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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Zap70tm2.1Mmal
targeted mutation 2.1, Marie Malissen
MGI:2388918
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Zap70tm2.1Mmal/Zap70tm2.1Mmal B6.129P2-Zap70tm2.1Mmal MGI:3790315
cx2
 		Zap70tm2.1Mmal/Zap70tm2.1Mmal
Tg(TcrLCMV)327Sdz/0 		involves: 129P2/OlaHsd * BALB/cJ * C57BL/6 * DBA/2 MGI:3790318
cx3
 		Zap70tm2.1Mmal/Zap70tm2.1Mmal
Tg(TcraH-Y,TcrbH-Y)71Vbo/0 		involves: 129P2/OlaHsd * BALB/cJ * C57BL/6J * DBA/2J MGI:3790317


Genotype
MGI:3790315
hm1
Allelic
Composition		 Zap70tm2.1Mmal/Zap70tm2.1Mmal
Genetic
Background		 B6.129P2-Zap70tm2.1Mmal
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zap70tm2.1Mmal mutation (0 available); any Zap70 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• mice show no abnormal immune organ cellularity or T lymphocyte representation




Genotype
MGI:3790318
cx2
Allelic
Composition		 Zap70tm2.1Mmal/Zap70tm2.1Mmal
Tg(TcrLCMV)327Sdz/0
Genetic
Background		 involves: 129P2/OlaHsd * BALB/cJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrLCMV)327Sdz mutation (11 available)
Zap70tm2.1Mmal mutation (0 available); any Zap70 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• generation of CD8+/V alpha2high thymocytes and lymph node T cells is normal
• double negative and double positive cells from thymi of female mutants and wild-type mice have similar levels of CD5 expression
abnormal T cell morphology ( J:71099 )
• single positive thymocytes have lower CD5 surface expression than thymocytes from wild-type Tg(TcrLCMV)327Sdz or Tg(TcraH-Y,TcrbH-Y)71Vbo mice, or Zap70tm1.1Mmal/ Tg(TcrLCMV)327Sdz or Zap70tm1.1Mmal/ Tg(TcraH-Y,TcrbH-Y)71Vbo double mutants

hematopoietic system
abnormal T cell morphology ( J:71099 )
• single positive thymocytes have lower CD5 surface expression than thymocytes from wild-type Tg(TcrLCMV)327Sdz or Tg(TcraH-Y,TcrbH-Y)71Vbo mice, or Zap70tm1.1Mmal/ Tg(TcrLCMV)327Sdz or Zap70tm1.1Mmal/ Tg(TcraH-Y,TcrbH-Y)71Vbo double mutants




Genotype
MGI:3790317
cx3
Allelic
Composition		 Zap70tm2.1Mmal/Zap70tm2.1Mmal
Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic
Background		 involves: 129P2/OlaHsd * BALB/cJ * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (4 available)
Zap70tm2.1Mmal mutation (0 available); any Zap70 mutation (53 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• double negative and double positive cells from thymi of mutants and wild-type mice have similar levels of CD5 expression
abnormal thymus cell ratio ( J:71099 )
• CD8+/T3.70high cell number in thymus displays a 2-3-fold reduction compared to wild-type or homozygous Zap70tm1.1Mmal hemizygous transgenic animals
• a novel T3.70high/double positive T cell population comprising 30% of thymocytes is observed in mutant thymi compared to mice expressing the Tg(TcraH-Y,TcrbH-Y)71Vbo transgene alone
abnormal T cell morphology ( J:71099 )
• single positive thymocytes have lower CD5 surface expression than thymocytes from wild-type Tg(TcrLCMV)327Sdz or Tg(TcraH-Y,TcrbH-Y)71Vbo mice or Zap70tm1.1Mmal/ Tg(TcrLCMV)327Sdz or Zap70tm1.1Mmal/ Tg(TcraH-Y,TcrbH-Y)71Vbo animals
abnormal lymph node cell ratio ( J:71099 )
• CD8+/T3.70high cell number in lymph nodes displays a 2-3-fold reduction compared to wild-type or homozygous Zap70tm1.1Mmal hemizygous transgenic animals

hematopoietic system
abnormal thymus cell ratio ( J:71099 )
• CD8+/T3.70high cell number in thymus displays a 2-3-fold reduction compared to wild-type or homozygous Zap70tm1.1Mmal hemizygous transgenic animals
• a novel T3.70high/double positive T cell population comprising 30% of thymocytes is observed in mutant thymi compared to mice expressing the Tg(TcraH-Y,TcrbH-Y)71Vbo transgene alone
abnormal T cell morphology ( J:71099 )
• single positive thymocytes have lower CD5 surface expression than thymocytes from wild-type Tg(TcrLCMV)327Sdz or Tg(TcraH-Y,TcrbH-Y)71Vbo mice or Zap70tm1.1Mmal/ Tg(TcrLCMV)327Sdz or Zap70tm1.1Mmal/ Tg(TcraH-Y,TcrbH-Y)71Vbo animals

endocrine/exocrine glands
abnormal thymus cell ratio ( J:71099 )
• CD8+/T3.70high cell number in thymus displays a 2-3-fold reduction compared to wild-type or homozygous Zap70tm1.1Mmal hemizygous transgenic animals
• a novel T3.70high/double positive T cell population comprising 30% of thymocytes is observed in mutant thymi compared to mice expressing the Tg(TcraH-Y,TcrbH-Y)71Vbo transgene alone




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
09/30/2025
MGI 6.24 		The Jackson Laboratory