Phenotypes associated with this allele

• Allele Symbol: Top3a^tm1Jcw
• Allele Name: targeted mutation 1, James C Wang
• Allele ID: MGI:2388159
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Top3a^tm1Jcw/Top3a^tm1Jcw	involves: 129S6/SvEvTac * C57BL/6	MGI:2655619

Genotype
MGI:2655619

hm1

• Allelic Composition: Top3a^tm1Jcw/Top3a^tm1Jcw
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:45652 )

• homozygotes die following implantation and the induction of decidualization

• histological sections of 7.5-dpc deciduae from heterozygous intercrosses revealed two distinct classes: those containing embryos of normal appearance that were readily identified as either heterozygous or wild-type, and those that were empty or contained insufficient embryonic material for genotyping

embryo

abnormal inner cell mass proliferation ( J:45652 )

• in vitro, 10 of 19 mutant preimplantation blastocysts cultured on mouse feeder layer cells displayed poor proliferation of inner cell mass during the first few days after hatching, and complete cessation of growth thereafter

• 7 of the remaining 9 failed to hatch or attach to the feeder layer and two exhibited poor trophoblast outgrowth and no identifiable inner cell mass

cellular

abnormal inner cell mass proliferation ( J:45652 )

• in vitro, 10 of 19 mutant preimplantation blastocysts cultured on mouse feeder layer cells displayed poor proliferation of inner cell mass during the first few days after hatching, and complete cessation of growth thereafter

• 7 of the remaining 9 failed to hatch or attach to the feeder layer and two exhibited poor trophoblast outgrowth and no identifiable inner cell mass