All Phenotypes H2ax<tm1Nus> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	H2ax^tm1Nus/H2ax^tm1Nus	Not Specified	MGI:3040396
cn2	H2ax^tm1Nus/H2ax^tm2.1Fwa Tg(Cdh5-cre)7Mlia/0	involves: 129S6/SvEvTac * FVB/N	MGI:3849332
cx3	H2ax^tm1Nus/H2ax^tm1Nus Igh^tm1Mnz/Igh^tm1Mnz	involves: 129P2/OlaHsd	MGI:3712649
cx4	H2ax^tm1Nus/H2ax^tm1Nus Igh^tm2Mnz/Igh^tm2Mnz	involves: 129P2/OlaHsd	MGI:3712650
cx5	H2ax^tm1Nus/H2ax^tm1Nus Trp53^tm1Brd/Trp53^tm1Brd	involves: 129S7/SvEvBrd * C57BL/6J	MGI:3040401
cx6	H2ax^tm1Nus/H2ax⁺ Trp53^tm1Brd/Trp53^tm1Brd	involves: 129S7/SvEvBrd * C57BL/6J	MGI:3040402

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased mortality induced by gamma-irradiation ( J:76360 )

• 100% of mutants die within 11 days of exposure to gamma-irradiation compared to 20% of wild-type littermates

• irradiation induced more chromosomal breaks in mutants than in wild-type littermates

cellular

increased vascular endothelial cell apoptosis ( J:149487 )

• in a model of hypoxia driven tumor neovascularization, endothelial cell apoptosis is increased

decreased cell proliferation ( J:76360 , J:149487 )

• mouse embryo fibroblasts proliferate poorly in vitro with a marked increase in chromatid breaks (J:76360)

• primary lung epithelial cells display reduced growth factor stimulated proliferation under hypoxic conditions compared to wild-type control cells (J:149487)

decreased vascular endothelial cell proliferation ( J:194603 )

• in a model of hypoxia driven tumor neovascularization, endothelial cell proliferation is decreased

growth/size/body

postnatal growth retardation ( J:76360 )

• mutants are smaller than wild-type mice

immune system

abnormal class switch recombination ( J:120658 , J:194603 )

• switching is impaired (J:120658)

• however, class switching recombination (CSR)-induced Smu internal deletion, switching region accessibility and switching recombination junctions are normal (J:120658)

• impaired but partially rescued by short hairpin RNA inhibition of Rbbp8 and enhanced by treatment with the WRNi helicase inhibitor or to a lesser extent the ATM inhibitor Ku55933 (J:194603)

decreased B cell number ( J:76360 )

• a 50% reduction in the number of B cells is seen without any specific block in development

• an immunoglobin heavy chain class-switch recombination defect is the major cause of the decrease in expression of secondary isotypes of B cells in mutants

decreased T cell number ( J:76360 )

• a 50% reduction in the number of T cells is seen without any specific block in development

decreased IgG level ( J:73342 )

• impaired region specific DNA recombination involved in switching immunoglobin heavy chain constant regions results in a 50 - 86% reduction in surface IgG1

reproductive system

small seminiferous tubules ( J:76360 )

• the diameter of the seminiferous tubules is reduced in mutants compared to wild-type mice

small testis ( J:76360 )

• at 2 months mutant testes are half the size of wild-type testes

arrest of male meiosis ( J:76360 )

• spermatocytes are arrested at the pachytene stage of meiosis I in mutants

cardiovascular system

retina neovascularization ( J:149487 )

• neovascularization is impaired compared to wild-type controls following induced hind limb ischemia

abnormal physiological neovascularization ( J:149487 )

• neovascularization is impaired compared to wild-type controls following induced hind limb ischemia

abnormal tumor vascularization ( J:149487 )

• vascularization of implanted tumors is reduced compared to controls

increased vascular endothelial cell apoptosis ( J:149487 )

• in a model of hypoxia driven tumor neovascularization, endothelial cell apoptosis is increased

decreased vascular endothelial cell proliferation ( J:194603 )

• in a model of hypoxia driven tumor neovascularization, endothelial cell proliferation is decreased

neoplasm

abnormal tumor vascularization ( J:149487 )

• vascularization of implanted tumors is reduced compared to controls

decreased tumor growth/size ( J:149487 )

• both the growth and final weight of implanted Lewis lung carcinomas are reduced compared to controls

hematopoietic system

abnormal class switch recombination ( J:120658 , J:194603 )

• switching is impaired (J:120658)

