Phenotypes associated with this allele

• Allele Symbol: Mycn^tm1Jrt
• Allele Name: targeted mutation 1, Janet Rossant
• Allele ID: MGI:2178220
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mycn^tm1Jrt/Mycn^tm1Jrt	involves: 129S2/SvPas * C57BL/6J	MGI:2180007
ht2	Mycn^tm1Jrt/Mycn^tm1Par	involves: 129S2/SvPas * C57BL/6J	MGI:3620016

Genotype
MGI:2180007

hm1

• Allelic Composition: Mycn^tm1Jrt/Mycn^tm1Jrt
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:973 )

• animals die shortly after birth

behavior/neurological

no spontaneous movement ( J:973 )

• animals do not move after birth

respiratory system

abnormal lung morphology ( J:973 )

• thin septa, thin and fragile outer surface

respiratory distress ( J:973 )

• animals gasp for breath after birth

Genotype
MGI:3620016

ht2

• Allelic Composition: Mycn^tm1Jrt/Mycn^tm1Par
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:15281 )

• compound heterozygotes start dying at ~E12.5, when 12 out of 42 mutants are found to be dead and necrotic

• no live compound heterozygotes are obtained after E14.5

cardiovascular system

abnormal vein morphology ( J:15281 )

• at E12.5, compound heterozygotes with an edematous neck area exhibit massively dilated jugular veins, indicating cardiac dysfunction

• however, no other major veins are shown to exhibit significant dilation

thin myocardium compact layer ( J:15281 )

• at E12.5, compound heterozygotes show a thin subepicardial layer in the ventricular myocardium; the atrial myocardium and inner trabecular layer of ventricles are less severly affected

• at E12.5, the mutant subepicardial ("compact") layer has failed to grow and is as thick as at E10.5

thin myocardium ( J:15281 )

• at E12.5, compound heterozygotes display a thinned myocardium relative to wild-type mice

small heart ( J:15281 )

decreased fetal cardiomyocyte proliferation ( J:15281 )

• at E12.5 and E14.5, compound heterozygotes fail to exhibit proliferation in the subepicardial ("compact") layer of the ventricular myocardium

congestive heart failure ( J:15281 )

respiratory system

impaired branching involved in bronchus morphogenesis ( J:15281 )

• at E12.5, compound heterozygotes display an exacerbated branching defect in the lung epithelium relative to Mycn^tm1Jrt homozygotes, as shown by absence of tertiary branching

homeostasis/metabolism

edema ( J:15281 )

• at E12.5, 23 out of 42 compound heterozygotes display a large edema in the neck area

• all compound heterozygotes surviving to E14.5 show an edematous neck area

embryo

decreased embryo size ( J:15281 )

• at E12.5, 2 out of 42 compound heterozygotes are slightly smaller than wild-type embryos

renal/urinary system

small kidney ( J:15281 )

• compound heterozygotes that survive to E14.5 exhibit small but structurally normal kidneys relative to wild-type mice

growth/size/body

decreased embryo size ( J:15281 )

• at E12.5, 2 out of 42 compound heterozygotes are slightly smaller than wild-type embryos

decreased fetal size ( J:15281 )

• all compound heterozygotes surviving to E14.5 are smaller than wild-type embryos

muscle

thin myocardium compact layer ( J:15281 )

• at E12.5, compound heterozygotes show a thin subepicardial layer in the ventricular myocardium; the atrial myocardium and inner trabecular layer of ventricles are less severly affected

• at E12.5, the mutant subepicardial ("compact") layer has failed to grow and is as thick as at E10.5

thin myocardium ( J:15281 )

• at E12.5, compound heterozygotes display a thinned myocardium relative to wild-type mice

decreased fetal cardiomyocyte proliferation ( J:15281 )

• at E12.5 and E14.5, compound heterozygotes fail to exhibit proliferation in the subepicardial ("compact") layer of the ventricular myocardium

nervous system

nervous system phenotype ( J:15281 )

N • unlike Mycn^tm1Par homozygotes, compound heterozygotes do NOT exhibit reduced cranial or spinal ganglia

reproductive system

reproductive system phenotype ( J:15281 )

N • unlike Mycn^tm1Par homozygotes, compound heterozygotes do NOT exhibit reduced genital ridges

cellular

decreased fetal cardiomyocyte proliferation ( J:15281 )

• at E12.5 and E14.5, compound heterozygotes fail to exhibit proliferation in the subepicardial ("compact") layer of the ventricular myocardium