

• in a saccharin preference test, mice exhibit poorly controlled response to reward compared with control mice

• however, erroneous nose-pokes are normal indicating unaltered spatial attention

hyperactivity ( J:214076 )

• tendency during the early night period in the home cage

• visit hyperactivity in free, nose-poke and drinking adaptation tests

• however, exploration of a new environment is normal

nervous system

abnormal midbrain morphology ( J:214076 )

• reduced acetylcholine concentration in interpeduncular nucleus

• reduced interpeduncular nucleus volume not due to cell loss

abnormal habenula morphology ( J:214076 )

• mice exhibit reduced number of neurons, specifically substance P-nergic and cholinergic cells, in the habenular complex medial and lateral nuclei compared with control mice

• reduced transverse area of fasciculus retroflux

abnormal cholinergic neuron morphology ( J:214076 )

• mice exhibit reduced number of neurons, specifically substance P-nergic and cholinergic cells, in the habenular complex medial and lateral nuclei compared with control mice

• reduced acetylcholine concentration in interpeduncular nucleus

decreased neuron number ( J:214076 )

• mice exhibit reduced number of neurons, specifically substance P-nergic and cholinergic cells, in the habenular complex medial and lateral nuclei compared with control mice

• however, the number of neurons in the paraventricular and reuniens thalamic nuclei is normal

decreased prepulse inhibition ( J:214076 )