Phenotypes associated with this allele

• Allele Symbol: Tie1^tm1Sato
• Allele Name: targeted mutation 1, Thomas N Sato
• Allele ID: MGI:2159359
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tie1^tm1Sato/Tie1^tm1Sato	either: (involves: 129S1/Sv * 129T2/SvEmsJ) or (involves: 129S1/Sv * C57BL/6J)	MGI:3612283
hm2	Tie1^tm1Sato/Tie1^tm1Sato	involves: 129S1/Sv * C57BL/6J	MGI:3612284

Genotype
MGI:3612283

hm1

• Allelic Composition: Tie1^tm1Sato/Tie1^tm1Sato
• Genetic Background: either: (involves: 129S1/Sv * 129T2/SvEmsJ) or (involves: 129S1/Sv * C57BL/6J)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:26844 )

• homozygotes die immediately after birth

embryonic lethality during organogenesis, incomplete penetrance ( J:26844 )

• 4 of 27 embryos are dead at E13.5

respiratory system

pulmonary edema ( J:26844 )

• seen at birth

respiratory failure ( J:26844 )

• alveoli are unexpanded possibly a result of pulmonary edema

cardiovascular system

abnormal vascular endothelial cell morphology ( J:26844 )

• vascular patterning appears normal; however, at E13.5 vascular endothelial cells that are abnormally electron light are seen

abnormal endocardium morphology ( J:26844 )

• more immature endocardial cells are seen compared to wild-type mice

small heart ( J:26844 )

• the heart is small at birth but appears grossly morphologically normal at E13.5

hemorrhage ( J:26844 )

• at E13.5 subcutaneous hemorrhage is seen on the back of dead embryos (4 of 27)

• at birth, hemorrhages are seen in the tail tip, and in some mice in the toe tips and sagital sinus of the head and back, also internal hemorrhages are also seen in many organs including the intestine and stomach

• erythrocytes extravasate through the endothelial cells not through the tight junctions between these cells

homeostasis/metabolism

intestinal edema ( J:26844 )

• seen at birth

pulmonary edema ( J:26844 )

• seen at birth

skin edema ( J:26844 )

• subcutaneous edema of the back is seen at E13.5 in living embryos and at birth

digestive/alimentary system

intestinal edema ( J:26844 )

• seen at birth

integument

skin edema ( J:26844 )

• subcutaneous edema of the back is seen at E13.5 in living embryos and at birth

Genotype
MGI:3612284

hm2

• Allelic Composition: Tie1^tm1Sato/Tie1^tm1Sato
• Genetic Background: involves: 129S1/Sv * C57BL/6J

cardiovascular system

abnormal vascular branching morphogenesis ( J:79305 )

• at E15.5 and E18.5 a 1.5- to 2-fold increase in the number of vessels is seen in the brain, kidney, skin, lungs, intestine, and atrium of the heart

abnormal vascular endothelial cell development ( J:79305 )

• more vascular endothelial cells are hyperactive producing more extensions and filopodia, these connect to tissue folds that eventually divide the vessels, these cells are confined to a smaller subset of vessels in wild-type mice

• intra- and transcellular holes are common in these hyperactive cells

abnormal heart atrium morphology ( J:79305 )

• the papillary muscles are smaller and the atrial wall is thinner

abnormal endocardium morphology ( J:79305 )

• the endocardium surrounding papillary muscles has a more complex structure

digestive/alimentary system

abnormal small intestine morphology ( J:79305 )

• the capillary network in the small intestine is more complex and overexpanded compared to wild-type mice

renal/urinary system

abnormal renal glomerulus morphology ( J:79305 )

• the number of glomeruli is increased

respiratory system

abnormal pulmonary alveolus morphology ( J:79305 )

• the diameter of the alveoli is decreased

thick pulmonary interalveolar septum ( J:79305 )

• a thickening and increase in the number of septa separating the alveoli are seen