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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Zap70tm1.1Mmal
targeted mutation 1.1, Marie Malissen
MGI:2388913
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Zap70tm1.1Mmal/Zap70tm1.1Mmal B6.129P2-Zap70tm1.1Mmal MGI:3790314
cx2
 		Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcrLCMV)327Sdz/0 		involves: 129P2/OlaHsd * BALB/cJ * C57BL/6 * DBA/2 MGI:3790319
cx3
 		Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcraH-Y,TcrbH-Y)71Vbo/0 		involves: 129P2/OlaHsd * BALB/cJ * C57BL/6J * DBA/2J MGI:3790316


Genotype
MGI:3790314
hm1
Allelic
Composition		 Zap70tm1.1Mmal/Zap70tm1.1Mmal
Genetic
Background		 B6.129P2-Zap70tm1.1Mmal
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zap70tm1.1Mmal mutation (0 available); any Zap70 mutation (55 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• mice show no abnormal immune organ cellularity or T lymphocyte representation




Genotype
MGI:3790319
cx2
Allelic
Composition		 Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcrLCMV)327Sdz/0
Genetic
Background		 involves: 129P2/OlaHsd * BALB/cJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcrLCMV)327Sdz mutation (8 available)
Zap70tm1.1Mmal mutation (0 available); any Zap70 mutation (55 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• generation of CD8+/V alpha2high thymocytes and lymph node T cells is normal
• double negative and double positive cells from thymi of female mutants and wild-type mice have similar levels of CD5 expression
abnormal T cell proliferation ( J:71099 )
• when challenged with subnanomolar concentrations of p33 peptide in vitro, T cells proliferate more than wild-type Tg(TcrLCMV)327Sdz T cells
abnormal cytokine secretion ( J:71099 )
• T cells produce 2-3-fold more IL-2 in response to graded p33 concentrations in vitro compared to stimulated wild-type Tg(TcrLCMV)327Sdz or Zap70tm2.1Mmal/ Tg(TcrLCMV)327Sdz T cells
• this is not observed at a single-cell level of cytokine production, indicating that increased IL-2 is result of greater T cell numbers

hematopoietic system
abnormal T cell proliferation ( J:71099 )
• when challenged with subnanomolar concentrations of p33 peptide in vitro, T cells proliferate more than wild-type Tg(TcrLCMV)327Sdz T cells

cellular
abnormal T cell proliferation ( J:71099 )
• when challenged with subnanomolar concentrations of p33 peptide in vitro, T cells proliferate more than wild-type Tg(TcrLCMV)327Sdz T cells




Genotype
MGI:3790316
cx3
Allelic
Composition		 Zap70tm1.1Mmal/Zap70tm1.1Mmal
Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic
Background		 involves: 129P2/OlaHsd * BALB/cJ * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (3 available)
Zap70tm1.1Mmal mutation (0 available); any Zap70 mutation (55 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:71099 )
N
• numbers of CD8+/T3.70high thymocytes and lymph node T cells are normal, indicating that the Zap70 mutation has no effect on positive selection of T cells
• double negative and double positive cells from thymi of female mutants and wild-type mice have similar levels of CD5 expression




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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory