Phenotypes associated with this allele

• Allele Symbol: Tg(Col2a1-PTHLH)1Ecw
• Allele Name: transgene insertion 1, Eleanor C Weir
• Allele ID: MGI:2182284
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pthlh^tm1Hmk/Pthlh^tm1Hmk Pth1r^tm1Hmk/Pth1r^tm2Hmk Tg(Bglap2-cre)1Kry/0 Tg(Col2a1-PTHLH)1Ecw/0	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 * FVB * SJL	MGI:3584347
cx2	Pthlh^tm1Hmk/Pthlh^tm1Hmk Pth1r^tm1Hmk/Pth1r^tm2Hmk Tg(Col2a1-PTHLH)1Ecw/0	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 * SJL	MGI:3584346
tg3	Tg(Col2a1-PTHLH)1Ecw/0	involves: C57BL/6 * SJL	MGI:3584163

Genotype
MGI:3584347

cn1

• Allelic Composition: Pthlh^tm1Hmk/Pthlh^tm1Hmk; Pth1r^tm1Hmk/Pth1r^tm2Hmk; Tg(Bglap2-cre)1Kry/0; Tg(Col2a1-PTHLH)1Ecw/0
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 * FVB * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

long tibia ( J:99641 )

• increase in tibia length

abnormal long bone epiphyseal plate morphology ( J:99641 )

• exhibit ectopically differentiated chondrocytes

abnormal chondrocyte differentiation ( J:99641 )

• eutopic hypertrophic chondrocyte differentiation is stimulated

limbs/digits/tail

long tibia ( J:99641 )

• increase in tibia length

Genotype
MGI:3584346

cx2

• Allelic Composition: Pthlh^tm1Hmk/Pthlh^tm1Hmk; Pth1r^tm1Hmk/Pth1r^tm2Hmk; Tg(Col2a1-PTHLH)1Ecw/0
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 * SJL

skeleton

short tibia ( J:99641 )

abnormal long bone epiphyseal plate morphology ( J:99641 )

• rescued growth plate lacks hypertrophic chondrocytes and columnar cells

abnormal bone ossification ( J:99641 )

• bone formation is suppressed

limbs/digits/tail

short tibia ( J:99641 )

immune system

abnormal bone marrow development ( J:99641 )

• bone marrow formation is suppressed

Genotype
MGI:3584163

tg3

• Allelic Composition: Tg(Col2a1-PTHLH)1Ecw/0
• Genetic Background: involves: C57BL/6 * SJL

Subgross analysis of skeletons of Tg(Col2a1-PTHLH)1Ecw/0 mice

growth/size/body

decreased body size ( J:76983 )

• small in size although growth rate is similar to controls

disproportionate dwarf ( J:76983 )

• short-limbed dwarfism

limbs/digits/tail

short limbs ( J:76983 )

short tail ( J:76983 )

skeleton

decreased length of long bones ( J:76983 )

• shortening and bowing of the long bones

abnormal vertebrae morphology ( J:76983 )

• vertebral length is reduced at 3 weeks of age

abnormal skeleton development ( J:76983 )

increased long bone epiphyseal plate size ( J:76983 )

• growth plates of long bones are thickened

delayed chondrocyte differentiation ( J:76983 )

• delay in chondrocyte differentiation

chondrodystrophy ( J:76983 )

• delay in endochondrial bone ossification and profoundly altered sequence of endochondral bone development

abnormal bone ossification ( J:76983 )

• sequence of endochondral bone development is altered such that chondrocyte hypertrophy begins at the periphery of long bones rather than in the middle and initially leads to formation of pseudogrowth plates

delayed endochondral bone ossification ( J:76983 )

• born with a cartilaginous endochondral skeleton

• ossification centers are not evident or are small and persist as cartilaginous primorida in many skeletal elements that form by endochondral ossification, however by 7 weeks of age, the delays in chondrocyte differentiation and ossification are largely corrected, leaving foreshortened and misshapen but histologically near-normal bones