Phenotypes associated with this allele

• Allele Symbol: Pou3f2^tm1Tno
• Allele Name: targeted mutation 1, Tetsuo Noda
• Allele ID: MGI:2429695
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pou3f2^tm1Tno/Pou3f2^tm1Tno	involves: 129S4/SvJae * C57BL/6J	MGI:3795356
cx2	Pou3f2^tm1Tno/Pou3f2^tm1Tno Pou3f3^tm1Tno/Pou3f3^tm1Tno	involves: 129S4/SvJae * C57BL/6J	MGI:3795357

Genotype
MGI:3795356

hm1

• Allelic Composition: Pou3f2^tm1Tno/Pou3f2^tm1Tno
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:30429 )

• homozygotes are born live and do not display obvious abnormalities but begin to die within several hours; more than half die within 2 days and all die within 10 days of birth

growth/size/body

postnatal growth retardation ( J:30429 )

• mice surviving >2 days display markedly retarded growth; body weight is half that of normal littermates at 4 days of age

endocrine/exocrine glands

abnormal neurohypophysis morphology ( J:30429 )

• posterior lobe is severely hypoplastic at 4 days of age

• no vasopressin-expressing nerve fibers are detected in posterior lobe

nervous system

abnormal neurohypophysis morphology ( J:30429 )

• posterior lobe is severely hypoplastic at 4 days of age

• no vasopressin-expressing nerve fibers are detected in posterior lobe

abnormal hypothalamus morphology ( J:30429 )

• the paraventricular nucleus and supraoptic nucleus are missing in mutant hypothalamus

• median eminence is extremely hypoplastic; anuclear light region of median eminence is specifically impaired

• all neurons forming the paraventricular nucleus (PVN) and supraoptic (SO) nucleus, including magnocellular neurons, are absent

• parvocellular neurons (that synthesize and secrete corticotropin-releasing factor, CRF and thyrotropin-releasing hormone, TRH) are absent from PVN

• somatostatinergic neurons in periventricular (PEV) region are absent

abnormal neuronal precursor cell number ( J:30429 )

• vasopressin-positive neuronal precursors are absent in hypothalamus at E14.5; neuronal precursors for PVN and SO die around E12.5 during migration from ventricular region

adipose tissue

absent subcutaneous adipose tissue ( J:30429 )

• subcutaneous adipose tissues are totally absent

abnormal brown adipose tissue morphology ( J:30429 )

• depots in mouse back do not contain any fatty deposition

liver/biliary system

hepatic steatosis ( J:30429 )

• fatty liver persists in mice surviving 1 week

integument

absent subcutaneous adipose tissue ( J:30429 )

• subcutaneous adipose tissues are totally absent

Genotype
MGI:3795357

cx2

• Allelic Composition: Pou3f2^tm1Tno/Pou3f2^tm1Tno; Pou3f3^tm1Tno/Pou3f3^tm1Tno
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

mortality/aging

neonatal lethality, complete penetrance ( J:77999 )

• expected number of double homozygotes are born, but all die within 1 hour of birth

nervous system

abnormal neuronal migration ( J:77999 )

• E14.5-born neurons occupy significantly altered positions relative to wild-type neurons; in wild-type these neurons are in layers V and VI, but in mutant cortex, the neurons remain in the intermediate zone

abnormal cortical ventricular zone morphology ( J:77999 )

• less cell proliferation is observed at E14.5 onward compared to wild-type

abnormal cerebellum development ( J:77999 )

abnormal cerebellar foliation ( J:77999 )

• less foliation observed at P0, with loosely packed Purkinje cells observed

enlarged lateral ventricles ( J:77999 )

abnormal cerebral cortex morphology ( J:77999 )

abnormal neocortex morphology ( J:77999 )

• severely affected, with marked reduction in thickness at P0

• cortical neurons have altered positioning in neocortex

abnormal stratification in cerebral cortex ( J:77999 )

• stratification of cortical neurons appears disorganized at P0

loss of cortex neurons ( J:77999 )

• upper-layer neurons are lost in mutants in late development

abnormal olfactory bulb morphology ( J:77999 )

• bulb exhibits hypoplasia

abnormal embryonic/fetal subventricular zone morphology ( J:77999 )

• reduction in proliferating cells is most severe in cortical subventricular zone (only 16.5% of wild-type cell proliferation)

cellular

abnormal neuronal migration ( J:77999 )

• E14.5-born neurons occupy significantly altered positions relative to wild-type neurons; in wild-type these neurons are in layers V and VI, but in mutant cortex, the neurons remain in the intermediate zone