Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
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immune system
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• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type
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• B cell development is arrested at the transition between pro- to pre- B cells with cells accumulating at fraction B (CD43+ CD45+ HSA+ BP-1-) and terminally arresting at fraction C (CD43+ CD45+ HSAmed BP-1+)
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• mature B cells are not found in the periphery
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hematopoietic system
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• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type
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• B cell development is arrested at the transition between pro- to pre- B cells with cells accumulating at fraction B (CD43+ CD45+ HSA+ BP-1-) and terminally arresting at fraction C (CD43+ CD45+ HSAmed BP-1+)
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• mature B cells are not found in the periphery
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
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immune system
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• B cells are not found in the periphery
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• mucosal-associated invariant T cells (MAIT) that express the canonical mVa19-Ja33 TCR rearrangement and are typically found in the gut lamina propia are virtually absent in these mice
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• IgM is not detected in the sera
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hematopoietic system
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• B cells are not found in the periphery
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• mucosal-associated invariant T cells (MAIT) that express the canonical mVa19-Ja33 TCR rearrangement and are typically found in the gut lamina propia are virtually absent in these mice
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• IgM is not detected in the sera
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
Tg(IGK@yK1)9.2CJkbv mutation
(0 available)
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hematopoietic system
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• all B cells express human mu heavy chain immunoglobulin with most of the B cells also expressing human delta heavy chain
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• late pro-B cell numbers are increased more than 2-fold compared to wild-type mice suggesting a less efficient V to DJ joining process
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• the presence of the two transgenes rescues the block in B cell development that occurs in Igh-Jtm1Jkbv development
• mature B cells are found in the bone marrow, spleen and blood in numbers corresponding to 58%, 55%, and 30% of wild-type levels
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• antibodies containing human mu heavy chain are found in the serum at a concentration of 350 micrograms per ml, a level over 500 fold higher than found in transgenic mice without the Igh-J targeted mutation
• the serum concentration of antibodies with human mu heavy chain is only 2- to 3- fold lower than the concentration of IgM found in humans
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immune system
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• all B cells express human mu heavy chain immunoglobulin with most of the B cells also expressing human delta heavy chain
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• late pro-B cell numbers are increased more than 2-fold compared to wild-type mice suggesting a less efficient V to DJ joining process
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• the presence of the two transgenes rescues the block in B cell development that occurs in Igh-Jtm1Jkbv development
• mature B cells are found in the bone marrow, spleen and blood in numbers corresponding to 58%, 55%, and 30% of wild-type levels
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• antibodies containing human mu heavy chain are found in the serum at a concentration of 350 micrograms per ml, a level over 500 fold higher than found in transgenic mice without the Igh-J targeted mutation
• the serum concentration of antibodies with human mu heavy chain is only 2- to 3- fold lower than the concentration of IgM found in humans
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
Tg(IGK@yK1)9.2CJkbv mutation
(0 available)
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immune system
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• 43% of B cells express human mu heavy chain and human kappa light chain
• 57% of B cells express human mu heavy chain and mouse lamda light chain
• no co-expression of human and mouse light chain is found
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cells are found at a level 10% of that in wild-type mice
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• human antibodies occur in the sera of mice at 10 micrograms per ml
• this concentration is several hundred fold higher than occurs in transgenic mice that do not have endogenous Ig heavy and light chains inactivated
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hematopoietic system
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• 43% of B cells express human mu heavy chain and human kappa light chain
• 57% of B cells express human mu heavy chain and mouse lamda light chain
• no co-expression of human and mouse light chain is found
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cells are found at a level 10% of that in wild-type mice
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• human antibodies occur in the sera of mice at 10 micrograms per ml
• this concentration is several hundred fold higher than occurs in transgenic mice that do not have endogenous Ig heavy and light chains inactivated
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
|
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immune system
|
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• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type
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• mature B cells are absent from the spleen and blood
(J:70620)
• mature B cells are not found in the periphery
(J:137738)
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• B cells are not found in this mouse strain
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hematopoietic system
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• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type
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• mature B cells are absent from the spleen and blood
(J:70620)
• mature B cells are not found in the periphery
(J:137738)
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• B cells are not found in this mouse strain
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
|
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|
immune system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
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• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
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• pre-B cells numbers in the bone marrow are 30% of wild-type
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hematopoietic system
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
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• late pro B-cells accumulate at numbers 3- to 4- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
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• pre-B cells numbers in the bone marrow are 30% of wild-type
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
|
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immune system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
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• immature circulating B cells are at levels that are 80% of normal
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
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• the levels of circulating mature B cells are 20% of the numbers found in wild-type
• these B cells express human mu heavy chain
• these mature B cells numbers are twice those found in Ihg-J null mice that are hemizygote for the transgene
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• large pre-B cells numbers in the bone marrow are 50% of wild-type
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• antibodies containing human mu heavy chains are found in the sera at levels of 60 micrograms per ml
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hematopoietic system
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
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• immature circulating B cells are at levels that are 80% of normal
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
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• the levels of circulating mature B cells are 20% of the numbers found in wild-type
• these B cells express human mu heavy chain
• these mature B cells numbers are twice those found in Ihg-J null mice that are hemizygote for the transgene
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• large pre-B cells numbers in the bone marrow are 50% of wild-type
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• antibodies containing human mu heavy chains are found in the sera at levels of 60 micrograms per ml
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
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immune system
|
N |
