Phenotypes associated with this allele

• Allele Symbol: Flt3l^tm1Imx
• Allele Name: targeted mutation 1, Immunex Corporation
• Allele ID: MGI:2158499
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Flt3l^tm1Imx/Flt3l^tm1Imx	C57BL/6-Flt3l^tm1Imx	MGI:3656072
hm2	Flt3l^tm1Imx/Flt3l^tm1Imx	involves: C57BL/6	MGI:5444882

Genotype
MGI:3656072

hm1

• Allelic Composition: Flt3l^tm1Imx/Flt3l^tm1Imx
• Genetic Background: C57BL/6-Flt3l^tm1Imx

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased IgM level ( J:62536 )

• other immune globulins and cell surface IgM are all normal

abnormal immune system cell morphology ( J:62536 )

impaired myelopoiesis ( J:62536 )

• reduced numbers of immature myeloid cells in bone marrow

abnormal leukocyte morphology ( J:62536 )

abnormal immature B cell morphology ( J:62536 )

• in bone marrow

decreased pro-B cell number ( J:62536 , J:79569 )

• 75-90% reduction in B cell precursor cells, both frequency and absolute numbers (J:62536)

• preferentially reduced in number (J:79569)

increased neutrophil cell number ( J:62536 )

• neutrophiles increased by about 20%

decreased leukocyte cell number ( J:62536 )

• WBC reduced 45% in peripheral blood

decreased plasmacytoid dendritic cell number ( J:62536 , J:89868 )

• lymphoid related dendritic cells are reduced in the spleen, thymus, and lymph nodes (J:62536)

• reduced in numbers in parenchymal organs such as the heart but not in skin (J:89868)

• less extensive dendrites on all dendritic cells (J:89868)

decreased lymphocyte cell number ( J:62536 )

• proportion of lymphocytes reduced significantly by about 76%

decreased double-negative T cell number ( J:79569 )

• pro-T cells are reduced up to 72% in the thymus whereas more mature pre-T cells are present in more normal numbers

decreased B cell number ( J:79569 )

• slight but significant reduction in peripheral B cell numbers

decreased pre-B cell number ( J:79569 )

• preferentially reduced in number

decreased NK cell number ( J:62536 )

• numbers of natural killer cells in the spleen are dramatically reduced

abnormal dendritic cell morphology ( J:62536 , J:89868 )

decreased myeloid dendritic cell number ( J:62536 , J:89868 )

• myeloid related dendritic cells reduced in spleen, thymus, and lymph nodes (J:62536)

• reduced in numbers in parenchymal organs such as the heart but not in skin (J:89868)

• less extensive dendrites on all dendritic cells (J:89868)

abnormal immune system organ morphology ( J:62536 )

spleen hypoplasia ( J:62536 )

• reduced by 30%

• both T and B cell numbers are reduced but the ratio of T to B cells is unchanged

• organ architecture remains normal

lymph node hypoplasia ( J:62536 )

• reduced by 35%

• both T and B cell numbers are reduced but the ratio of T to B cells is unchanged

• organ architecture remains normal

increased length of allograft survival ( J:89868 )

• allographs of hearts to BALB/c recipients show prolonged survival but not skin grafts

hematopoietic system

abnormal common myeloid progenitor cell morphology ( J:62536 )

• reduced absolute number of myeloid lineage precursors in bone marrow

impaired myelopoiesis ( J:62536 )

• reduced numbers of immature myeloid cells in bone marrow

decreased bone marrow cell number ( J:62536 )

• cellularity reduced by 27%

abnormal common lymphocyte progenitor cell morphology ( J:79569 )

• earliest common lymphoid cell precursors in bone marrow are reduced 10 fold

decreased hematopoietic stem cell number ( J:62536 )

• multipotent precursors reduced by 39% in bone marrow

abnormal leukocyte morphology ( J:62536 )

abnormal immature B cell morphology ( J:62536 )

