Analysis Tools
immune system
| N |
• macrophage killing of Calmette-Guerin mycobacteria following ATP stimulation is normal
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Allele Symbol Allele Name Allele ID |
Nos2tm1Liew targeted mutation 1, Foo Y Liew MGI:2158787 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• macrophage killing of Calmette-Guerin mycobacteria following ATP stimulation is normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in vitro, splenocytes from L. major-infected homozygotes display significantly higher levels of T cell proliferation in response to leishmanial antigens or ConA than those from similarly-infected wild-type or heterozygous mice
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• homozygotes infected with L. major exhibit a significantly stronger Th1 type of immune response than similarly-infected wild-type or heterozygous mice
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• in vitro, splenocytes from L. major-infected homozygotes produce more IFN-gamma but less IL-4 in response to soluble leishmania antigens than those from similarly-infected wild-type or heterozygous mice
• in addition, splenocytes from carrageenin-treated homozygotes produce more IFN-gamma than those from similarly-treated wild-type mice when stimulated with ConA
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• in the carrageenin-induced paw-edema model, homozygotes develop significantly less footpad swelling than similarly-treated wild-type mice
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• unlike wild-type mice which display up to 12% loss of mass and ~50% mortality by 48 hr after LPS treatment (12.5 mg/kg), all homozygotes are shown to fully recover from an initial transient loss of mass within the first 24 hrs
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• unlike wild-type and heterozygous mice which are highly resistant to Leishmania major infection and achieve spontaneous healing after footpad injection, homozygotes display higher parasite loads and develop visceral disease by day 70 post-infection, as indicated by a 2- to 3-fold increase in spleen and draining lymph node size
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• in vitro, splenocytes from L. major-infected homozygotes display significantly higher levels of T cell proliferation in response to leishmanial antigens or ConA than those from similarly-infected wild-type or heterozygous mice
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• homozygotes infected with L. major exhibit a significantly stronger Th1 type of immune response than similarly-infected wild-type or heterozygous mice
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• in vitro, splenocytes from L. major-infected homozygotes display significantly higher levels of T cell proliferation in response to leishmanial antigens or ConA than those from similarly-infected wild-type or heterozygous mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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