• however, class switching recombination (CSR)-induced Smu internal deletion, switching region accessibility and switching recombination junctions are normal (J:120658)

• impaired but partially rescued by short hairpin RNA inhibition of Rbbp8 and enhanced by treatment with the WRNi helicase inhibitor or to a lesser extent the ATM inhibitor Ku55933 (J:194603)

decreased B cell number ( J:76360 )

• a 50% reduction in the number of B cells is seen without any specific block in development

• an immunoglobin heavy chain class-switch recombination defect is the major cause of the decrease in expression of secondary isotypes of B cells in mutants

decreased T cell number ( J:76360 )

• a 50% reduction in the number of T cells is seen without any specific block in development

decreased IgG level ( J:73342 )

• impaired region specific DNA recombination involved in switching immunoglobin heavy chain constant regions results in a 50 - 86% reduction in surface IgG1

endocrine/exocrine glands

small seminiferous tubules ( J:76360 )

• the diameter of the seminiferous tubules is reduced in mutants compared to wild-type mice

small testis ( J:76360 )

• at 2 months mutant testes are half the size of wild-type testes

vision/eye

retina neovascularization ( J:149487 )

• neovascularization is impaired compared to wild-type controls following induced hind limb ischemia

homeostasis/metabolism

increased mortality induced by gamma-irradiation ( J:76360 )

• 100% of mutants die within 11 days of exposure to gamma-irradiation compared to 20% of wild-type littermates

• irradiation induced more chromosomal breaks in mutants than in wild-type littermates

Allelic Composition	H2ax^tm1Nus/H2ax^tm2.1Fwa Tg(Cdh5-cre)7Mlia/0
Genetic Background	involves: 129S6/SvEvTac * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2ax^tm1Nus mutation (1 available); any H2ax mutation (7 available)
H2ax^tm2.1Fwa mutation (0 available); any H2ax mutation (7 available)
Tg(Cdh5-cre)7Mlia mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

retina neovascularization ( J:149487 )

• hypoxia induced retinal neovascularization is decreased compared to controls

abnormal tumor vascularization ( J:149487 )

• vascularization of implanted tumors is reduced compared to controls

neoplasm

abnormal tumor vascularization ( J:149487 )

• vascularization of implanted tumors is reduced compared to controls

decreased tumor growth/size ( J:149487 )

• growth of implanted Lewis lung carcinomas is reduced compared to controls

vision/eye

retina neovascularization ( J:149487 )

• hypoxia induced retinal neovascularization is decreased compared to controls

Allelic Composition	H2ax^tm1Nus/H2ax^tm1Nus Igh^tm1Mnz/Igh^tm1Mnz
Genetic Background	involves: 129P2/OlaHsd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2ax^tm1Nus mutation (1 available); any H2ax mutation (7 available)
Igh^tm1Mnz mutation (4 available); any Igh mutation (43 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:120658 )

N	• the rate of somatic hypermutation is normal

Allelic Composition	H2ax^tm1Nus/H2ax^tm1Nus Igh^tm2Mnz/Igh^tm2Mnz
Genetic Background	involves: 129P2/OlaHsd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2ax^tm1Nus mutation (1 available); any H2ax mutation (7 available)
Igh^tm2Mnz mutation (1 available); any Igh mutation (43 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:120658 )

N	• the rate of somatic hypermutation is normal

Allelic Composition	H2ax^tm1Nus/H2ax^tm1Nus Trp53^tm1Brd/Trp53^tm1Brd
Genetic Background	involves: 129S7/SvEvBrd * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2ax^tm1Nus mutation (1 available); any H2ax mutation (7 available)
Trp53^tm1Brd mutation (5 available); any Trp53 mutation (232 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:86590 )

• onset of death is 8 weeks for double homozygotes significantly earlier than for mice with only Trp53^tm1Brd

neoplasm

increased lymphoma incidence ( J:86590 )

• in double homozygotes T and B cell lymphomas predominate

Allelic Composition	H2ax^tm1Nus/H2ax⁺ Trp53^tm1Brd/Trp53^tm1Brd
Genetic Background	involves: 129S7/SvEvBrd * C57BL/6J

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:86590 )

• onset of death is 10 weeks for double homozygotes significantly earlier than for mice with only Trp53^tm1Brd

neoplasm

increased incidence of induced tumors ( J:86590 )

• these mice have a broader tumor spectrum than the double homozygotes that includes thymic lymphomas. sarcomas, leukemia, and brain tumors

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23