Phenotypes associated with this allele

• Allele Symbol: Tln1^tm1Crit
• Allele Name: targeted mutation 1, David R Critchley
• Allele ID: MGI:2429594
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tln1^tm1Crit/Tln1^tm1Crit	involves: 129P2/OlaHsd * MF1	MGI:3774744

Genotype
MGI:3774744

hm1

• Allelic Composition: Tln1^tm1Crit/Tln1^tm1Crit
• Genetic Background: involves: 129P2/OlaHsd * MF1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:65873 )

• mice die between E8.5 and E9.5

embryo

abnormal embryo development ( J:65873 )

• at E7.5, embryos possess fewer cells than wild-type embryos

• however, apoptosis and cell cycle progression are normal at E7.5

abnormal gastrulation movements ( J:65873 )

• abnormal embryos display failure of cell migration at gastrulation

• inability of the extraembryonic mesoderm to migrate sufficiently results in failure of the exocoelomic cavity to form

abnormal ectoderm development ( J:65873 )

• at E6.5, the ectoderm is not thinned to a uniform columnar epithelial layer

abnormal mesoderm development ( J:65873 )

• at E7.5, the embryonic mesoderm accumulates as a mass on the posterior side of abnormal embryos, causing adjacent ectodermal cells to protrude into the amniotic cavity

embryonic growth arrest ( J:65873 )

• at E7.5

decreased embryo size ( J:65873 )

• at E6.5 and E7.5

abnormal embryonic tissue morphology ( J:65873 )

• at E6.5, the embryonic region is reduced compared to in wild-type mice

embryonic-extraembryonic boundary constriction ( J:65873 )

• a constriction between the embryonic and extraembryonic regions is commonly observed

abnormal extraembryonic tissue morphology ( J:65873 )

• at E7.5, the extraembryonic tissue is reduced in size compared to in wild-type mice

abnormal amniotic cavity morphology ( J:65873 )

• at E7.5, the amniotic cavity fails to divide

small amniotic cavity ( J:65873 )

• at E7.5, the amniotic cavity is significantly reduced in size compared to in wild-type mice

absent ectoplacental cavity ( J:65873 )

• at E7.5, the ectoplacental cavity has not formed

absent extraembryonic coelom ( J:65873 )

• at E7.5, the exocoelomic cavity has not formed

abnormal trophoblast layer morphology ( J:65873 )

• trophoblast cells cultured on fibronectin and laminin exhibit reduced spreading compared to wild-type cells

• after 5 days in culture, trophoblasts are either less well spread or detaching from the culture dish

small proamniotic cavity ( J:65873 )

• at E6.5, the pro-amniotic cavity is reduced in size

disorganized extraembryonic tissue ( J:65873 )

• at E7.5, mutant embryos show disorganised extraembryonic tissues

abnormal extraembryonic mesoderm development ( J:65873 )

• at E7.5, a reduction of extraembryonic mesoderm is observed

growth/size/body

decreased embryo size ( J:65873 )

• at E6.5 and E7.5