Phenotypes associated with this allele

• Allele Symbol: Cd48^tm1Rsr
• Allele Name: targeted mutation 1, Hans Reiser
• Allele ID: MGI:2180770
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd48^tm1Rsr/Cd48^tm1Rsr	B6.129S4-Cd48^tm1Rsr	MGI:2662043

Genotype
MGI:2662043

hm1

• Allelic Composition: Cd48^tm1Rsr/Cd48^tm1Rsr
• Genetic Background: B6.129S4-Cd48^tm1Rsr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

increased CD4-positive, alpha-beta T cell number ( J:53718 )

• homozygotes display a slight increase in the fraction of CD4⁺ CD8^- T cells in thymus and spleen

• however, overall lymphoid development is grossly normal, as determined by dual color immunofluorescence and flow cytometry

abnormal T cell activation ( J:53718 )

• homozygotes display a marked defect in CD4⁺ T cell activation mediated through the T cell receptor/CD3 complex

decreased T cell proliferation ( J:53718 )

• homozygotes exhibit impaired T cell proliferation in response to stimulation with lectins, anti-CD3 antibodies, and alloantigens; however, no proliferative defects are detected when mutant splenocytes are activated with LPS (a B cell mitogen)

• the proliferative responses of mutant splenocytes to Con A and anti-CD3 mAb are reduced by ~20%-50%, while responses to phytohemagglutinin (PHA) are almost completely abolished

• in addition, the proliferative responses of highly-purified mutant CD4⁺ T cells to TCR stimulation with either anti-CD3 mAb and phorbol 12-myristate 13-acetate (PMA) or with Con A and PMA are abrogated, whereas responses to a mitogenic combination of calcium ionophore and PMA remain unaffected

immune system

increased CD4-positive, alpha-beta T cell number ( J:53718 )

• homozygotes display a slight increase in the fraction of CD4⁺ CD8^- T cells in thymus and spleen

• however, overall lymphoid development is grossly normal, as determined by dual color immunofluorescence and flow cytometry

abnormal T cell activation ( J:53718 )

• homozygotes display a marked defect in CD4⁺ T cell activation mediated through the T cell receptor/CD3 complex

decreased T cell proliferation ( J:53718 )

• homozygotes exhibit impaired T cell proliferation in response to stimulation with lectins, anti-CD3 antibodies, and alloantigens; however, no proliferative defects are detected when mutant splenocytes are activated with LPS (a B cell mitogen)

• the proliferative responses of mutant splenocytes to Con A and anti-CD3 mAb are reduced by ~20%-50%, while responses to phytohemagglutinin (PHA) are almost completely abolished

• in addition, the proliferative responses of highly-purified mutant CD4⁺ T cells to TCR stimulation with either anti-CD3 mAb and phorbol 12-myristate 13-acetate (PMA) or with Con A and PMA are abrogated, whereas responses to a mitogenic combination of calcium ionophore and PMA remain unaffected

cellular

decreased T cell proliferation ( J:53718 )

• homozygotes exhibit impaired T cell proliferation in response to stimulation with lectins, anti-CD3 antibodies, and alloantigens; however, no proliferative defects are detected when mutant splenocytes are activated with LPS (a B cell mitogen)

• the proliferative responses of mutant splenocytes to Con A and anti-CD3 mAb are reduced by ~20%-50%, while responses to phytohemagglutinin (PHA) are almost completely abolished

• in addition, the proliferative responses of highly-purified mutant CD4⁺ T cells to TCR stimulation with either anti-CD3 mAb and phorbol 12-myristate 13-acetate (PMA) or with Con A and PMA are abrogated, whereas responses to a mitogenic combination of calcium ionophore and PMA remain unaffected