Phenotypes associated with this allele

• Allele Symbol: Gpx1^tm1Ysh
• Allele Name: targeted mutation 1, Ye-Shih Ho
• Allele ID: MGI:2158814
• Summary: 10 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gpx1^tm1Ysh/Gpx1^tm1Ysh	B6.129-Gpx1^tm1Ysh	MGI:2652385
hm2	Gpx1^tm1Ysh/Gpx1^tm1Ysh	involves: 129S1/Sv * 129X1/SvJ	MGI:3653634
hm3	Gpx1^tm1Ysh/Gpx1^tm1Ysh	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2176730
ht4	Gpx1^tm1Ysh/Gpx1^+	involves: 129S1/Sv * 129X1/SvJ	MGI:3653633
cx5	Gpx1^tm1Ysh/Gpx1^tm1Ysh Prkn^tm1Shn/Prkn^tm1Shn Park7^tm1Shn/Park7^tm1Shn	B6.Cg-Park7^tm1Shn Gpx1^tm1Ysh Prkn^tm1Shn	MGI:5527284
cx6	Gpx1^tm1Ysh/Gpx1^tm1Ysh Gpx3^tm1Hjco/Gpx3^tm1Hjco	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:4456209
cx7	Gpx1^tm1Ysh/Gpx1^+ Gpx2^tm2Coh/Gpx2^tm2Coh	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2652741
cx8	Gpx1^tm1Ysh/Gpx1^tm1Ysh Gpx2^tm2Coh/Gpx2^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2652737
cx9	Gpx1^tm1Ysh/Gpx1^tm1Ysh Gpx2^tm2Coh/Gpx2^tm2Coh	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:2652736
cx10	Gpx1^tm1Ysh/Gpx1^tm1Ysh Sord^C57BL/Lia/Sord^C57BL/Lia	involves: 129S1/Sv * 129X1/SvJ * C57BL/LiA	MGI:3836917

Genotype
MGI:2652385

hm1

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh
• Genetic Background: B6.129-Gpx1^tm1Ysh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

abnormal jejunum crypts of Lieberkuhn morphology ( J:63988 )

• Background Sensitivity: the jejunum crypt, but not ileal crypts, are more resistant to gamma radiation induced damage than controls

cellular

decreased cellular sensitivity to gamma-irradiation ( J:63988 )

• Background Sensitivity: the jejunum crypt, but not ileal crypts, are more resistant to gamma radiation induced damage than controls

endocrine/exocrine glands

abnormal jejunum crypts of Lieberkuhn morphology ( J:63988 )

• Background Sensitivity: the jejunum crypt, but not ileal crypts, are more resistant to gamma radiation induced damage than controls

Genotype
MGI:3653634

hm2

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

homeostasis/metabolism

abnormal catalase activity ( J:110762 )

• catalase activity varies considerably among tissues examined, from only 6% of the Gpx1 activity in the lens to 222% in the liver

• the lens has the lowest Gpx1 and catalase activities of any tissue based on activity per mg of total lens protein

decreased glutathione peroxidase activity ( J:110762 )

• glutathione peroxidase activity is very low or undetectable in mutants in the lens or other tissues

• the lens has the lowest Gpx1 and catalase activities of any tissue based on activity per mg of total lens protein

vision/eye

cataract ( J:142446 )

• 13% of mice develop cataracts between 3 and 4 months of age

Genotype
MGI:2176730

hm3

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

digestive/alimentary system

abnormal ileum crypts of Lieberkuhn morphology ( J:63988 )

• Background Sensitivity: ileal crypts are more resistant to gamma radiation induced damage than controls

abnormal jejunum crypts of Lieberkuhn morphology ( J:63988 )

• Background Sensitivity: jejunum crypts are more resistant to gamma radiation induced damage than controls

cellular

decreased cellular sensitivity to gamma-irradiation ( J:63988 )

• Background Sensitivity: ileal crypts and the jejunum crypt are more resistant to gamma radiation induced damage than controls

hematopoietic system

abnormal platelet physiology ( J:41169 )

