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Allele Symbol Allele Name Allele ID |
Egftm1Dle targeted mutation 1, David C Lee MGI:2176530 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• most homozygous villous goblet cells stained less robustly with mucicarmine, suggesting they are producing lower amounts of mucin
• the number of villous goblet cells was decreased 32% relative to wild-type villi
• not observed in mice homozygous for any pair-wise combination of these three genes
• reduced levels of trefoil production, a peptide family critical for mucosal defense and ulcer healing
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• the weight/length ratio of the small intestine was reduced 37% in triple null pups; functional maturation at weaning age was not affected
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• fewer crypt cells were proliferating in homozygous ileum compared to wild-type
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• spontaneous lesions affecting mucosal integrity observed in 50% of samples; lesions varied from mild erosions to occasional perforations; not observed in mice homozygous for any pair-wise combination of these three genes
• more susceptible to induced duodenal lesions; cysteamine treatment produced more severe lesions than in wild-type mice
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• stunted villi with jagged or sheared tips; the average villus height in mutant ileum was reduced 47% relative to wild-type at three weeks, but only 11% at 5 weeks
• the muscularis mucosa layer was reduced in thickness at three weeks of age
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• fewer crypt cells were proliferating in homozygous ileum compared to wild-type
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• ducts in virgin pubescent glands hardly extended beyond the rudimentary anlage and only a few terminal end buds were observed at 6 weeks
• at 12 weeks, ductal trees were underdeveloped and had persistent terminal end buds
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• during pregnancy, mammary glands exhibited defects in lobuloalveolar development; alveoli appear small and dense instead of engorged with fat droplets at day 18 of pregnancy
• on day 2 of lactation, alveoli are compressed and overcrowded, and exhibit increased epithelial density with irregular, compact alveolar morphology observed
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• mutant mammary glands could not sustain lactation and expression of milk protein genes was decreased
• pups did not thrive due to decreased milk production of dams
• lactation improved with aged mothers
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• a 40% reduction in the body weight of pups by 3 weeks was observed; partially attenuated by cross-fostering to wild-type dams
• by 7 weeks, weights were 15% reduced relative to wild-type, suggesting that a maternal lactation defect was partially responsible for the weight loss and this is overcome on a chow diet
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• accelerated with aging
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• postnatal growth retardation of triple null pups was observed independent of maternal genotype
• more severe growth defects were seen when the mother was also triple null, and less severe growth defects were seen when the mother was wild-type
• control pups born to triple null mothers also exhibited growth retardation probably due to decreased milk production
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• during pregnancy, mammary glands exhibited defects in lobuloalveolar development; alveoli appear small and dense instead of engorged with fat droplets at day 18 of pregnancy
• on day 2 of lactation, alveoli are compressed and overcrowded, and exhibit increased epithelial density with irregular, compact alveolar morphology observed
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• incidence noted as more frequent (80-90%) than the single Tgfatm1Unc homozygous mutants (40-50%)
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• incidence noted as more frequent (80-90%) than the single Tgfatm1Unc homozygous mutants (40-50%)
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• incidence noted as more frequent (80-90%) than the single Tgfatm1Unc homozygous mutants (40-50%)
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• accelerated with aging
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• ducts in virgin pubescent glands hardly extended beyond the rudimentary anlage and only a few terminal end buds were observed at 6 weeks
• at 12 weeks, ductal trees were underdeveloped and had persistent terminal end buds
|
|
|
• during pregnancy, mammary glands exhibited defects in lobuloalveolar development; alveoli appear small and dense instead of engorged with fat droplets at day 18 of pregnancy
• on day 2 of lactation, alveoli are compressed and overcrowded, and exhibit increased epithelial density with irregular, compact alveolar morphology observed
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• mutant mammary glands could not sustain lactation and expression of milk protein genes was decreased
• pups did not thrive due to decreased milk production of dams
• lactation improved with aged mothers
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• accelerated with aging
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• accelerated with aging
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• accelerated with aging
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• most homozygous villous goblet cells stained less robustly with mucicarmine, suggesting they are producing lower amounts of mucin
• the number of villous goblet cells was decreased 32% relative to wild-type villi
• not observed in mice homozygous for any pair-wise combination of these three genes
• reduced levels of trefoil production, a peptide family critical for mucosal defense and ulcer healing
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• partial penetrance similar to that in Tgfatm1Unc single homozygotes
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• similar to that in Tgfatm1Unc single homozygotes
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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