mortality/aging
• homozygous mutant embryos die prior to E7.5
• at E7.5, 5 of 16 mutant embryos are found to be in an advanced stage of resorption
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embryo
• in vitro, homozygous mutant blastocysts display reduced ICM growth after 2 days in culture
• after 4 days in culture, 3 of 12 mutant outgrowths are significantly smaller than those of wild-type controls
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• at E6.5, homozygous mutant embryos are noticeably smaller than wild-type embryos
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• at E6.5, 3 of 10 homozygous mutant embryos lack an embryonic ectoderm
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• at E6.5, 3 of 10 homozygous mutant embryos display disorganized and degenerating cells
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• 9 of 12 homozygous mutant embryos lack any detectable ICM after 4 days in culture
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• at E6.5, 3 of 10 homozygous mutant embryos lack an extraembryonic ectoderm
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• at E6.5, 3 of 10 homozygous mutant embryos lack a preamniotic cavity
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growth/size/body
• at E6.5, homozygous mutant embryos are noticeably smaller than wild-type embryos
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cellular
• in vitro, homozygous mutant blastocysts display reduced ICM growth after 2 days in culture
• after 4 days in culture, 3 of 12 mutant outgrowths are significantly smaller than those of wild-type controls
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