Phenotypes associated with this allele

• Allele Symbol: Adcyap1^tm1Nms
• Allele Name: targeted mutation 1, Nancy M Sherwood
• Allele ID: MGI:2386973
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Adcyap1^tm1Nms/Adcyap1^tm1Nms	B6.129-Adcyap1^tm1Nms	MGI:3822735
hm2	Adcyap1^tm1Nms/Adcyap1^tm1Nms	either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: C57BL/6)	MGI:2447674

Genotype
MGI:3822735

hm1

• Allelic Composition: Adcyap1^tm1Nms/Adcyap1^tm1Nms
• Genetic Background: B6.129-Adcyap1^tm1Nms

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:131950 )

• litters born to female homozygotes are of normal size on the day of birth but show higher mortality than litters born to wild-type dams up to 21 days after birth

behavior/neurological

abnormal sexual interaction ( J:131950 )

♀ • 33% of female homozygotes have seminal plugs on more than one day in a 7-day pairing period, indicating abnormal mating patterns

♀ • however, female homozygotes display normal puberty onset, estrous cycles, and seminal plugs when paired with a male of proven fertility

reproductive system

impaired embryo implantation ( J:131950 )

♀ • only 13% of female homozygotes have implanted embryos 6.5 days after mating vs 81% of wild-type females

reduced female fertility ( J:131950 )

♀ • only 21% of female homozygotes give birth to litters vs 100% of wild-type females

♀ • in a pseudopregnancy test, only 40% of female homozygotes become pregnant and display a continuous diestrous, as expected; the remaining 60% continue cycling and fail to enter pseudopregnancy

♀ • however, ovulation, ovarian histology, fertilization and first cleavage of released eggs appear normal

growth/size/body

slow postnatal weight gain ( J:131950 )

• heterozygous litters born to female homozygotes show lower growth curves than litters born to wild-type dams

• litters initially display identical birth weights but those nursed by mutant dams show a significantly lower body weight at day 3, and this difference persists to day 9

• after day 9, litter weights stay equivalent between the two groups

homeostasis/metabolism

decreased circulating prolactin level ( J:131950 )

• on day 6.5 after mating, average prolactin levels of female homozygotes are reduced to ~30% of wild-type levels

decreased circulating progesterone level ( J:131950 )

• on day 6.5 after mating, average progesterone levels of female homozygotes are reduced to ~30% of wild-type levels

Genotype
MGI:2447674

hm2

• Allelic Composition: Adcyap1^tm1Nms/Adcyap1^tm1Nms
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: C57BL/6)

mortality/aging

postnatal lethality, complete penetrance ( J:71968 , J:79468 )

• most dead by second week postnatal (J:71968)

• survival increased when housed at 24C (76% survived to day 14 postnatal vs. 11% at 21C) (J:79468)

adipose tissue

decreased white adipose tissue amount ( J:71968 )

• subcutaneous white fat deposits totally depleted at death

growth/size/body

weight loss ( J:71968 )

• after day 4 postnatal, failed to gain weight, then lost weight

homeostasis/metabolism

impaired adaptive thermogenesis ( J:79468 )

• when removed from mothers, pups lost temperature faster than wild-type littermates

hypoglycemia ( J:71968 )

• in fasted state

increased circulating corticosterone level ( J:71968 )

increased circulating insulin level ( J:71968 )

• in fasted state

increased circulating ketone body level ( J:71968 )

increased circulating cholesterol level ( J:71968 )

increased circulating free fatty acids level ( J:71968 )

increased circulating triglyceride level ( J:71968 )

decreased liver glycogen level ( J:71968 )

liver/biliary system

decreased liver glycogen level ( J:71968 )

microvesicular hepatic steatosis ( J:71968 )

muscle

abnormal skeletal muscle morphology ( J:71968 )

• intracellular fat accumulation