Phenotypes associated with this allele

• Allele Symbol: Map2k1^{Gt(ROSABetageo)J2Chrn}
• Allele Name: gene trap J2, Jean Charron
• Allele ID: MGI:2178132
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Map2k1^Gt(ROSABetageo)J2Chrn/Map2k1^Gt(ROSABetageo)J2Chrn	involves: 129/Sv * 129S/SvEv * C57BL/6J * SJL	MGI:3580657
hm2	Map2k1^Gt(ROSABetageo)J2Chrn/Map2k1^Gt(ROSABetageo)J2Chrn	involves: 129/Sv * C57BL/6J * SJL	MGI:3714924

Genotype
MGI:3580657

hm1

• Allelic Composition: Map2k1^{Gt(ROSABetageo)J2Chrn}/Map2k1^{Gt(ROSABetageo)J2Chrn}
• Genetic Background: involves: 129/Sv * 129S/SvEv * C57BL/6J * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:54197 )

• two thirds of homozygous embryos were seriously underdeveloped or dead by E10.5

embryo

embryo tissue necrosis ( J:54197 )

• necrosis was observed in various tissues at E10.5

decreased embryo size ( J:54197 )

• one third of homozygous embryos were undersized at E10.5

abnormal spongiotrophoblast layer morphology ( J:54197 )

• poorly defined spongiotrophoblast layer

abnormal placenta labyrinth morphology ( J:54197 )

• compact

abnormal placental labyrinth vasculature morphology ( J:54197 )

• few blood vessels present

pale yolk sac ( J:54197 )

• apparently devoid of circulation

• few blood cells and vessels distended

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:54197 )

• few blood vessels present

abnormal angiogenesis ( J:54197 )

• angiogenesis was defective although early stages and vasculogenesis were normal

abnormal vascular endothelial cell migration ( J:54197 )

• vascular endothelial cells remain primarily in the chorioallantoic region with little penetration of the labyrinthine layer

growth/size/body

decreased embryo size ( J:54197 )

• one third of homozygous embryos were undersized at E10.5

cellular

embryo tissue necrosis ( J:54197 )

• necrosis was observed in various tissues at E10.5

abnormal cell migration ( J:54197 )

• fibronectin induced cell migration fails

abnormal vascular endothelial cell migration ( J:54197 )

• vascular endothelial cells remain primarily in the chorioallantoic region with little penetration of the labyrinthine layer

Genotype
MGI:3714924

hm2

• Allelic Composition: Map2k1^{Gt(ROSABetageo)J2Chrn}/Map2k1^{Gt(ROSABetageo)J2Chrn}
• Genetic Background: involves: 129/Sv * C57BL/6J * SJL

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:112223 )

• mice die at E10.5

embryo

abnormal placenta morphology ( J:112223 )

• at E9.5 and E10.5, placenta proliferation is halved

abnormal placenta labyrinth morphology ( J:112223 )

• apoptosis in the placental labyrinth is increased 5-fold at E8.5 and E9.5 and 10-fold at E10.5

small placenta ( J:112223 )

• at E9.5