• in bone marrow

decreased pro-B cell number ( J:62536 , J:79569 )

• 75-90% reduction in B cell precursor cells, both frequency and absolute numbers (J:62536)

• preferentially reduced in number (J:79569)

increased neutrophil cell number ( J:62536 )

• neutrophiles increased by about 20%

decreased leukocyte cell number ( J:62536 )

• WBC reduced 45% in peripheral blood

decreased plasmacytoid dendritic cell number ( J:62536 , J:89868 )

• lymphoid related dendritic cells are reduced in the spleen, thymus, and lymph nodes (J:62536)

• reduced in numbers in parenchymal organs such as the heart but not in skin (J:89868)

• less extensive dendrites on all dendritic cells (J:89868)

decreased lymphocyte cell number ( J:62536 )

• proportion of lymphocytes reduced significantly by about 76%

decreased double-negative T cell number ( J:79569 )

• pro-T cells are reduced up to 72% in the thymus whereas more mature pre-T cells are present in more normal numbers

decreased B cell number ( J:79569 )

• slight but significant reduction in peripheral B cell numbers

decreased pre-B cell number ( J:79569 )

• preferentially reduced in number

decreased NK cell number ( J:62536 )

• numbers of natural killer cells in the spleen are dramatically reduced

abnormal dendritic cell morphology ( J:62536 , J:89868 )

decreased myeloid dendritic cell number ( J:62536 , J:89868 )

• myeloid related dendritic cells reduced in spleen, thymus, and lymph nodes (J:62536)

• reduced in numbers in parenchymal organs such as the heart but not in skin (J:89868)

• less extensive dendrites on all dendritic cells (J:89868)

spleen hypoplasia ( J:62536 )

• reduced by 30%

• both T and B cell numbers are reduced but the ratio of T to B cells is unchanged

• organ architecture remains normal

increased IgM level ( J:62536 )

• other immune globulins and cell surface IgM are all normal

Genotype
MGI:5444882

hm2

• Allelic Composition: Flt3l^tm1Imx/Flt3l^tm1Imx
• Genetic Background: involves: C57BL/6

cellular

abnormal dendritic cell chemotaxis ( J:223508 )

• CD11b^hi dendritic cells do not migrate to mediastinal lymph nodes following LPS instillation

immune system

abnormal dendritic cell chemotaxis ( J:223508 )

• CD11b^hi dendritic cells do not migrate to mediastinal lymph nodes following LPS instillation

increased neutrophil cell number ( J:189122 )

• diphtheria toxin-treated mice exhibit increased numbers of splenic Ly6G+ neutrophils compared with controls

decreased dendritic cell number ( J:189122 )

• diphtheria toxin-treated mice exhibit complete ablation of all CD11c^hiMHCII+ dendritic cells regardless of pre-dendritic cell or monocyte origin

• reduced Lin-CD11c^hi classical dendritic cells in diphtheria-treated T. gondii-infected bone marrow chimeras

decreased CD103-positive CD11b-low dendritic cell number ( J:223508 )

• mice lack CD103+ dendritic cells in the lungs in steady state conditions and following OVA/LPS instillation

decreased CD11b-high dendritic cell number ( J:223508 )

• lungs contain a slightly reduced number of CD11b^hi dendritic cells as compared to wild-type

• CD11b^hi dendritic cells are comprised mostly of the CD14^hi subset with few CD14^med/lo cells

• mediastinal lymph nodes lack CD11b^hi dendritic cells following LPS instillation

decreased plasmacytoid dendritic cell number ( J:189122 )

• in diphtheria toxin-treated mice

decreased NK cell number ( J:189122 )

• in diphtheria toxin-treated mice

decreased macrophage cell number ( J:189122 )

• in the splenic red pulp of diphtheria toxin treated bone marrow chimeras

• F4/80+ red pulp macrophage are absent in diphtheria toxin-treated mice

• CD169+ marginal zone macrophages are almost absent in the spleen of diphtheria toxin-treated mice

