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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cd247tm1Mhu
targeted mutation 1, Manley Huang
MGI:2180467
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cd247tm1Mhu/Cd247tm1Mhu involves: 129S7/SvEvBrd * C57BL/6 MGI:3818443
cx2
 		Cd247tm1Mhu/Cd247tm1Mhu
Fcer1gtm1Rav/Fcer1gtm1Rav 		involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 MGI:3818419


Genotype
MGI:3818443
hm1
Allelic
Composition		 Cd247tm1Mhu/Cd247tm1Mhu
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd247tm1Mhu mutation (0 available); any Cd247 mutation (11 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:15579 )
N
• despite the reduction in T cell differentiation in the thymus, the numbers and ratios of CD4+ or CD8+ T cells in the spleen and lymph nodes, VDJ rearrangement, and the numbers and function of NK cells are normal
decreased T cell proliferation ( J:15579 )
• intraepithelial lymphocytes fail to proliferate in response to stimulation with anti-CD3 antibodies and ConA unlike heterozygous cells
decreased splenocyte proliferation ( J:15579 )
• TCR/CD3very low CD4+ and CD8+ single positive splenocytes fail to proliferate in response to anti-CD3 antibodies and ConA unlike heterozygous cells
enlarged thymus cortex ( J:15579 )
• the size of the cortex is enlarged at the expense of the medulla
small thymus medulla ( J:15579 )
• the medulla is restricted to small regions unlike in heterozygous mice
decreased thymocyte number ( J:15579 )
• at 8 to 12 weeks, thymocyte number is decreased 5- to 10-fold compared to in heterozygous mice
abnormal T cell differentiation ( J:15579 )
• mice exhibit a delayed and reduced differentiation of double negative T cells into double positive TCR/CD3hi T cells in the thymus compared to in heterozygous mice instead generating TCR/CD3very low CD4+ and CD8+ single positive T cells
• however, intraepithelial lymphocytes express normal levels of TCR/CD3
increased double-negative T cell number ( J:15579 )
• the percentage of double negative cells in the thymus is more than in heterozygous mice
• however, the absolute number of double negative cells is normal
decreased double-positive T cell number ( J:15579 )
• the percentage of double positive cells in the thymus is decreased compared to in heterozygotes
decreased CD4-positive, alpha-beta T cell number ( J:15579 )
• the percentage of CD4+ single positive cells in the thymus is decreased compared to in heterozygous mice mice that are indistinguishable from wild-type mice
decreased CD8-positive, alpha-beta T cell number ( J:15579 )
• the percentage of CD8+ single positive cells in the thymus is decreased compared to in heterozygous mice

hematopoietic system
decreased T cell proliferation ( J:15579 )
• intraepithelial lymphocytes fail to proliferate in response to stimulation with anti-CD3 antibodies and ConA unlike heterozygous cells
decreased splenocyte proliferation ( J:15579 )
• TCR/CD3very low CD4+ and CD8+ single positive splenocytes fail to proliferate in response to anti-CD3 antibodies and ConA unlike heterozygous cells
enlarged thymus cortex ( J:15579 )
• the size of the cortex is enlarged at the expense of the medulla
small thymus medulla ( J:15579 )
• the medulla is restricted to small regions unlike in heterozygous mice
decreased thymocyte number ( J:15579 )
• at 8 to 12 weeks, thymocyte number is decreased 5- to 10-fold compared to in heterozygous mice
abnormal T cell differentiation ( J:15579 )
• mice exhibit a delayed and reduced differentiation of double negative T cells into double positive TCR/CD3hi T cells in the thymus compared to in heterozygous mice instead generating TCR/CD3very low CD4+ and CD8+ single positive T cells
• however, intraepithelial lymphocytes express normal levels of TCR/CD3
increased double-negative T cell number ( J:15579 )
• the percentage of double negative cells in the thymus is more than in heterozygous mice
• however, the absolute number of double negative cells is normal
decreased double-positive T cell number ( J:15579 )
• the percentage of double positive cells in the thymus is decreased compared to in heterozygotes
decreased CD4-positive, alpha-beta T cell number ( J:15579 )
• the percentage of CD4+ single positive cells in the thymus is decreased compared to in heterozygous mice mice that are indistinguishable from wild-type mice
decreased CD8-positive, alpha-beta T cell number ( J:15579 )
• the percentage of CD8+ single positive cells in the thymus is decreased compared to in heterozygous mice

