Analysis Tools
immune system
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• homozygotes display normal development and cellular composition of lymphoid organs relative to wild-type controls
• upon challenge with the intestinal parasitic nematode N. brasiliensis, homozygotes display normal induction of TH2 responses, as shown by normal IL-4 and IL-5 production, normal serum IgE levels and normal numbers of eosinophils in peripheral blood
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• after immunization with soluble recombinant VSV glycoprotein (VSV-G), homozygotes are unable to switch efficiently to IgG after primary immunization despite a normal IgM response, suggesting that T-cell B-cell interaction is impaired
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• homozygotes show a slight decrease in the number of germinal centers in the spleen white pulp relative to wild-type controls
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• homozygotes show a slight decrease in the size of germinal centers in the spleen white pulp relative to wild-type controls
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• homozygotes show a slight decrease in the number and size of germinal centers in the lymph node cortex relative to wild-type controls
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• in response to type II T-cell-independent antigen TNP-Ficoll, homozygotes exhibit normal B cell priming (as shown by normal IgM titers and germinal center formation) but display higher IgG1 and IgG2a titers than wild-type controls
• in response to low doses (10 ug) of soluble T-cell-dependent antigen TNP-KLH without adjuvants, both IgM and IgG1 and IgG2a responses are significantly reduced relative to those in wild-type controls
• however, in response to 10 ug TNP-KLH in CFA adjuvant, homozygotes display higher secondary IgM titers and normal IgG responses relative to wild-type controls
• no differences in TNP-specific IgG1 levels are observed after primary immunization and after a booster with 20 ug of TNP-KLH in alum, indicating that poor T-cell-dependent antibody responses can be overcome by the administration of antigen plus adjuvant
• after infection with Vesicular Stomatitis Virus (VSV) and different recombinant VSV antigens, homozygotes mount a normal T-cell-independent IgM response but show reduced T-cell-dependent IgG antibody responses relative to wild-type controls
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• at 8 weeks of age, non-immunized homozygotes show a 1.8-fold reduction in serum IgG1 levels; also observed at 8 months of age
• in contrast, serum levels of IgM, IgG2a, IgG2b, IgG3, IgA, and IgE remain normal
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hematopoietic system
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• after immunization with soluble recombinant VSV glycoprotein (VSV-G), homozygotes are unable to switch efficiently to IgG after primary immunization despite a normal IgM response, suggesting that T-cell B-cell interaction is impaired
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• homozygotes show a slight decrease in the number of germinal centers in the spleen white pulp relative to wild-type controls
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• homozygotes show a slight decrease in the size of germinal centers in the spleen white pulp relative to wild-type controls
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• at 8 weeks of age, non-immunized homozygotes show a 1.8-fold reduction in serum IgG1 levels; also observed at 8 months of age
• in contrast, serum levels of IgM, IgG2a, IgG2b, IgG3, IgA, and IgE remain normal
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