• lymph node subscapular sinus macrophages are reduced in diphtheria toxin-treated mice

• however, F4/80+ medullar macrophages in the skin lymph nodes are unaffected in diphtheria toxin-treated mice

decreased monocyte cell number ( J:189122 )

• in diphtheria toxin-treated mice

abnormal T-helper 1 physiology ( J:189122 )

• reduced IFNgamma+ CD3+CD4+ T cells in diphtheria toxin-treated bone marrow chimeras immunized with either alpha-DEC-205-GAGp24 or alpha-Treml4-GAGp24, or infected with T. gondii

abnormal memory T cell physiology ( J:189122 )

• diphtheria toxin-treated bone marrow chimeras exhibit fail to exhibit an antitumor memory response unlike control mice and Zbtb46^{tm1(HBEGF)Mnz} heterozygotes

abnormal dendritic cell antigen presentation ( J:223508 )

• TH2, but not TH1, induction by dendritic cells is impaired

increased susceptibility to parasitic infection ( J:189122 )

• diphtheria toxin-treated mice infected with T. gondii exhibit decreased IFNgamma+ CD3+CD4+ T cells and increased parasitic burden compared with controls and Zbtb46^{tm1(HBEGF)Mnz} heterozygotes

hematopoietic system

abnormal dendritic cell chemotaxis ( J:223508 )

• CD11b^hi dendritic cells do not migrate to mediastinal lymph nodes following LPS instillation

increased neutrophil cell number ( J:189122 )

• diphtheria toxin-treated mice exhibit increased numbers of splenic Ly6G+ neutrophils compared with controls

decreased dendritic cell number ( J:189122 )

• diphtheria toxin-treated mice exhibit complete ablation of all CD11c^hiMHCII+ dendritic cells regardless of pre-dendritic cell or monocyte origin

• reduced Lin-CD11c^hi classical dendritic cells in diphtheria-treated T. gondii-infected bone marrow chimeras

decreased CD103-positive CD11b-low dendritic cell number ( J:223508 )

• mice lack CD103+ dendritic cells in the lungs in steady state conditions and following OVA/LPS instillation

decreased CD11b-high dendritic cell number ( J:223508 )

• lungs contain a slightly reduced number of CD11b^hi dendritic cells as compared to wild-type

• CD11b^hi dendritic cells are comprised mostly of the CD14^hi subset with few CD14^med/lo cells

• mediastinal lymph nodes lack CD11b^hi dendritic cells following LPS instillation

decreased plasmacytoid dendritic cell number ( J:189122 )

• in diphtheria toxin-treated mice

decreased NK cell number ( J:189122 )

• in diphtheria toxin-treated mice

decreased macrophage cell number ( J:189122 )

• in the splenic red pulp of diphtheria toxin treated bone marrow chimeras

• F4/80+ red pulp macrophage are absent in diphtheria toxin-treated mice

• CD169+ marginal zone macrophages are almost absent in the spleen of diphtheria toxin-treated mice

• lymph node subscapular sinus macrophages are reduced in diphtheria toxin-treated mice

• however, F4/80+ medullar macrophages in the skin lymph nodes are unaffected in diphtheria toxin-treated mice

decreased monocyte cell number ( J:189122 )

• in diphtheria toxin-treated mice

abnormal T-helper 1 physiology ( J:189122 )

• reduced IFNgamma+ CD3+CD4+ T cells in diphtheria toxin-treated bone marrow chimeras immunized with either alpha-DEC-205-GAGp24 or alpha-Treml4-GAGp24, or infected with T. gondii

abnormal memory T cell physiology ( J:189122 )

• diphtheria toxin-treated bone marrow chimeras exhibit fail to exhibit an antitumor memory response unlike control mice and Zbtb46^{tm1(HBEGF)Mnz} heterozygotes