cellular
decreased T cell proliferation ( J:15579 )
• intraepithelial lymphocytes fail to proliferate in response to stimulation with anti-CD3 antibodies and ConA unlike heterozygous cells
decreased splenocyte proliferation ( J:15579 )
• TCR/CD3very low CD4+ and CD8+ single positive splenocytes fail to proliferate in response to anti-CD3 antibodies and ConA unlike heterozygous cells

endocrine/exocrine glands
enlarged thymus cortex ( J:15579 )
• the size of the cortex is enlarged at the expense of the medulla
small thymus medulla ( J:15579 )
• the medulla is restricted to small regions unlike in heterozygous mice
decreased thymocyte number ( J:15579 )
• at 8 to 12 weeks, thymocyte number is decreased 5- to 10-fold compared to in heterozygous mice

growth/size/body
enlarged spleen ( J:15579 )




Genotype
MGI:3818419
cx2
Allelic
Composition		 Cd247tm1Mhu/Cd247tm1Mhu
Fcer1gtm1Rav/Fcer1gtm1Rav
Genetic
Background		 involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd247tm1Mhu mutation (0 available); any Cd247 mutation (11 available)
Fcer1gtm1Rav mutation (8 available); any Fcer1g mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal maternal decidual layer morphology ( J:100668 )
• embryo implantation sites are hypercellular compared to in wild-type mice
• at E10 and E12, the mesometrial lymphoid aggregate of pregnancy is enlarged compared to in wild-type mice
abnormal decidua basalis morphology ( J:100668 )
• at E10, the deciduas basalis is larger compared to in wild-type mice
decreased uterine NK cell number ( J:100668 )
• the number of uterine NK cells at E10 in the mesometrial lymphoid aggregate of pregnancy and at E12 in the deciduas basalis is reduced compared to in wild-type mice
abnormal uterine spiral artery morphology ( J:100668 )
• at E10 and E12, the decidual spiral arterial vessel to lumen area is increased compared to in wild-type mice
abnormal uterine spiral artery remodeling ( J:100668 )
• at E12, thick-walled arteries are prominent in embryo implantation site unlike in wild-type mice
• uterine NK cells fail to modify the spiral arteries of the decidua as in wild-type mice
reduced fertility ( J:100668 )
• fewer mice become pregnant following mating compared to wild-type mice
• early post-implantation fetal loss (E6 and E8) is greater than in wild-type mice

embryo
abnormal maternal decidual layer morphology ( J:100668 )
• embryo implantation sites are hypercellular compared to in wild-type mice
• at E10 and E12, the mesometrial lymphoid aggregate of pregnancy is enlarged compared to in wild-type mice
abnormal decidua basalis morphology ( J:100668 )
• at E10, the deciduas basalis is larger compared to in wild-type mice
decreased uterine NK cell number ( J:100668 )
• the number of uterine NK cells at E10 in the mesometrial lymphoid aggregate of pregnancy and at E12 in the deciduas basalis is reduced compared to in wild-type mice
abnormal uterine spiral artery morphology ( J:100668 )
• at E10 and E12, the decidual spiral arterial vessel to lumen area is increased compared to in wild-type mice

immune system
decreased uterine NK cell number ( J:100668 )
• the number of uterine NK cells at E10 in the mesometrial lymphoid aggregate of pregnancy and at E12 in the deciduas basalis is reduced compared to in wild-type mice

hematopoietic system
decreased uterine NK cell number ( J:100668 )
• the number of uterine NK cells at E10 in the mesometrial lymphoid aggregate of pregnancy and at E12 in the deciduas basalis is reduced compared to in wild-type mice

endocrine/exocrine glands
decreased uterine NK cell number ( J:100668 )
• the number of uterine NK cells at E10 in the mesometrial lymphoid aggregate of pregnancy and at E12 in the deciduas basalis is reduced compared to in wild-type mice




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Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory