Phenotypes associated with this allele

• Allele Symbol: Casp3^tm1Mak
• Allele Name: targeted mutation 1, Tak W Mak
• Allele ID: MGI:2178778
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Casp3^tm1Mak/Casp3^tm1Mak	B6.129P2-Casp3^tm1Mak	MGI:3623576
cx2	Casp3^tm1Mak/Casp3^tm1Mak Tg(TcrLCMV)327Sdz/0	B6.Cg-Casp3^tm1Mak Tg(TcrLCMV)327Sdz	MGI:3623578

Genotype
MGI:3623576

hm1

• Allelic Composition: Casp3^tm1Mak/Casp3^tm1Mak
• Genetic Background: B6.129P2-Casp3^tm1Mak

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:97650 )

• about 50% of homozygotes survive to adulthood

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:97650 )

• after injection with streptozocin for 5 consecutive days, blood glucose in homozygotes remains normoglycemic for the duration of the study and pancreas samples showed normal islets

immune system

decreased inflammatory response ( J:97650 , J:179279 )

• islets from homozygous mice show no infiltration at any time after streptozocin treatment compared to heterozygotes (J:97650)

• however, cytokine concentrations, hypothermia, and mortality after TNF treatment are similar to wild-type controls (J:179279)

• after treatment with TNF unlike in wild-type mice swollen villi, an increase in infiltrates, and high levels of apoptosis are almost absent (J:179279)

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	type 1 diabetes mellitus	DOID:9744	OMIM:222100	J:97650

Genotype
MGI:3623578

cx2

• Allelic Composition: Casp3^tm1Mak/Casp3^tm1Mak; Tg(TcrLCMV)327Sdz/0
• Genetic Background: B6.Cg-Casp3^tm1Mak Tg(TcrLCMV)327Sdz

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:97650 )

• after injection with streptozocin for 5 consecutive days, blood glucose in caspase-3-homozygous transgenic mice remains normoglycemic for the duration of the study

cellular

decreased cell proliferation ( J:97650 )

• adoptive transfer of labeled Tg(TcrLCMV)327Sdz-transgene positive T cells, examination of pancreatic draining lymph nodes showed that only 45% of the transferred T cells had undergone proliferation compared to 62% in caspase-3-heterozygous transgenic recipients

immune system

abnormal CD8-positive, alpha beta T cell morphology ( J:97650 )

• in CD8+ T cells isolated from pancreatic draining or non-draining lymph nodes of streptozocin-treated mice, 32% of the CD8+ cells are CD69hi positive and 9% respectively, compared to corresponding values of 3% and 5% respectively in caspase-3-heterozygous transgenic mice

hematopoietic system

abnormal CD8-positive, alpha beta T cell morphology ( J:97650 )

• in CD8+ T cells isolated from pancreatic draining or non-draining lymph nodes of streptozocin-treated mice, 32% of the CD8+ cells are CD69hi positive and 9% respectively, compared to corresponding values of 3% and 5% respectively in caspase-3-heterozygous transgenic mice