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Allele Symbol Allele Name Allele ID |
Gopctm1.1Tno targeted mutation 1.1, Tetsuo Noda MGI:2386794 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• spermatozoa tail coiling is abnormal, the mitochondrial sheath is stratified and outer dense fibers are absent
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• spermatozoa tail coiling is abnormal, the mitochondrial sheath is stratified and outer dense fibers are absent
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Ultrastructural analysis of spermatogenic cells from wild type and Gopctm1.1Tno/Gopctm1.1Tno mice
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• at 20 weeks of age, sperm motility is significantly reduced (6.9% +/-4.4% compared to 59.3% +/-7.5% in control sperm)
• most motile sperm present display only sluggish movement
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• defects in spermatogenesis are noted as early as step 2-3 spermatids, when fragmentation of acrosomal caps is already observed
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• an abnormal arrangement of mitochondria in the mitochondrial sheath is observed
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• some mutant epididymidal spermatozoa have their tails coiled around their nuclei; also observed in mature testicular spermatids
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• early round spermatids exhibit fragmentation of the acrosomal cap and abnormal large vesicles caused by defective fusion of the Golgi-derived transport vesicles to the acrosome
• elongated spermatids and epididymal spermatozoa display prominent acrosome vacuolization, loss of adhesion to the nucleus, and total acrosome loss
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• all mutant epididymidal spermatozoa exhibit a round or ovoid head shape; also observed in mature testicular spermatids
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• in some cases, a complete loss of acrosomes is observed
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• loss of adhesion to the nucleus is often observed
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• all elongated spermatids and epididymal spermatozoa display malformed nuclei with a round or ovoid morphology
• occasional vacuolization or invagination give the nucleus a crescent-shaped structure
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• male homozygotes are sterile
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• intracytoplasmic sperm injection (ICSI) of abnormal mutant sperm into oocytes results in cleavage into blastocysts only when injected oocytes are stimulated electrically 30 min after ICSI
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• an abnormal arrangement of mitochondria in the mitochondrial sheath is observed
|
|
|
• some mutant epididymidal spermatozoa have their tails coiled around their nuclei; also observed in mature testicular spermatids
|
|
|
• early round spermatids exhibit fragmentation of the acrosomal cap and abnormal large vesicles caused by defective fusion of the Golgi-derived transport vesicles to the acrosome
• elongated spermatids and epididymal spermatozoa display prominent acrosome vacuolization, loss of adhesion to the nucleus, and total acrosome loss
|
|
|
• all mutant epididymidal spermatozoa exhibit a round or ovoid head shape; also observed in mature testicular spermatids
|
|
|
• in some cases, a complete loss of acrosomes is observed
|
|
|
• loss of adhesion to the nucleus is often observed
|
|
|
• all elongated spermatids and epididymal spermatozoa display malformed nuclei with a round or ovoid morphology
• occasional vacuolization or invagination give the nucleus a crescent-shaped structure
|
|
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• at 20 weeks of age, sperm motility is significantly reduced (6.9% +/-4.4% compared to 59.3% +/-7.5% in control sperm)
• most motile sperm present display only sluggish movement
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Mouse Models of Human Disease |
DO ID | OMIM ID(s) | Ref(s) | |
| azoospermia | DOID:14227 | J:78599 | ||
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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