Phenotypes associated with this allele

• Allele Symbol: Rorc^tm1Litt
• Allele Name: targeted mutation 1, Dan R Littman
• Allele ID: MGI:2384142
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rorc^tm1Litt/Rorc^tm1Litt	involves: 129P2/OlaHsd	MGI:3767257
cx2	Rorc^tm1Litt/Rorc^tm1Litt Tcra-J^tm3.1Krg/Tcra-J^tm3.1Krg	involves: 129P2/OlaHsd * 129S6/SvEvTac	MGI:3767256

Genotype
MGI:3767257

hm1

• Allelic Composition: Rorc^tm1Litt/Rorc^tm1Litt
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:158663 )

• highly susceptible to C. rodentium and all die at day 10-12 post infection

cellular

abnormal cell cycle ( J:248565 )

• an increased number of thymocytes enter DNA synthesis (S phase) as compared to wild-type

increased thymocyte apoptosis ( J:248565 )

• decreased thymic cellularity due to accelerated apoptosis

colonic necrosis ( J:158663 )

• multifocal necrosis in colon post infection with C. rodentium

endocrine/exocrine glands

increased thymocyte apoptosis ( J:248565 )

• decreased thymic cellularity due to accelerated apoptosis

decreased thymocyte number ( J:248565 )

hematopoietic system

increased thymocyte apoptosis ( J:248565 )

• decreased thymic cellularity due to accelerated apoptosis

abnormal T cell morphology ( J:248565 )

• thymocytes are enlarged

decreased thymocyte number ( J:248565 )

abnormal T cell differentiation ( J:128344 , J:248565 )

• primary rearrangement of 5' Jalpha segments is reduced to 54% and central Jalpha segments is reduced 18.2% (J:128344)

• cultured DN (CD4- CD8-) thymocytes do not differentiate into DP (CD4+ CD8+) thymocytes (J:248565)

• in vivo, DP thymocyte frequency and number is decreased, however, DN frequency and absolute number is similar to wild-type (J:248565)

abnormal CD8-positive, alpha-beta T cell differentiation ( J:248565 )

• CD8+ thymocytes remain immature CD24 TCR cells

abnormal T-helper 17 cell differentiation ( J:248565 )

• cultured CD4+ T cells are defective in TH17 differentiation under TH17 priming conditions

• T cells fail to differentiate in IL17-producing cells, however T cell can differentiate into TH1, TH2 and regulator T cells

decreased double-positive T cell number ( J:248565 )

decreased CD4-positive, alpha-beta T cell number ( J:248565 )

• frequency and number of CD4+ T cells is decreased as compared to wild-type

immune system

increased thymocyte apoptosis ( J:248565 )

• decreased thymic cellularity due to accelerated apoptosis

colitis ( J:158663 )

• inflammation, and edema post infection with C. rodentium

abnormal T cell morphology ( J:248565 )

• thymocytes are enlarged

decreased thymocyte number ( J:248565 )

abnormal T cell differentiation ( J:128344 , J:248565 )

• primary rearrangement of 5' Jalpha segments is reduced to 54% and central Jalpha segments is reduced 18.2% (J:128344)

• cultured DN (CD4- CD8-) thymocytes do not differentiate into DP (CD4+ CD8+) thymocytes (J:248565)

• in vivo, DP thymocyte frequency and number is decreased, however, DN frequency and absolute number is similar to wild-type (J:248565)

abnormal CD8-positive, alpha-beta T cell differentiation ( J:248565 )

• CD8+ thymocytes remain immature CD24 TCR cells

abnormal T-helper 17 cell differentiation ( J:248565 )

• cultured CD4+ T cells are defective in TH17 differentiation under TH17 priming conditions

• T cells fail to differentiate in IL17-producing cells, however T cell can differentiate into TH1, TH2 and regulator T cells

decreased double-positive T cell number ( J:248565 )

decreased CD4-positive, alpha-beta T cell number ( J:248565 )

• frequency and number of CD4+ T cells is decreased as compared to wild-type

absent lymph nodes ( J:248565 )

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:248565 )

♀ • EAE development is attenuated in females immunized with EAE-inducing MOG epitope

increased susceptibility to bacterial infection ( J:158663 )

• display weight loss, colitis and severe disruption of the epithelial layer in the colon after infection with Citrobacter rodentium

increased susceptibility to bacterial infection induced morbidity/mortality ( J:158663 )

• highly susceptible to C. rodentium and all die at day 10-12 post infection

growth/size/body

weight loss ( J:158663 )

• exhibit weight loss post infection with C. rodentium

digestive/alimentary system

abnormal colon morphology ( J:158663 )

• severe disruption of the epithelial layer post infection with C. rodentium

colonic necrosis ( J:158663 )

• multifocal necrosis in colon post infection with C. rodentium

colitis ( J:158663 )

• inflammation, and edema post infection with C. rodentium

Genotype
MGI:3767256

cx2

• Allelic Composition: Rorc^tm1Litt/Rorc^tm1Litt; Tcra-J^tm3.1Krg/Tcra-J^tm3.1Krg
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac

hematopoietic system

abnormal T cell differentiation ( J:128344 )

• unlike in Rorc^tm1Litt homozygotes, primary rearrangement of 5' Jalpha segments is completely abolished (0.5% compared to 54% in Rorc^tm1Litt homozygotes) while rearrangement of the central Jalpha segments is increased (54.8% compared to 18.2% in Rorc^tm1Litt homozygotes)

immune system

abnormal T cell differentiation ( J:128344 )

• unlike in Rorc^tm1Litt homozygotes, primary rearrangement of 5' Jalpha segments is completely abolished (0.5% compared to 54% in Rorc^tm1Litt homozygotes) while rearrangement of the central Jalpha segments is increased (54.8% compared to 18.2% in Rorc^tm1Litt homozygotes)