• platelets incubated with arachidonic acid generate less 12-hydroxyeicosatetraenoic acid and more polar products relative to control platelets at a higher concentration of arachidonic acid, however homozygotes are healthy and fertile, show no increase in sensitivity to hyperoxia, have no abnormalities in the brain, heart, intestine, kidney, liver, lung, and spleen, and have normal numbers of total blood cells, red cells, reticulocytes, lymphocytes, monocytes, neutrophils, eosinophils, and platelets

homeostasis/metabolism

abnormal platelet physiology ( J:41169 )

• platelets incubated with arachidonic acid generate less 12-hydroxyeicosatetraenoic acid and more polar products relative to control platelets at a higher concentration of arachidonic acid, however homozygotes are healthy and fertile, show no increase in sensitivity to hyperoxia, have no abnormalities in the brain, heart, intestine, kidney, liver, lung, and spleen, and have normal numbers of total blood cells, red cells, reticulocytes, lymphocytes, monocytes, neutrophils, eosinophils, and platelets

endocrine/exocrine glands

abnormal ileum crypts of Lieberkuhn morphology ( J:63988 )

• Background Sensitivity: ileal crypts are more resistant to gamma radiation induced damage than controls

abnormal jejunum crypts of Lieberkuhn morphology ( J:63988 )

• Background Sensitivity: jejunum crypts are more resistant to gamma radiation induced damage than controls

hearing/vestibular/ear

cochlear inner hair cell degeneration ( J:113167 )

• at 4 weeks after noise exposure, homozygotes display a more severe IHC loss in the most basal 40% of the cochlea than wild-type mice

cochlear outer hair cell degeneration ( J:113167 )

• at 4 weeks after noise exposure, homozygotes display a more severe OHC loss in the most basal 40% of the cochlea than wild-type mice

increased or absent threshold for auditory brainstem response ( J:113167 )

• prior to noise exposure, 2-month-old homozygotes exhibit significantly higher ABR thresholds only at 28.3 kHz (16.4 dB), but not at 5 kHz (1.3 dB), 10 kHz (0.3 dB) or 20 kHz (8.3 dB), relative to wild-type mice

increased susceptibility to noise-induced hearing loss ( J:113167 )

• at 4 weeks after broadband noise exposure (110 dB SPL; 1 hr), 2-month-old homozygotes display up to 15 dB higher ABR thresholds than wild-type mice across all test frequencies, with significant ABR elevations at 20 kHz (~23 db) and 28.3 kHz (~15 dB)

• the contribution of null genotype to noise-induced hearing loss ranges from ~0 dB at 5 kHz to an estimated 15 dB at 20 kHz

nervous system

cochlear inner hair cell degeneration ( J:113167 )

• at 4 weeks after noise exposure, homozygotes display a more severe IHC loss in the most basal 40% of the cochlea than wild-type mice

cochlear outer hair cell degeneration ( J:113167 )

• at 4 weeks after noise exposure, homozygotes display a more severe OHC loss in the most basal 40% of the cochlea than wild-type mice

cochlear ganglion degeneration ( J:113167 )

• at 4 weeks after noise exposure, homozygotes exhibit a ~50% greater loss of nerve fibers per habenula in the cochlear base than wild-type mice

Genotype
MGI:3653633

ht4

• Allelic Composition: Gpx1^tm1Ysh/Gpx1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:110762 )

• heterozygous mice show a 40-50% reduction is Gpx1 activity in various tissues compared to wild-type

Genotype
MGI:5527284

cx5

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh; Prkn^tm1Shn/Prkn^tm1Shn; Park7^tm1Shn/Park7^tm1Shn
• Genetic Background: B6.Cg-Park7^tm1Shn Gpx1^tm1Ysh Prkn^tm1Shn

behavior/neurological

impaired coordination ( J:202221 )

• increased latency to fall (rotarod test) observed at 12 and 18 months of age as compared to controls

homeostasis/metabolism

increased dopamine level ( J:202221 )

• striatal dopamine levels are significantly elevated at 6, 12 and 18 months of age, however, dopamine turnover is similar to controls

increased serotonin level ( J:202221 )

• striatal serotonin levels are increased at 12 months of age, however, serotonin turnover is similar to controls

Genotype
MGI:4456209

cx6

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh; Gpx3^tm1Hjco/Gpx3^tm1Hjco
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:159745 )

N • mice tolerate a selenium-deficient diet

decreased glutathione peroxidase activity ( J:159745 )

• glutathione peroxidase activity is less than in Gpx3^tm1Jcwh homozygotes and is not sensitive to selenium status

