Phenotypes associated with this allele
Allelic
Composition |
Smad5tm1Zuk/Smad5+
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Genetic
Background |
involves: 129S7/SvEvBrd * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad5tm1Zuk mutation
(1 available);
any
Smad5 mutation
(23 available)
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reproductive system
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• on average, 45 PGCs were detected in embyros with 0 to 5 somites (age matched wild-type mice had 65 PGCs)
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cellular
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• on average, 45 PGCs were detected in embyros with 0 to 5 somites (age matched wild-type mice had 65 PGCs)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad5tm1Zuk mutation
(1 available);
any
Smad5 mutation
(23 available)
Smad9tm2.1Rob mutation
(0 available);
any
Smad9 mutation
(32 available)
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mortality/aging
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• authors state phenotype is same as previously described for Smad5 null embryos; Smad9 deficiency does not exacerbate the Smad5 phenotype
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embryo
growth/size/body
cardiovascular system
nervous system
craniofacial
reproductive system
cellular
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad1tm1Rob mutation
(0 available);
any
Smad1 mutation
(32 available)
Smad5tm1Zuk mutation
(1 available);
any
Smad5 mutation
(23 available)
Smad9tm2.1Rob mutation
(0 available);
any
Smad9 mutation
(32 available)
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mortality/aging
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• no live born mice are obtained
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad1tm1Rob mutation
(0 available);
any
Smad1 mutation
(32 available)
Smad5tm1Zuk mutation
(1 available);
any
Smad5 mutation
(23 available)
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mortality/aging
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• no viable embryos are recovered at E13.5
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embryo
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• second branchial arches are absent or severely compromised in their development
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• third branchial arches are absent or severely compromised in their development
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• in the most severely affected mutants, mesoderm is underrepresented
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• expression of Nodal in embryos is disturbed in lateral plate mesoderm (LPM), showing compromised ability to activate left/right pathway in LPM
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• at E10.5 and 11.5, the most severely affected embryos (~33%) are poorly patterned along the anterior-posterior axis
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• in some embryos, rostral regions of CNS tissue are correctly specified, but cranial folds fail to fuse
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• somites are misaligned and fragmented in a high proportion of embryos
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• in many embryos, morphology is overtly normal except that allantois remains as a dense mass of tissue
• in some embryos the allantois is fused across the amnion
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• some embryos at E7.5 display ruffling of the visceral yolk sac
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cardiovascular system
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• heart chamber patterning and specification is severely disturbed
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• pronounced heart looping defects are observed in a high proportion of embryos
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• in some embryos, there is inversion of looping direction
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• in some embryos, looping is stalled, with heart tube remaining linear along ventral midline
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• anterior-posterior patterning of heart tube is abnormal
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nervous system
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• in the most severely affected embryos, there is a spectrum of anterior defects; embryos show absence of anterior-most neural tissue by Fgf8 expression, whereas midbrain-hindbrain isthmus is specified
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• most rostral regions of CNS are absent
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• in some embryos, rostral regions of CNS tissue are correctly specified, but cranial folds fail to fuse
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reproductive system
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• at the 10-15 somite stage, mutants show greatly reduced numbers of primordial germ cells (PGCs) compared to wild-type (wild-type 80-100 cells)
• at 20-25 somite stage, there are 200-300 germ cells in hindgut in wild-type, most mutants lack germ cells and remainder (~40%) show 10-fold fewer cells
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• in majority of embryos at the 20-25 somite stage, germ cells are absent
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craniofacial
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• second branchial arches are absent or severely compromised in their development
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• third branchial arches are absent or severely compromised in their development
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cellular
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• at the 10-15 somite stage, mutants show greatly reduced numbers of primordial germ cells (PGCs) compared to wild-type (wild-type 80-100 cells)
• at 20-25 somite stage, there are 200-300 germ cells in hindgut in wild-type, most mutants lack germ cells and remainder (~40%) show 10-fold fewer cells
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• in majority of embryos at the 20-25 somite stage, germ cells are absent
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Analysis Tools