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu or gamma2 heavy chains are found in the sera at levels of 200 micrograms per ml
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hematopoietic system
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu or gamma2 heavy chains are found in the sera at levels of 200 micrograms per ml
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Tg(IGH@*)SALed mutation
(0 available)
|
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immune system
|
N |
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
|
|
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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hematopoietic system
|
|
• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
Tg(IGK@yK1)9.2CJkbv mutation
(0 available)
|
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immune system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
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• B cells newly emerging from the bone marrow are at levels that are 10% of that in wild-type mice
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
|
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• pre-B cells numbers in the bone marrow are 30% of wild-type
|
hematopoietic system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
|
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• B cells newly emerging from the bone marrow are at levels that are 10% of that in wild-type mice
|
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
|
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|
• pre-B cells numbers in the bone marrow are 30% of wild-type
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
Tg(IGK@yK1)9.2CJkbv mutation
(0 available)
|
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immune system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
|
|
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
|
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• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
|
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• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
|
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• pre-B cells numbers in the bone marrow are 70% of wild-type
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 150 and 120 micrograms per ml, respectively
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hematopoietic system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
|
|
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
|
|
|
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
|
|
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
|
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• pre-B cells numbers in the bone marrow are 70% of wild-type
|
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 150 and 120 micrograms per ml, respectively
|
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH1)2BJkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
|
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• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
|
|
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
|
|
|
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
|
|
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
|
|
|
• pre-B cells numbers in the bone marrow are 70% of wild-type
|
hematopoietic system
|
|
• B cells develop inefficiently with a partial block occurring at the transition from pre- to pro- B cells
|
|
|
• B cells newly emerging from the bone marrow are at levels that are 30% of that in wild-type mice
|
|
|
• late pro B-cells accumulate at numbers 3- fold higher than in wild-type at the fraction C (CD43+ CD45+ HSAmed BP-1+) step
|
|
|
• the levels of circulating mature B cells are 10% of the numbers found in wild-type
• these B cells express human mu heavy chain
|
|
|
• pre-B cells numbers in the bone marrow are 70% of wild-type
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
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N |
• the presence of the transgene prevents the arrest in B cell differentiation that occurs in Igh-Jtm1Jkbv homozygotes
|
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|
• the levels of immature B cells found in circulation are 70% that found in wild-type mice
|
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
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• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively
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hematopoietic system
|
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• the levels of immature B cells found in circulation are 70% that found in wild-type mice
|
|
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• the levels of mature B cells found in the spleen are 50% and 70% respectively, of those found in wild-type mice
• these B cells express human mu heavy chain
|
|
|
• antibodies containing human mu and kappa immunoglobulin chains are found in the sera at levels of 300 and 530 micrograms per ml, respectively
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2BM)7Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
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• mice have 60-80% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG1
• IgM levels in sera ranged from 110-140 micrograms/ml
• IgG1 levels in sera ranged from 172-298 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
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hematopoietic system
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• mice have 60-80% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG1
• IgM levels in sera ranged from 110-140 micrograms/ml
• IgG1 levels in sera ranged from 172-298 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2CM)2Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
|
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• mice have 80-100% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG2
• IgM levels in sera ranged from 27.3-109.4 micrograms/ml
• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
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hematopoietic system
|
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• mice have 80-100% the number of B cells as wild-type mice
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• unimmunized mice produce significant levels of human IgM and IgG2
• IgM levels in sera ranged from 27.3-109.4 micrograms/ml
• IgG2 levels in sera ranged from 37.6-665.3 micrograms/ml
• immunizing mice with human antigens leads to production of antigen-specific antibodies with human sequences
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yHG1/2)1Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
|
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• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences
|
hematopoietic system
|
|
• immunizing mice with human antigens leads to production of antigen-specific IgG1 antibodies with human sequences
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yHG4)1Amgn mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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|
immune system
|
|
• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences
|
hematopoietic system
|
|
• immunizing mice with human antigens leads to production of antigen-specific IgG4 antibodies with human sequences
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Jkbv mutation
(0 available);
any
Igh-J mutation
(13 available)
Igkctm1Jkbv mutation
(0 available);
any
Igkc mutation
(9 available)
Tg(IGH@yH2)J9.2Jkbv mutation
(0 available)
Tg(IGK@yK2)J23.1Jkbv mutation
(0 available)
|
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immune system
|
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• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
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• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
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• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
|
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
|
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• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen
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hematopoietic system
|
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• all B cells express human IgM and high levels of B220
• about 80% of B cells exclusively express human kappa light chain
• only 15% of B cells express endogenous mouse lambda mouse chain
|
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|
• productive rearrangement of the VDJ segments of the Tg(IGHyH2)J9.2Jkbv transgene occurs in B cells
• the V gene segments used are widely distributed in the variable region of the transgene with predominant usage similar to what is observed in humans
• J segment usage also resembles usage in human B cells
|
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• productive rearrangement of the VJ segments of the Tg(IGKyK2)J23.1Jkbv transgene occurs in B cells
• V and J gene segment usage resembles what is observed in human B cells
|
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• the presence of the two transgenes overcomes the block in B cell development that occurs in Igh-Jtm1Jkbv Igk-Ctm1Jkbv double homozygotes
• mature B cell numbers are 60% of that found in wild-type mice
|
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|
• antibodies containing human immunoglobulin chains are present in the sera
• concentrations of human mu and gamma2 heavy chains, and kappa light chains are 700, 600, and 800 micrograms per ml, respectively
• after immunization with antigen, IgG2 levels increase to 2.5 mg per ml
• high affinity human antibodies can be generated by immunizing mice with human antigen
|
Analysis Tools