Genotype
MGI:2652741

cx7

• Allelic Composition: Gpx1^tm1Ysh/Gpx1⁺; Gpx2^tm2Coh/Gpx2^tm2Coh
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

premature death ( J:71506 )

• 10% mortality before 35 days of age, however do not exhibit cachexia

digestive/alimentary system

perianal ulcer ( J:71506 )

• seen in 17% of mice

diarrhea ( J:71506 )

• seen in 17% of mice

intestinal inflammation ( J:71506 )

• seen in 50% of mice

immune system

intestinal inflammation ( J:71506 )

• seen in 50% of mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
inflammatory bowel disease		DOID:0050589	OMIM:PS266600	J:71506

Genotype
MGI:2652737

cx8

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh; Gpx2^tm2Coh/Gpx2⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

digestive/alimentary system

diarrhea ( J:71506 )

• seen in 10% of mice, however do not exhibit premature death

intestinal inflammation ( J:71506 )

• seen in 16% of mice

immune system

intestinal inflammation ( J:71506 )

• seen in 16% of mice

Genotype
MGI:2652736

cx9

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh; Gpx2^tm2Coh/Gpx2^tm2Coh
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

premature death ( J:71506 )

• 40% die or were euthanized due to poor health between 20-36 days of age

growth/size/body

decreased body weight ( J:71506 )

• gain weight more slowly or stop gaining weight completely starting around 16 days of age, however food intake is normal

cachexia ( J:71506 )

postnatal growth retardation ( J:71506 )

• onset of growth retardation is between 16 and 26 days of age

digestive/alimentary system

perianal ulcer ( J:71506 )

crypts of Lieberkuhn abscesses ( J:71506 )

• crypt abscesses are prevalent in the ileum, colon, and rectum

intestinal edema ( J:71506 )

• edematous colons

abnormal digestive system physiology ( J:71506 )

• anal mucus discharge

diarrhea ( J:71506 )

intestinal inflammation ( J:71506 )

• intestinal inflammation progresses from distal to proximal bowel in most homozygotes

colitis ( J:71506 )

• inflammation is limited to the mucosa

• 11 of 12 have distal colon colitis as early as 11 days of age

• 3 of 12 showed proximal colon colitis at 11-14 days of age

• most 15 day or older homozygotes have both distal and proximal colon inflammation

small intestinal inflammation ( J:71506 )

• severe inflammation of the ileum, but not jejunum or stomach , with inflammation limited to the mucosa

homeostasis/metabolism

intestinal edema ( J:71506 )

• edematous colons

decreased body temperature ( J:71506 )

• hypothermic under normal housing conditions and become even more hypothermic after being housed in metabolic cages (rest on grids without bedding for 24 hrs)

abnormal enzyme/coenzyme level ( J:71506 )

• higher levels of lipid hydroperoxide in the ileum and colon, but not jejunum

abnormal enzyme/coenzyme activity ( J:71506 )

• myeloperoxidase activity is increased in colonic mucosa

endocrine/exocrine glands

crypts of Lieberkuhn abscesses ( J:71506 )

• crypt abscesses are prevalent in the ileum, colon, and rectum

immune system

intestinal inflammation ( J:71506 )

• intestinal inflammation progresses from distal to proximal bowel in most homozygotes

colitis ( J:71506 )

• inflammation is limited to the mucosa

• 11 of 12 have distal colon colitis as early as 11 days of age

• 3 of 12 showed proximal colon colitis at 11-14 days of age

• most 15 day or older homozygotes have both distal and proximal colon inflammation

small intestinal inflammation ( J:71506 )

• severe inflammation of the ileum, but not jejunum or stomach , with inflammation limited to the mucosa

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
inflammatory bowel disease		DOID:0050589	OMIM:PS266600	J:71506

Genotype
MGI:3836917

cx10

• Allelic Composition: Gpx1^tm1Ysh/Gpx1^tm1Ysh; Sord^C57BL/Lia/Sord^C57BL/Lia
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/LiA

vision/eye

cataract ( J:142446 )

• cataract development is accelerated in these mice compared to mice homozygote null for just one locus

• 13% of mice have cataracts at 2 months of age, 16% of mice have cataracts at 3 months of age, and 26% of mice have cataracts at 4 months of age