Phenotypes associated with this allele

• Allele Symbol: Atoh7^tm2Gan
• Allele Name: targeted mutation 2, Lin Gan
• Allele ID: MGI:2159014
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Atoh7^tm2Gan/Atoh7^tm2Gan	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:4867682
hm2	Atoh7^tm2Gan/Atoh7^tm2Gan	involves: 129S/SvEv * C57BL/6J	MGI:3707984
hm3	Atoh7^tm2Gan/Atoh7^tm2Gan	Not Specified	MGI:5472142
ht4	Atoh7^tm1Gan/Atoh7^tm2Gan	involves: 129S/SvEv * C57BL/6J	MGI:3707983
cn5	Atoh7^tm2Gan/Atoh7^tm2Gan Rest^tm1.1Whk/Rest^tm1.1Whk Tg(Six3-cre)69Frty/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:4867681
cx6	Atoh7^tm2Gan/Atoh7^tm2Gan Neurod1^tm1.1(Atoh7)Whk/Neurod1^tm1.1(Atoh7)Whk	Not Specified	MGI:5472141

Genotype
MGI:4867682

hm1

• Allelic Composition: Atoh7^tm2Gan/Atoh7^tm2Gan
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased retina ganglion cell number ( J:167071 )

• at E13.5

vision/eye

decreased retina ganglion cell number ( J:167071 )

• at E13.5

Genotype
MGI:3707984

hm2

• Allelic Composition: Atoh7^tm2Gan/Atoh7^tm2Gan
• Genetic Background: involves: 129S/SvEv * C57BL/6J

reproductive system

reproductive system phenotype ( J:67382 )

N • mice are fertile

vision/eye

abnormal optic nerve morphology ( J:67382 )

• very thin

decreased optic chiasm size ( J:67382 )

• very thin

abnormal retina morphology ( J:67382 )

• the overall structure of retina and number of photoreceptor cells is normal

abnormal retina ganglion cell morphology ( J:67382 )

• retinal ganglion cells fail to differentiate as shown by the loss of Pou4f1+ and reduction of Ngfr+ cells

• approximately 97% of Pou4f2+ cells are lost

• 80% reduction in the SMI-32 positive processes of axons from large ganglion cells and the remaining 20% of SMI-32 positive processes of axons have abnormally curved axon bundles

decreased retina ganglion cell number ( J:67382 )

• greater than 80% reduction in the number of retinal ganglion cells

abnormal retina nerve fiber layer morphology ( J:67382 )

• absence of a defined nerve fiber layer

decreased total retina thickness ( J:67382 )

• retinas are approximately 15 to 20% thinner than control retinas

nervous system

abnormal retina ganglion cell morphology ( J:67382 )

• retinal ganglion cells fail to differentiate as shown by the loss of Pou4f1+ and reduction of Ngfr+ cells

• approximately 97% of Pou4f2+ cells are lost

• 80% reduction in the SMI-32 positive processes of axons from large ganglion cells and the remaining 20% of SMI-32 positive processes of axons have abnormally curved axon bundles

decreased retina ganglion cell number ( J:67382 )

• greater than 80% reduction in the number of retinal ganglion cells

abnormal optic nerve morphology ( J:67382 )

• very thin

decreased optic chiasm size ( J:67382 )

• very thin

Genotype
MGI:5472142

hm3

• Allelic Composition: Atoh7^tm2Gan/Atoh7^tm2Gan
• Genetic Background: Not Specified

vision/eye

abnormal rod electrophysiology ( J:194077 )

• hardly show either the positive or negative scotopic response waves in a dark adapted electroretinogram in response to weak stimulus

• positive scotopic threshold response amplitudes are significantly lower than in controls

• negative scotopic threshold responses are significantly lower than in controls

• b-wave amplitudes are lower than in controls

Genotype
MGI:3707983

ht4

• Allelic Composition: Atoh7^tm1Gan/Atoh7^tm2Gan
• Genetic Background: involves: 129S/SvEv * C57BL/6J

vision/eye

abnormal retina ganglion cell morphology ( J:67382 )

• at E15.5, the ganglion cell layer contains very few differentiated ganglion cells

• however, at E11 and E15.5 the number of progenitor retinal ganglion cells are similar to that in heterozygotes and there is no evidence of increased apoptosis at E12.5 to E19

abnormal retina nerve fiber layer morphology ( J:67382 )

• absence of nerve fiber layer

nervous system

abnormal retina ganglion cell morphology ( J:67382 )

• at E15.5, the ganglion cell layer contains very few differentiated ganglion cells

• however, at E11 and E15.5 the number of progenitor retinal ganglion cells are similar to that in heterozygotes and there is no evidence of increased apoptosis at E12.5 to E19

Genotype
MGI:4867681

cn5

• Allelic Composition: Atoh7^tm2Gan/Atoh7^tm2Gan; Rest^tm1.1Whk/Rest^tm1.1Whk; Tg(Six3-cre)69Frty/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * DBA/2

vision/eye

abnormal retina morphology ( J:167071 )

• mis-patterning of the retina is more severe than in mutant mice wild-type for Atoh7

abnormal retina development ( J:167071 )

• few ganglion cells are detected in the nascent retinal ganglion layer at E13.5 despite many being generated in the neuroblast layer suggesting a defect in migration and maturation

• many dying cells are present in the retinal ganglion cell layer at E13.5

Genotype
MGI:5472141

cx6

• Allelic Composition: Atoh7^tm2Gan/Atoh7^tm2Gan; Neurod1^{tm1.1(Atoh7)Whk}/Neurod1^{tm1.1(Atoh7)Whk}
• Genetic Background: Not Specified

vision/eye

decreased retina ganglion cell number ( J:194077 )

• retinas have 28% of the Isl1-positive RGCs compared to wild-type mice at E13 indicating a partial restoration of the Atoh7 null phenotype

• retinas have about 25% of the number of retinal ganglion cells as in wild-type mice

• however, cells are generated evenly across the dorsal-ventral and nasal-temporal axes

abnormal optic nerve morphology ( J:194077 )

• diameters are 20 to 40% that of wild-type optic nerves

• retinal ganglion cell axons form, fasciculate, and extend into the optic disk indicating a partial rescue of the Atoh7 null phenotype

abnormal rod electrophysiology ( J:194077 )

• dark adapted electroretinogram responses to the weakest stimuli are similar in waveform but smaller than in controls

nervous system

abnormal sensory neuron innervation pattern ( J:194077 )

• retinal ganglion cell axons project to slightly more anterior areas in the superior colliculus

decreased retina ganglion cell number ( J:194077 )

• retinas have 28% of the Isl1-positive RGCs compared to wild-type mice at E13 indicating a partial restoration of the Atoh7 null phenotype

• retinas have about 25% of the number of retinal ganglion cells as in wild-type mice

• however, cells are generated evenly across the dorsal-ventral and nasal-temporal axes

abnormal optic nerve morphology ( J:194077 )

• diameters are 20 to 40% that of wild-type optic nerves

• retinal ganglion cell axons form, fasciculate, and extend into the optic disk indicating a partial rescue of the Atoh7 null phenotype